Restriction map for
pF1KE0294
(Circular) (Six-base) MAPSORT of: pF1KE0294.seq Check: 4617 from: 1 to: 4055
pF1KE0294 4055 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 371 371
Size: 4055
AccI GT'mk_AC
Cuts at: 974 1093 974
Size: 119 3936
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 808 1880 2312 3059 3327 246
Size: 562 1072 432 747 268 974
Fragments arranged by size:
1072 974 747 562 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AleI CACnn'nnGTG
Cuts at: 688 688
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 186 354 1360 2927 186
Size: 168 1006 1567 1314
Fragments arranged by size:
1567 1314 1006 168
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 1059 3408 3664 1059
Size: 2349 256 1450
Fragments arranged by size:
2349 1450 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 654 735 1071 1485 1733 1768 654
Size: 81 336 414 248 35 2941
Fragments arranged by size:
2941 414 336 248 81 35
BanII G_rGCy'C
Cuts at: 179 1069 2099 179
Size: 890 1030 2135
Fragments arranged by size:
2135 1030 890
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BbvCI CC'TCA_GC
Cuts at: 347 715 798 803 347
Size: 368 83 5 3599
Fragments arranged by size:
3599 368 83 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4021
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 1680 1773 2829 1680
Size: 93 1056 2906
Fragments arranged by size:
2906 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 163 147
Size: 16 4039
Bpu10I CC'TnA_GC
Cuts at: 347 715 798 803 347
Size: 368 83 5 3599
Fragments arranged by size:
3599 368 83 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 439
Size: 4055
BseYI C'CCAG_C
Cuts at: 407 528 1480 2238 2815 3311 407
Size: 121 952 758 577 496 1151
Fragments arranged by size:
1151 952 758 577 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 660
Size: 4055
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 1069 1847 2037 2829 179
Size: 890 778 190 792 1405
Fragments arranged by size:
1405 890 792 778 190
BsmI GAATG_Cn'
Cuts at: 1031 1031
Size: 4055
Bsp1286I G_dGCh'C
Cuts at: 179 1069 1680 1773 1847 2037 2099 2829 179
Size: 890 611 93 74 190 62 730 1405
Fragments arranged by size:
1405 890 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 1105 1319 1621 2002 371
Size: 734 214 302 381 2424
Fragments arranged by size:
2424 734 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 452 1967 452
Size: 1515 2540
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 443 1432 443
Size: 989 3066
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstYI r'GATC_y
Cuts at: 1 791 1080 1547 1570 1905 2151 3163 3249
Size: 790 289 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 790 467 449 346 335 289 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 938 1298 938
Size: 360 3695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 209 244 209
Size: 35 4020
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 230 248 584 1640 1814 2205 2232 3577 230
Size: 18 336 1056 174 391 27 1345 708
Fragments arranged by size:
1345 1056 708 391 336 174 27 18
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 177 1067 177
Size: 890 3165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 163 246 808 1880 2312 3059 3327 147
Size: 16 83 562 1072 432 747 268 875
Fragments arranged by size:
1072 875 747 562 432 268 83 16
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 834 2074 834
Size: 1240 2815
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1897 1927 1929 1959 1897
Size: 30 2 30 3993
Fragments arranged by size:
3993 30 30 2
HincII GTy'rAC
Cuts at: 633 777 1094 633
Size: 144 317 3594
Fragments arranged by size:
3594 317 144
HpaI GTT'AAC
Cuts at: 633 777 633
Size: 144 3911
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 515 2726 2910 515
Size: 2211 184 1660
Fragments arranged by size:
2211 1660 184
MscI TGG'CCA
Cuts at: 232 586 1816 232
Size: 354 1230 2471
Fragments arranged by size:
2471 1230 354
MslI CAynn'nnrTG
Cuts at: 151 283 457 688 2044 2171 151
Size: 132 174 231 1356 127 2035
Fragments arranged by size:
2035 1356 231 174 132 127
MspA1I CmG'CkG
Cuts at: 183 266 351 411 802 1166 1480 1840 2853
Size: 83 85 60 391 364 314 360 1013
Cuts at: 2853 3098 183
Size: 245 1140
Fragments arranged by size:
1140 1013 391 364 360 314 245 85
83 60
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 609 1108 1608 2515 609
Size: 499 500 907 2149
Fragments arranged by size:
2149 907 500 499
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 183 266 351 411 802 1480 1840 183
Size: 83 85 60 391 678 360 2398
Fragments arranged by size:
2398 678 391 360 85 83 60
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 179 1069 179
Size: 890 3165
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 975 1098 1783 2776 2967 33
Size: 942 123 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 191 123
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 1210 2125 2617 2879 1210
Size: 915 492 262 2386
Fragments arranged by size:
2386 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StuI AGG'CCT
Cuts at: 817 817
Size: 4055
StyI C'CwwG_G
Cuts at: 423 1189 1543 423
Size: 766 354 2935
Fragments arranged by size:
2935 766 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 700 1638 1771 2230 2439 421
Size: 279 938 133 459 209 2037
Fragments arranged by size:
2037 938 459 279 209 133
TatI w'GTAC_w
Cuts at: 3570 3570
Size: 4055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 131 163 1016 1048 3572 3604 131
Size: 32 853 32 2524 32 582
Fragments arranged by size:
2524 853 582 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 1550 390
Size: 1160 2895
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI