Restriction map for pF1KE0268
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstF5I      BstPI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DsaI        Eam1105I    Eco47III    EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FokI        
FseI        FspI        HindIII     HinfI       HpaI        
HphI        KpnI        KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacII       SbfI        ScaI        
SexAI       SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          625  GTCGAC               GT^[AC][GT]AC
AccII         123  CGCG                 CG^CG
AlwI          588  GATCC                GGATC(4/5)
AocI           37  CCTAAGG              CC^T[ACGT]AGG
AvaI          229  CCCGAG               C^[CT]CG[GA]G
BbeI           26  GGCGCC               GGCGC^C
BbsI          400  GAAGAC               GAAGAC(2/6)
BbvCI         564  CCTCAGC              CCTCAGC(-5/-2)
BglI          538  GCCCTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         362  AGATCT               A^GATCT
BpuAI         400  GAAGAC               GAAGAC(2/6)
BsaI          275  GAGACC               GGTCTC(1/5)
BseRI         232  GAGGAG               GAGGAG(10/8)
BsmBI         439  GAGACG               CGTCTC(1/5)
BsoBI         229  CCCGAG               C^[CT]CG[GA]G
BspMI         249  GCAGGT               ACCTGC(4/8)
BsrDI         195  CATTGC               GCAATG(2/0)
BstBI         631  TTCGAA               TT^CGAA
BstUI         123  CGCG                 CG^CG
Bsu36I         37  CCTAAGG              CC^T[ACGT]AGG
DrdI          616  GACGGTCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          507  CGGCCG               [CT]^GGCC[GA]
EagI          507  CGGCCG               C^GGCCG
EclXI         507  CGGCCG               C^GGCCG
Eco52I        507  CGGCCG               C^GGCCG
Eco81I         37  CCTAAGG              CC^T[ACGT]AGG
HincII        625  GTCGAC               GT[CT]^[GA]AC
HindII        625  GTCGAC               GT[CT]^[GA]AC
KasI           26  GGCGCC               G^GCGCC
MaeII         599  ACGT                 A^CGT
MspA1I        571  CAGCGG               C[AC]G^C[GT]G
MvnI          123  CGCG                 CG^CG
NarI           26  GGCGCC               GG^CGCC
NspV          631  TTCGAA               TT^CGAA
PflMI         594  CCAGGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          247  CTGCAG               CTGCA^G
SalI          625  GTCGAC               G^TCGAC
SapI          241  GAAGAGC              GCTCTTC(1/4)
SfcI          247  CTGCAG               C^T[GA][CT]AG
SfiI          537  GGCCCTCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           26  GGCGCC               GGC^GCC
SfuI          631  TTCGAA               TT^CGAA
SmlI          541  CTCAAG               C^T[CT][GA]AG
SphI          317  GCATGC               GCATG^C
Van91I        594  CCAGGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

ApaI           33  GGGCCC               GGGCC^C
              420  GGGCCC              
BanI           26  GGCGCC               G^G[CT][GA]CC
              310  GGTGCC              
BpmI           96  CTCCAG               CTGGAG(16/14)
              592  CTCCAG              
BsiEI         213  CGACCG               CG[GA][CT]^CG
              507  CGGCCG              
BstXI         341  CCACCGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              425  CCAAGCTGCTGG        
BstYI         362  AGATCT               [GA]^GATC[CT]
              587  AGATCC              
DdeI           38  CTAAG                C^T[ACGT]AG
              565  CTCAG               
EarI          241  GAAGAG               CTCTTC(1/4)
              634  GAAGAG              
EcoT14I        70  CCAAGG               C^C[AT][AT]GG
              104  CCAAGG              
FauI          121  CCCGC                CCCGC(4/6)
              573  GCGGG               
HaeII          26  GGCGCC               [GA]GCGC^[CT]
              560  AGCGCC              
HgaI          475  GACGC                GACGC(5/10)
              131  GCGTC               
Ksp632I       241  GAAGAG               CTCTTC(1/4)
              634  GAAGAG              
MflI          362  AGATCT               [GA]^GATC[CT]
              587  AGATCC              
MslI          342  CACCGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              513  CATCAGCGTG          
NspI          317  GCATGC               [GA]CATG^[CT]
              335  ACATGC              
PpuMI          41  AGGACCC              [GA]G^G[AT]CC[CT]
              116  GGGACCC             
SacI          352  GAGCTC               GAGCT^C
              526  GAGCTC              
SfaNI         176  GCATC                GCATC(5/9)
              512  GCATC               
StyI           70  CCAAGG               C^C[AT][AT]GG
              104  CCAAGG              
TspRI          52  CAGTG                CA[GC]TG[ACGT][ACGT]^
              378  CAGTG               
XhoII         362  AGATCT               [GA]^GATC[CT]
              587  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AcyI        AluI        AspHI       AvaII       
BanII       BbvI        BcnI        BmyI        BsaHI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       ItaI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       TaqI        TseI        TthHB8I     
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