Restriction map for
pF1KE0294
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AccI 865 GTCTAC GT^[AC][GT]AC
AciI 780 GCGG CCGC(-3/-1)
AfaI 396 GTAC GT^AC
AspHI 67 GAGCTC G[AT]GC[AT]^C
AvaII 249 GGTCC G^G[AT]CC
BanII 67 GAGCTC G[GA]GC[CT]^C
BmyI 67 GAGCTC G[AGT]GC[ACT]^C
BseRI 340 CTCCTC GAGGAG(10/8)
BsgI 567 CTGCAC GTGCAG(16/14)
BsiHKAI 67 GAGCTC G[AT]GC[AT]^C
BsmI 917 GAATGC GAATGC(1/-1)
Bsp1286I 67 GAGCTC G[AGT]GC[ACT]^C
BspMI 254 ACCTGC ACCTGC(4/8)
BsrDI 345 CATTGC GCAATG(2/0)
BstAPI 329 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 683 AGATCC [GA]^GATC[CT]
EcoT14I 315 CCTTGG C^C[AT][AT]GG
MflI 683 AGATCC [GA]^GATC[CT]
NspI 497 ACATGC [GA]CATG^[CT]
RsaI 396 GTAC GT^AC
SacI 67 GAGCTC GAGCT^C
SfaNI 611 GCATC GCATC(5/9)
SfcI 867 CTACAG C^T[GA][CT]AG
StuI 707 AGGCCT AGG^CCT
StyI 315 CCTTGG C^C[AT][AT]GG
XcmI 275 CCATCTCCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 683 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 684 GATCC GGATC(4/5)
810 GGATC
AlwNI 73 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
241 CAGCTGCTG
BalI 122 TGGCCA TGG^CCA
476 TGGCCA
BanI 546 GGCACC G^G[CT][GA]CC
627 GGTGCC
BpmI 54 CTCCAG CTGGAG(16/14)
70 CTCCAG
BsaJI 60 CCTGGG C^C[ACGT][ACGT]GG
315 CCTTGG
HincII 523 GTTAAC GT[CT]^[GA]AC
667 GTTAAC
HindII 523 GTTAAC GT[CT]^[GA]AC
667 GTTAAC
HinfI 162 GACTC G^A[ACGT]TC
211 GAGTC
HpaI 523 GTTAAC GTT^AAC
667 GTTAAC
HphI 384 TCACC GGTGA(8/7)
716 TCACC
MboII 681 GAAGA GAAGA(8/7)
96 TCTTC
MluNI 122 TGGCCA TGG^CCA
476 TGGCCA
MscI 122 TGGCCA TGG^CCA
476 TGGCCA
PleI 162 GACTC GAGTC(4/5)
211 GAGTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI BbvCI BbvI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII ItaI MaeIII MboI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp45I Tsp509I TspRI