(Circular) (Six-base) MAPSORT of: pF1KE0274.seq Check: 8491 from: 1 to: 3440 pF1KE0274 3440 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AatII G_ACGT'C Cuts at: 208 208 Size: 3440 AccI GT'mk_AC Cuts at: 478 478 Size: 3440 Acc65I G'GTAC_C Cuts at: 456 456 Size: 3440 AclI AA'CG_TT Cuts at: 3280 3280 Size: 3440 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1265 1697 2444 2712 1265 Size: 432 747 268 1993 Fragments arranged by size: 1993 747 432 268 AfeI AGC'GCT Cuts at: 152 152 Size: 3440 AflII C'TTAA_G Cuts at: 298 298 Size: 3440 AflIII A'CryG_T Cuts at: 792 1896 2873 792 Size: 1104 977 1359 Fragments arranged by size: 1359 1104 977 AgeI A'CCGG_T Cuts at: 1828 1828 Size: 3440 AleI CACnn'nnGTG Cuts at: 121 121 Size: 3440 AlwNI CAG_nnn'CTG Cuts at: 745 2312 745 Size: 1567 1873 ApaLI G'TGCA_C Cuts at: 2210 2210 Size: 3440 ApoI r'AATT_y Cuts at: 193 412 444 2793 3049 193 Size: 219 32 2349 256 584 Fragments arranged by size: 2349 584 256 219 32 AseI AT'TA_AT Cuts at: 20 20 Size: 3440 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3440 AvaI C'yCGr_G Cuts at: 460 460 Size: 3440 BamHI G'GATC_C Cuts at: 465 465 Size: 3440 BanI G'GyrC_C Cuts at: 328 456 870 1118 1153 328 Size: 128 414 248 35 2615 Fragments arranged by size: 2615 414 248 128 35 BanII G_rGCy'C Cuts at: 454 1484 454 Size: 1030 2410 BbeI G_GCGC'C Cuts at: 874 1122 874 Size: 248 3192 BciVI GTATCCnnnnn_n' Cuts at: 1338 2099 3440 1338 Size: 761 1341 1338 Fragments arranged by size: 1341 1338 761 BclI T'GATC_A Cuts at: 960 960 Size: 3440 BglII A'GATC_T Cuts at: 955 2992 955 Size: 2037 1403 BlpI GC'TnA_GC Cuts at: 552 552 Size: 3440 Bme1580I G_kGCm'C Cuts at: 1065 1158 2214 1065 Size: 93 1056 2291 Fragments arranged by size: 2291 1056 93 BmgBI CAC'GTC Cuts at: 123 123 Size: 3440 BmrI ACTGGGnnnn_n' Cuts at: 652 733 1068 3212 652 Size: 81 335 2144 880 Fragments arranged by size: 2144 880 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1807 1807 Size: 3440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 139 295 139 Size: 156 3284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 616 1495 1987 2285 616 Size: 879 492 298 1771 Fragments arranged by size: 1771 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3144 3144 Size: 3440 BsaAI yAC'GTr Cuts at: 793 793 Size: 3440 BsaBI GATnn'nnATC Cuts at: 6 954 2991 6 Size: 948 2037 455 Fragments arranged by size: 2037 948 455 BsaHI Gr'CG_yC Cuts at: 205 871 1119 1821 3317 205 Size: 666 248 702 1496 328 Fragments arranged by size: 1496 702 666 328 248 BsaWI w'CCGG_w Cuts at: 251 629 1150 1828 2102 2249 2660 251 Size: 378 521 678 274 147 411 1031 Fragments arranged by size: 1031 678 521 411 378 274 147 BseYI C'CCAG_C Cuts at: 865 1623 2200 2696 865 Size: 758 577 496 1609 Fragments arranged by size: 1609 758 577 496 BsiEI CG_ry'CG Cuts at: 104 982 1028 2236 104 Size: 878 46 1208 1308 Fragments arranged by size: 1308 1208 878 46 BsiHKAI G_wGCw'C Cuts at: 128 454 1232 1422 2214 128 Size: 326 778 190 792 1354 Fragments arranged by size: 1354 792 778 326 190 BsmI GAATG_Cn' Cuts at: 166 429 166 Size: 263 3177 Bsp1286I G_dGCh'C Cuts at: 128 454 1065 1158 1232 1422 1484 2214 128 Size: 326 611 93 74 190 62 730 1354 Fragments arranged by size: 1354 730 611 326 190 93 74 62 BspEI T'CCGG_A Cuts at: 2660 2660 Size: 3440 BspHI T'CATG_A Cuts at: 2616 2616 Size: 3440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 490 704 1006 1387 490 Size: 214 302 381 2543 Fragments arranged by size: 2543 381 302 214 BsrBI CCG'CTC Cuts at: 50 275 1732 1786 3270 3435 50 Size: 225 1457 54 1484 165 55 Fragments arranged by size: 1484 1457 225 165 55 54 BsrDI GCAATG_nn' Cuts at: 140 1352 140 Size: 1212 2228 BsrFI r'CCGG_y Cuts at: 1438 1619 1828 1438 Size: 181 209 3050 Fragments arranged by size: 3050 209 181 BssSI C'ACGA_G Cuts at: 1711 2069 1711 Size: 358 3082 BstAPI GCAn_nnn'nTGC Cuts at: 817 817 Size: 3440 BstBI TT'CG_AA Cuts at: 1801 1801 Size: 3440 BstYI r'GATC_y Cuts at: 1 465 932 955 1290 1536 2548 2634 2646 Size: 464 467 23 335 246 1012 86 12 Cuts at: 2646 2992 1 Size: 346 449 Fragments arranged by size: 1012 467 464 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1390 1571 1734 1390 Size: 181 163 3096 Fragments arranged by size: 3096 181 163 BtsI GCAGTG_nn' Cuts at: 683 683 Size: 3440 DraI TTT'AAA Cuts at: 439 439 Size: 3440 DrdI GACnn_nn'nnGTC Cuts at: 1146 2004 1146 Size: 858 2582 EaeI y'GGCC_r Cuts at: 1025 1199 1590 1617 2962 1025 Size: 174 391 27 1345 1503 Fragments arranged by size: 1503 1345 391 174 27 EagI C'GGCC_G Cuts at: 1025 1025 Size: 3440 EarI CTCTTCn'nnn_ Cuts at: 287 1463 1673 287 Size: 1176 210 2054 Fragments arranged by size: 2054 1176 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 391 1648 1958 2104 3250 391 Size: 1257 310 146 1146 581 Fragments arranged by size: 1257 1146 581 310 146 EcoICRI GAG'CTC Cuts at: 452 452 Size: 3440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 139 295 1265 1697 2444 2712 139 Size: 156 970 432 747 268 867 Fragments arranged by size: 970 867 747 432 268 156 EcoO109I rG'GnC_Cy Cuts at: 579 579 Size: 3440 EcoRI G'AATT_C Cuts at: 412 444 412 Size: 32 3408 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 313 1459 313 Size: 1146 2294 FspI TGC'GCA Cuts at: 1221 1221 Size: 3440 HaeII r_GCGC'y Cuts at: 154 874 1122 2144 3123 154 Size: 720 248 1022 979 471 Fragments arranged by size: 1022 979 720 471 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 264 296 1282 1312 1314 1344 264 Size: 32 986 30 2 30 2360 Fragments arranged by size: 2360 986 32 30 30 2 HincII GTy'rAC Cuts at: 479 479 Size: 3440 KasI G'GCGC_C Cuts at: 870 1118 870 Size: 248 3192 KpnI G_GTAC'C Cuts at: 460 460 Size: 3440 MluI A'CGCG_T Cuts at: 2873 2873 Size: 3440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2111 2295 2111 Size: 184 3256 MscI TGG'CCA Cuts at: 1201 1201 Size: 3440 MslI CAynn'nnrTG Cuts at: 121 1429 1556 121 Size: 1308 127 2005 Fragments arranged by size: 2005 1308 127 MspA1I CmG'CkG Cuts at: 551 865 1225 2238 2483 551 Size: 314 360 1013 245 1508 Fragments arranged by size: 1508 1013 360 314 245 NaeI GCC'GGC Cuts at: 1621 1621 Size: 3440 NarI GG'CG_CC Cuts at: 871 1119 871 Size: 248 3192 NgoMIV G'CCGG_C Cuts at: 1619 1619 Size: 3440 NspI r_CATG'y Cuts at: 493 993 1900 493 Size: 500 907 2033 Fragments arranged by size: 2033 907 500 PciI A'CATG_T Cuts at: 1896 1896 Size: 3440 PflMI CCAn_nnn'nTGG Cuts at: 423 423 Size: 3440 PfoI T'CCnGG_A Cuts at: 155 416 155 Size: 261 3179 PmeI GTTT'AAAC Cuts at: 439 439 Size: 3440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2579 2611 2579 Size: 32 3408 PstI C_TGCA'G Cuts at: 487 1172 487 Size: 685 2755 PvuI CG_AT'CG Cuts at: 104 104 Size: 3440 PvuII CAG'CTG Cuts at: 865 1225 865 Size: 360 3080 RsrII CG'GwC_CG Cuts at: 1635 1635 Size: 3440 SacI G_AGCT'C Cuts at: 454 454 Size: 3440 SalI G'TCGA_C Cuts at: 477 477 Size: 3440 SapI GCTCTTCn'nnn_ Cuts at: 287 1463 1673 287 Size: 1176 210 2054 Fragments arranged by size: 2054 1176 210 SbfI CC_TGCA'GG Cuts at: 487 487 Size: 3440 ScaI AGT'ACT Cuts at: 2957 2957 Size: 3440 SfcI C'TryA_G Cuts at: 33 483 1168 2161 2352 33 Size: 450 685 993 191 1121 Fragments arranged by size: 1121 993 685 450 191 SfoI GGC'GCC Cuts at: 872 1120 872 Size: 248 3192 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3440 SmaI CCC'GGG Cuts at: 462 462 Size: 3440 SmlI C'TyrA_G Cuts at: 298 595 1510 2002 2264 298 Size: 297 915 492 262 1474 Fragments arranged by size: 1474 915 492 297 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3440 SphI G_CATG'C Cuts at: 493 993 493 Size: 500 2940 StuI AGG'CCT Cuts at: 212 212 Size: 3440 StyI C'CwwG_G Cuts at: 574 928 574 Size: 354 3086 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 358 1023 1156 1615 1824 358 Size: 665 133 459 209 1974 Fragments arranged by size: 1974 665 459 209 133 TatI w'GTAC_w Cuts at: 2955 2955 Size: 3440 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2957 2989 2957 Size: 32 3408 Tth111I GACn'n_nGTC Cuts at: 345 1237 345 Size: 892 2548 XbaI T'CTAG_A Cuts at: 471 471 Size: 3440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 363 935 363 Size: 572 2868 XmaI C'CCGG_G Cuts at: 460 460 Size: 3440 ZraI GAC'GTC Cuts at: 206 206 Size: 3440 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI