Restriction map for pF1KE0263
##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaJI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TaqI        
Tru9I       Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   32 enzymes) #
###################################

AspHI         246  GAGCAC               G[AT]GC[AT]^C
BanII           7  GAGCCC               G[GA]GC[CT]^C
BbvI           85  GCAGC                GCAGC(8/12)
BcnI           89  CCCGG                CC^[GC]GG
BglII         185  AGATCT               A^GATCT
BpmI           36  CTCCAG               CTGGAG(16/14)
BsaI           33  GGTCTC               GGTCTC(1/5)
BsaWI         233  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       246  GAGCAC               G[AT]GC[AT]^C
BstF5I        330  GGATG                GGATG(2/0)
BstYI         185  AGATCT               [GA]^GATC[CT]
DdeI          182  CTAAG                C^T[ACGT]AG
DpnII         186  GATC                 ^GATC
Fnu4HI         85  GCAGC                GC^[ACGT]GC
FokI          330  GGATG                GGATG(9/13)
ItaI           85  GCAGC                GC^[ACGT]GC
MaeII         281  ACGT                 A^CGT
MaeIII        121  GTAAC                ^GT[ACGT]AC
MboI          186  GATC                 ^GATC
MboII         205  GAAGA                GAAGA(8/7)
MflI          185  AGATCT               [GA]^GATC[CT]
MnlI           52  GAGG                 CCTC(7/6)
NciI           89  CCCGG                CC^[GC]GG
NdeII         186  GATC                 ^GATC
NlaIV          19  GGAACC               GG[ACGT]^[ACGT]CC
Sau3AI        186  GATC                 ^GATC
SfcI          221  CTACAG               C^T[GA][CT]AG
SspI          364  AATATT               AAT^ATT
TseI           85  GCAGC                G^C[AT]GC
TspRI          39  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          285  CCAAAAATGGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         185  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI           78  GAATTT               [GA]^AATT[CT]
              208  GAATTT              
AluI           29  AGCT                 AG^CT
              197  AGCT                
ApoI           78  GAATTT               [GA]^AATT[CT]
              208  GAATTT              
BmyI            7  GAGCCC               G[AGT]GC[ACT]^C
              246  GAGCAC              
BsiYI          10  CCCTTCTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               90  CCGGAACATGG         
BslI           10  CCCTTCTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               90  CCGGAACATGG         
BsmAI         304  GAGAC                GTCTC(1/5)
               34  GTCTC               
Bsp1286I        7  GAGCCC               G[AGT]GC[ACT]^C
              246  GAGCAC              
BsrI           69  ACTGG                ACTGG(1/-1)
               38  CCAGT               
BstNI          23  CCTGG                CC^[AT]GG
              174  CCAGG               
EcoRII         23  CCTGG                ^CC[AT]GG
              174  CCAGG               
HapII          90  CCGG                 C^CGG
              234  CCGG                
HinfI         276  GATTC                G^A[ACGT]TC
              380  GAATC               
HpaII          90  CCGG                 C^CGG
              234  CCGG                
MspI           90  CCGG                 C^CGG
              234  CCGG                
MvaI           23  CCTGG                CC^[AT]GG
              174  CCAGG               
NlaIII         60  CATG                 CATG^
               96  CATG                
TfiI          276  GATTC                G^A[AT]TC
              380  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

BssKI       ScrFI       Tsp509I     
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