Restriction map for pF1KE0264
 (Linear) (Six-base) MAPSORT of: KIEE0264.seq  Check: 4909  from: 1  to: 1245



KIEE0264.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     714    1245 
   Size:        714     531
 
AccI GT'mk_AC

Cuts at:      0     903    1245 
   Size:        903     342
 
AflII C'TTAA_G

Cuts at:      0     759    1245 
   Size:        759     486
 
AflIII A'CryG_T

Cuts at:      0      94    1245 
   Size:         94    1151
 
AleI CACnn'nnGTG

Cuts at:      0     541    1245 
   Size:        541     704
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1186    1245 
   Size:       1186      59
 
ApoI r'AATT_y

Cuts at:      0    1035    1245 
   Size:       1035     210
 
AvaI C'yCGr_G

Cuts at:      0     238    1245 
   Size:        238    1007
 
BanI G'GyrC_C

Cuts at:      0      21    1245 
   Size:         21    1224
 
BanII G_rGCy'C

Cuts at:      0     342    1245 
   Size:        342     903
 
BbeI G_GCGC'C

Cuts at:      0      25    1245 
   Size:         25    1220
 
Bme1580I G_kGCm'C

Cuts at:      0    1102    1245 
   Size:       1102     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     170     356     940    1245 
   Size:        134      36     186     584     305

  Fragments arranged by size: 

                 584     305     186     134      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      75    1245 
   Size:         75    1170
 
BsaHI Gr'CG_yC

Cuts at:      0      22    1245 
   Size:         22    1223
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     329     359    1245 
   Size:        329      30     886

  Fragments arranged by size: 

                 886     329      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1096    1245 
   Size:       1096     149
 
BseYI C'CCAG_C

Cuts at:      0      53    1245 
   Size:         53    1192
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     692    1245 
   Size:        391     301     553

  Fragments arranged by size: 

                 553     391     301
 
BsmI GAATG_Cn'

Cuts at:      0     104    1245 
   Size:        104    1141
 
Bsp1286I G_dGCh'C

Cuts at:      0     342    1102    1245 
   Size:        342     760     143

  Fragments arranged by size: 

                 760     342     143
 
BspHI T'CATG_A

Cuts at:      0    1187    1245 
   Size:       1187      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     714    1245 
   Size:        714     531
 
BsrDI GCAATG_nn'

Cuts at:      0     408    1245 
   Size:        408     837
 
BsrFI r'CCGG_y

Cuts at:      0     955    1245 
   Size:        955     290
 
BsrGI T'GTAC_A

Cuts at:      0     278    1245 
   Size:        278     967
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1097    1245 
   Size:       1097     148
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     295     307    1245 
   Size:        295      12     938

  Fragments arranged by size: 

                 938     295      12
 
BtgI C'CryG_G

Cuts at:      0     860     878    1245 
   Size:        860      18     367

  Fragments arranged by size: 

                 860     367      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     998    1245 
   Size:        998     247
 
BtsI GCAGTG_nn'

Cuts at:      0     141    1102    1245 
   Size:        141     961     143

  Fragments arranged by size: 

                 961     143     141
 
DraI TTT'AAA

Cuts at:      0     495     869    1245 
   Size:        495     374     376

  Fragments arranged by size: 

                 495     376     374
 
EaeI y'GGCC_r

Cuts at:      0     285     297     309     413     508    1245 
   Size:        285      12      12     104      95     737

  Fragments arranged by size: 

                 737     285     104      95      12      12
 
EarI CTCTTCn'nnn_

Cuts at:      0    1107    1245 
   Size:       1107     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     170     356     940    1245 
   Size:        134      36     186     584     305

  Fragments arranged by size: 

                 584     305     186     134      36
 
HaeII r_GCGC'y

Cuts at:      0      25    1245 
   Size:         25    1220
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     900     932    1151    1183    1191    1223    1245 
   Size:        900      32     219      32       8      32      22

  Fragments arranged by size: 

                 900     219      32      32      32      22       8
 
HincII GTy'rAC

Cuts at:      0     663     892    1245 
   Size:        663     229     353

  Fragments arranged by size: 

                 663     353     229
 
KasI G'GCGC_C

Cuts at:      0      21    1245 
   Size:         21    1224
 
MfeI C'AATT_G

Cuts at:      0     713    1245 
   Size:        713     532
 
MscI TGG'CCA

Cuts at:      0     287     299     415     510    1245 
   Size:        287      12     116      95     735

  Fragments arranged by size: 

                 735     287     116      95      12
 
MslI CAynn'nnrTG

Cuts at:      0     293     415     541    1132    1245 
   Size:        293     122     126     591     113

  Fragments arranged by size: 

                 591     293     126     122     113
 
MspA1I CmG'CkG

Cuts at:      0     397     427    1245 
   Size:        397      30     818

  Fragments arranged by size: 

                 818     397      30
 
NaeI GCC'GGC

Cuts at:      0     957    1245 
   Size:        957     288
 
NarI GG'CG_CC

Cuts at:      0      22    1245 
   Size:         22    1223
 
NgoMIV G'CCGG_C

Cuts at:      0     955    1245 
   Size:        955     290
 
NruI TCG'CGA

Cuts at:      0     983    1245 
   Size:        983     262
 
NspI r_CATG'y

Cuts at:      0      98    1245 
   Size:         98    1147
 
PciI A'CATG_T

Cuts at:      0      94    1245 
   Size:         94    1151
 
PfoI T'CCnGG_A

Cuts at:      0     147    1217    1245 
   Size:        147    1070      28

  Fragments arranged by size: 

                1070     147      28
 
PshAI GACnn'nnGTC

Cuts at:      0    1084    1245 
   Size:       1084     161
 
PstI C_TGCA'G

Cuts at:      0     531     774    1245 
   Size:        531     243     471

  Fragments arranged by size: 

                 531     471     243
 
PvuII CAG'CTG

Cuts at:      0     397    1245 
   Size:        397     848
 
SbfI CC_TGCA'GG

Cuts at:      0     531    1245 
   Size:        531     714
 
SfcI C'TryA_G

Cuts at:      0     527     770     824    1245 
   Size:        527     243      54     421

  Fragments arranged by size: 

                 527     421     243      54
 
SfoI GGC'GCC

Cuts at:      0      23    1245 
   Size:         23    1222
 
SmlI C'TyrA_G

Cuts at:      0     238     759    1245 
   Size:        238     521     486

  Fragments arranged by size: 

                 521     486     238
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      67    1245 
   Size:         67    1178
 
TatI w'GTAC_w

Cuts at:      0     175     278    1245 
   Size:        175     103     967

  Fragments arranged by size: 

                 967     175     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     329     361    1245 
   Size:        329      32     884

  Fragments arranged by size: 

                 884     329      32
 
XhoI C'TCGA_G

Cuts at:      0     238    1245 
   Size:        238    1007
 
XmnI GAAnn'nnTTC

Cuts at:      0     143    1245 
   Size:        143    1102
 

 Enzymes that do cut:

     AarI     AccI    AflII   AflIII     AleI     AlfI     ApoI     AvaI
     BanI    BanII     BbeI Bme1580I     BpmI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BstAPI    BstXI     BtgI    BtgZI     BtsI     DraI
     EaeI     EarI  Eco57MI    HaeII    Hin4I   HincII     KasI     MfeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NruI     NspI
     PciI     PfoI    PshAI     PstI    PvuII     SbfI     SfcI     SfoI
     SmlI    TaqII     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaWI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BsrBI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MluI     MmeI     NcoI     NdeI     NheI     NotI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XcmI     XmaI     ZraI
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