(Circular) (Six-base) MAPSORT of: pF1KE0277.seq Check: 8887 from: 1 to: 4076 pF1KE0277 4076 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 1114 1114 Size: 4076 Acc65I G'GTAC_C Cuts at: 1092 1092 Size: 4076 AclI AA'CG_TT Cuts at: 3916 3916 Size: 4076 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 119 1901 2333 3080 3348 119 Size: 1782 432 747 268 847 Fragments arranged by size: 1782 847 747 432 268 AflII C'TTAA_G Cuts at: 799 799 Size: 4076 AflIII A'CryG_T Cuts at: 1428 2532 3509 1428 Size: 1104 977 1995 Fragments arranged by size: 1995 1104 977 AgeI A'CCGG_T Cuts at: 497 2464 497 Size: 1967 2109 AleI CACnn'nnGTG Cuts at: 869 913 869 Size: 44 4032 AlwNI CAG_nnn'CTG Cuts at: 1381 2948 1381 Size: 1567 2509 ApaLI G'TGCA_C Cuts at: 916 2846 916 Size: 1930 2146 ApoI r'AATT_y Cuts at: 159 625 1080 3429 3685 159 Size: 466 455 2349 256 550 Fragments arranged by size: 2349 550 466 455 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4076 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4076 AvaI C'yCGr_G Cuts at: 1096 1096 Size: 4076 BamHI G'GATC_C Cuts at: 383 1101 383 Size: 718 3358 BanI G'GyrC_C Cuts at: 1092 1506 1754 1789 1092 Size: 414 248 35 3379 Fragments arranged by size: 3379 414 248 35 BanII G_rGCy'C Cuts at: 572 1090 2120 572 Size: 518 1030 2528 Fragments arranged by size: 2528 1030 518 BbeI G_GCGC'C Cuts at: 1510 1758 1510 Size: 248 3828 BciVI GTATCCnnnnn_n' Cuts at: 1974 2735 4076 1974 Size: 761 1341 1974 Fragments arranged by size: 1974 1341 761 BclI T'GATC_A Cuts at: 1596 1596 Size: 4076 BglII A'GATC_T Cuts at: 1591 3628 1591 Size: 2037 2039 BlpI GC'TnA_GC Cuts at: 693 1188 693 Size: 495 3581 Bme1580I G_kGCm'C Cuts at: 920 1701 1794 2850 920 Size: 781 93 1056 2146 Fragments arranged by size: 2146 1056 781 93 BmrI ACTGGGnnnn_n' Cuts at: 426 1288 1369 1704 3848 426 Size: 862 81 335 2144 654 Fragments arranged by size: 2144 862 654 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2443 2443 Size: 4076 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1252 2131 2623 2921 1252 Size: 879 492 298 2407 Fragments arranged by size: 2407 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3780 3780 Size: 4076 BsaAI yAC'GTr Cuts at: 1429 1429 Size: 4076 BsaBI GATnn'nnATC Cuts at: 6 1590 3627 6 Size: 1584 2037 455 Fragments arranged by size: 2037 1584 455 BsaHI Gr'CG_yC Cuts at: 1507 1755 2457 3953 1507 Size: 248 702 1496 1630 Fragments arranged by size: 1630 1496 702 248 BsaWI w'CCGG_w Cuts at: 497 1265 1786 2464 2738 2885 3296 497 Size: 768 521 678 274 147 411 1277 Fragments arranged by size: 1277 768 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 219 469 219 Size: 250 3826 BseYI C'CCAG_C Cuts at: 378 1501 2259 2836 3332 378 Size: 1123 758 577 496 1122 Fragments arranged by size: 1123 1122 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 283 658 283 Size: 375 3701 BsiEI CG_ry'CG Cuts at: 104 1618 1664 2872 104 Size: 1514 46 1208 1308 Fragments arranged by size: 1514 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 920 1090 1868 2058 2850 920 Size: 170 778 190 792 2146 Fragments arranged by size: 2146 792 778 190 170 BsmI GAATG_Cn' Cuts at: 1046 1046 Size: 4076 Bsp1286I G_dGCh'C Cuts at: 572 920 1090 1701 1794 1868 2058 2120 2850 Size: 348 170 611 93 74 190 62 730 Cuts at: 2850 572 Size: 1798 Fragments arranged by size: 1798 730 611 348 190 170 93 74 62 BspEI T'CCGG_A Cuts at: 3296 3296 Size: 4076 BspHI T'CATG_A Cuts at: 3252 3252 Size: 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 862 1126 1340 1642 2023 862 Size: 264 214 302 381 2915 Fragments arranged by size: 2915 381 302 264 214 BsrBI CCG'CTC Cuts at: 50 2368 2422 3906 4071 50 Size: 2318 54 1484 165 55 Fragments arranged by size: 2318 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 425 503 1988 425 Size: 78 1485 2513 Fragments arranged by size: 2513 1485 78 BsrFI r'CCGG_y Cuts at: 497 2074 2255 2464 497 Size: 1577 181 209 2109 Fragments arranged by size: 2109 1577 209 181 BsrGI T'GTAC_A Cuts at: 368 368 Size: 4076 BssSI C'ACGA_G Cuts at: 2347 2705 2347 Size: 358 3718 BstAPI GCAn_nnn'nTGC Cuts at: 1453 1453 Size: 4076 BstBI TT'CG_AA Cuts at: 2437 2437 Size: 4076 BstYI r'GATC_y Cuts at: 1 383 681 1101 1568 1591 1926 2172 3184 Size: 382 298 420 467 23 335 246 1012 Cuts at: 3184 3270 3282 3628 1 Size: 86 12 346 449 Fragments arranged by size: 1012 467 449 420 382 346 335 298 246 86 23 12 BtgI C'CryG_G Cuts at: 242 447 944 242 Size: 205 497 3374 Fragments arranged by size: 3374 497 205 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2026 2207 2370 2026 Size: 181 163 3732 Fragments arranged by size: 3732 181 163 BtsI GCAGTG_nn' Cuts at: 1319 1319 Size: 4076 DraI TTT'AAA Cuts at: 1075 1075 Size: 4076 DrdI GACnn_nn'nnGTC Cuts at: 1782 2640 1782 Size: 858 3218 EaeI y'GGCC_r Cuts at: 1661 1835 2226 2253 3598 1661 Size: 174 391 27 1345 2139 Fragments arranged by size: 2139 1345 391 174 27 EagI C'GGCC_G Cuts at: 1661 1661 Size: 4076 EarI CTCTTCn'nnn_ Cuts at: 824 2099 2309 824 Size: 1275 210 2591 Fragments arranged by size: 2591 1275 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2284 2594 2740 3886 2284 Size: 310 146 1146 2474 Fragments arranged by size: 2474 1146 310 146 EcoICRI GAG'CTC Cuts at: 1088 1088 Size: 4076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 119 1901 2333 3080 3348 119 Size: 1782 432 747 268 847 Fragments arranged by size: 1782 847 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 676 676 Size: 4076 EcoO109I rG'GnC_Cy Cuts at: 1215 1215 Size: 4076 EcoRI G'AATT_C Cuts at: 159 1080 159 Size: 921 3155 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 4076 FspI TGC'GCA Cuts at: 1857 1857 Size: 4076 HaeII r_GCGC'y Cuts at: 1510 1758 2780 3759 1510 Size: 248 1022 979 1827 Fragments arranged by size: 1827 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1918 1948 1950 1980 1918 Size: 30 2 30 4014 Fragments arranged by size: 4014 30 30 2 HincII GTy'rAC Cuts at: 1115 1115 Size: 4076 KasI G'GCGC_C Cuts at: 1506 1754 1506 Size: 248 3828 KpnI G_GTAC'C Cuts at: 1096 1096 Size: 4076 MfeI C'AATT_G Cuts at: 258 258 Size: 4076 MluI A'CGCG_T Cuts at: 3509 3509 Size: 4076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 740 2747 2931 740 Size: 2007 184 1885 Fragments arranged by size: 2007 1885 184 MscI TGG'CCA Cuts at: 1837 1837 Size: 4076 MslI CAynn'nnrTG Cuts at: 869 913 2065 2192 869 Size: 44 1152 127 2753 Fragments arranged by size: 2753 1152 127 44 MspA1I CmG'CkG Cuts at: 1187 1501 1861 2874 3119 1187 Size: 314 360 1013 245 2144 Fragments arranged by size: 2144 1013 360 314 245 NaeI GCC'GGC Cuts at: 2257 2257 Size: 4076 NarI GG'CG_CC Cuts at: 1507 1755 1507 Size: 248 3828 NcoI C'CATG_G Cuts at: 242 447 242 Size: 205 3871 NgoMIV G'CCGG_C Cuts at: 2255 2255 Size: 4076 NspI r_CATG'y Cuts at: 1129 1629 2536 1129 Size: 500 907 2669 Fragments arranged by size: 2669 907 500 PciI A'CATG_T Cuts at: 2532 2532 Size: 4076 PmeI GTTT'AAAC Cuts at: 1075 1075 Size: 4076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3215 3247 3215 Size: 32 4044 PstI C_TGCA'G Cuts at: 1123 1808 1123 Size: 685 3391 PvuI CG_AT'CG Cuts at: 104 104 Size: 4076 PvuII CAG'CTG Cuts at: 1501 1861 1501 Size: 360 3716 RsrII CG'GwC_CG Cuts at: 2271 2271 Size: 4076 SacI G_AGCT'C Cuts at: 1090 1090 Size: 4076 SalI G'TCGA_C Cuts at: 1113 1113 Size: 4076 SapI GCTCTTCn'nnn_ Cuts at: 2099 2309 2099 Size: 210 3866 SbfI CC_TGCA'GG Cuts at: 1123 1123 Size: 4076 ScaI AGT'ACT Cuts at: 3593 3593 Size: 4076 SfcI C'TryA_G Cuts at: 33 1008 1119 1804 2797 2988 33 Size: 975 111 685 993 191 1121 Fragments arranged by size: 1121 993 975 685 191 111 SfoI GGC'GCC Cuts at: 1508 1756 1508 Size: 248 3828 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4076 SmaI CCC'GGG Cuts at: 1098 1098 Size: 4076 SmlI C'TyrA_G Cuts at: 799 1231 2146 2638 2900 799 Size: 432 915 492 262 1975 Fragments arranged by size: 1975 915 492 432 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4076 SphI G_CATG'C Cuts at: 1129 1629 1129 Size: 500 3576 StyI C'CwwG_G Cuts at: 242 447 1210 1564 242 Size: 205 763 354 2754 Fragments arranged by size: 2754 763 354 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1659 1792 2251 2460 1659 Size: 133 459 209 3275 Fragments arranged by size: 3275 459 209 133 TatI w'GTAC_w Cuts at: 368 3591 368 Size: 3223 853 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3593 3625 3593 Size: 32 4044 Tth111I GACn'n_nGTC Cuts at: 1873 1873 Size: 4076 XbaI T'CTAG_A Cuts at: 1107 1107 Size: 4076 XcmI CCAnnnn_n'nnnnTGG Cuts at: 249 958 1571 249 Size: 709 613 2754 Fragments arranged by size: 2754 709 613 XmaI C'CCGG_G Cuts at: 1096 1096 Size: 4076 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI