Restriction map for pF1KE0286
 (Linear) (Six-base) MAPSORT of: KIEE0286.seq  Check: 8027  from: 1  to: 207



KIEE0286.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AclI AA'CG_TT

Cuts at:      0      93     207 
   Size:         93     114
 
AflIII A'CryG_T

Cuts at:      0      58     207 
   Size:         58     149
 
ApoI r'AATT_y

Cuts at:      0     164     207 
   Size:        164      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     207 
   Size:        142      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     207 
   Size:        156      51
 
BsiEI CG_ry'CG

Cuts at:      0     102     207 
   Size:        102     105
 
BsmI GAATG_Cn'

Cuts at:      0      78     207 
   Size:         78     129
 
BstYI r'GATC_y

Cuts at:      0       5     207 
   Size:          5     202
 
NdeI CA'TA_TG

Cuts at:      0     145     207 
   Size:        145      62
 
NspI r_CATG'y

Cuts at:      0      62     207 
   Size:         62     145
 
PciI A'CATG_T

Cuts at:      0      58     207 
   Size:         58     149
 
SmlI C'TyrA_G

Cuts at:      0     121     207 
   Size:        121      86
 
StyI C'CwwG_G

Cuts at:      0      65     207 
   Size:         65     142
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      63     207 
   Size:         63     144
 

 Enzymes that do cut:

     AclI   AflIII     ApoI    BpuEI     BsgI    BsiEI     BsmI    BstYI
     NdeI     NspI     PciI     SmlI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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