Restriction map for pF1KE0278
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstYI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       FseI        FspI        HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MflI        MluI        
MroI        MseI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI          535  GTCTAC               GT^[AC][GT]AC
AluI          180  AGCT                 AG^CT
AocI          583  CCTGAGG              CC^T[ACGT]AGG
AvaII         378  GGACC                G^G[AT]CC
BclI          387  TGATCA               T^GATCA
BfaI          119  CTAG                 C^TAG
BpmI          111  CTCCAG               CTGGAG(16/14)
BspHI         491  TCATGA               T^CATGA
BspMI         595  ACCTGC               ACCTGC(4/8)
BsrI          311  ACTGG                ACTGG(1/-1)
BstEII        591  GGTCACC              G^GT[ACGT]ACC
BstPI         591  GGTCACC              G^GT[ACGT]ACC
Bsu36I        583  CCTGAGG              CC^T[ACGT]AGG
DpnII         388  GATC                 ^GATC
Eco81I        583  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       591  GGTCACC              G^GT[ACGT]ACC
EcoT22I       459  ATGCAT               ATGCA^T
FauI          622  CCCGC                CCCGC(4/6)
FbaI          387  TGATCA               T^GATCA
MaeI          119  CTAG                 C^TAG
MaeII         502  ACGT                 A^CGT
MboI          388  GATC                 ^GATC
NcoI          209  CCATGG               C^CATGG
NdeII         388  GATC                 ^GATC
NsiI          459  ATGCAT               ATGCA^T
RcaI          491  TCATGA               T^CATGA
Sau3AI        388  GATC                 ^GATC
TaqI          398  TCGA                 T^CGA
TthHB8I       398  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI          577  CCGC                 CCGC(-3/-1)
              623  CCGC                
AfaI          504  GTAC                 GT^AC
              516  GTAC                
ApaI           75  GGGCCC               GGGCC^C
              546  GGGCCC              
Asp700I       132  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              135  GAAGTTCTTC          
BalI          149  TGGCCA               TGG^CCA
              288  TGGCCA              
BanII          75  GGGCCC               G[GA]GC[CT]^C
              546  GGGCCC              
BmyI           75  GGGCCC               G[AGT]GC[ACT]^C
              546  GGGCCC              
Bsp1286I       75  GGGCCC               G[AGT]GC[ACT]^C
              546  GGGCCC              
CfoI          236  GCGC                 GCG^C
              616  GCGC                
DdeI          410  CTGAG                C^T[ACGT]AG
              584  CTGAG               
EaeI          149  TGGCCA               [CT]^GGCC[GA]
              288  TGGCCA              
HaeII         235  AGCGCC               [GA]GCGC^[CT]
              615  AGCGCC              
HhaI          236  GCGC                 GCG^C
              616  GCGC                
HinP1I        236  GCGC                 G^CGC
              616  GCGC                
MboII         132  GAAGA                GAAGA(8/7)
              140  TCTTC               
MluNI         149  TGGCCA               TGG^CCA
              288  TGGCCA              
MscI          149  TGGCCA               TGG^CCA
              288  TGGCCA              
MslI          367  CACCCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              458  CATGCATGTG          
NspI          457  ACATGC               [GA]CATG^[CT]
              461  GCATGT              
RsaI          504  GTAC                 GT^AC
              516  GTAC                
SfaNI          56  GATGC                GCATC(5/9)
              566  GCATC               
TspRI         408  CACTG                CA[GC]TG[ACGT][ACGT]^
              423  CAGTG               
XcmI          113  CCAGGTCTAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              296  CCAAGGATGAGGTGG     
XmnI          132  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              135  GAAGTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

BbvI        BsaJI       BseRI       BsiYI       BslI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DraII       DsaI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MaeIII      MnlI        MvaI        MwoI        NlaIII      
NlaIV       PstI        Sau96I      ScrFI       SfcI        
StyI        TseI        Tsp45I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com