Restriction map for pF1KE0253
 (Linear) (Six-base) MAPSORT of: KIEE0253.seq  Check: 5046  from: 1  to: 1176



KIEE0253.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

Acc65I G'GTAC_C

Cuts at:      0     581    1176 
   Size:        581     595
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     505    1176 
   Size:        198     307     671

  Fragments arranged by size: 

                 671     307     198
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     967    1176 
   Size:        967     209
 
AlwNI CAG_nnn'CTG

Cuts at:      0     248    1176 
   Size:        248     928
 
ApoI r'AATT_y

Cuts at:      0     562     715    1176 
   Size:        562     153     461

  Fragments arranged by size: 

                 562     461     153
 
BanI G'GyrC_C

Cuts at:      0     581    1176 
   Size:        581     595
 
BanII G_rGCy'C

Cuts at:      0     133    1019    1176 
   Size:        133     886     157

  Fragments arranged by size: 

                 886     157     133
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     693    1176 
   Size:        693     483
 
BbvCI CC'TCA_GC

Cuts at:      0     406     940    1176 
   Size:        406     534     236

  Fragments arranged by size: 

                 534     406     236
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1053    1176 
   Size:       1053     123
 
BclI T'GATC_A

Cuts at:      0     773    1176 
   Size:        773     403
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     200    1176 
   Size:        200     976
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     192    1038    1176 
   Size:        192     846     138

  Fragments arranged by size: 

                 846     192     138
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     807    1176 
   Size:        553     254     369

  Fragments arranged by size: 

                 553     369     254
 
Bpu10I CC'TnA_GC

Cuts at:      0     406     940    1176 
   Size:        406     534     236

  Fragments arranged by size: 

                 534     406     236
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     188     748    1176 
   Size:        188     560     428

  Fragments arranged by size: 

                 560     428     188
 
BsaAI yAC'GTr

Cuts at:      0     237    1176 
   Size:        237     939
 
BsaWI w'CCGG_w

Cuts at:      0     104    1176 
   Size:        104    1072
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     165     928    1176 
   Size:        165     763     248

  Fragments arranged by size: 

                 763     248     165
 
BseYI C'CCAG_C

Cuts at:      0     251     924    1176 
   Size:        251     673     252

  Fragments arranged by size: 

                 673     252     251
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493    1176 
   Size:        493     683
 
BsiHKAI G_wGCw'C

Cuts at:      0    1019    1176 
   Size:       1019     157
 
BsmI GAATG_Cn'

Cuts at:      0     157    1176 
   Size:        157    1019
 
Bsp1286I G_dGCh'C

Cuts at:      0     133    1019    1176 
   Size:        133     886     157

  Fragments arranged by size: 

                 886     157     133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     374    1176 
   Size:        374     802
 
BsrBI CCG'CTC

Cuts at:      0     579    1176 
   Size:        579     597
 
BsrFI r'CCGG_y

Cuts at:      0     369    1176 
   Size:        369     807
 
BssSI C'ACGA_G

Cuts at:      0     469    1176 
   Size:        469     707
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     480    1176 
   Size:        480     696
 
BstYI r'GATC_y

Cuts at:      0     488    1176 
   Size:        488     688
 
Bsu36I CC'TnA_GG

Cuts at:      0     631    1176 
   Size:        631     545
 
BtsI GCAGTG_nn'

Cuts at:      0     259    1176 
   Size:        259     917
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     109     795    1176 
   Size:        109     686     381

  Fragments arranged by size: 

                 686     381     109
 
EaeI y'GGCC_r

Cuts at:      0     288    1176 
   Size:        288     888
 
EarI CTCTTCn'nnn_

Cuts at:      0     493    1176 
   Size:        493     683
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     454     651    1176 
   Size:        454     197     525

  Fragments arranged by size: 

                 525     454     197
 
EcoICRI GAG'CTC

Cuts at:      0    1017    1176 
   Size:       1017     159
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     505     553     807    1176 
   Size:        198     307      48     254     369

  Fragments arranged by size: 

                 369     307     254     198      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0      64     324    1176 
   Size:         64     260     852

  Fragments arranged by size: 

                 852     260      64
 
EcoRI G'AATT_C

Cuts at:      0     562     715    1176 
   Size:        562     153     461

  Fragments arranged by size: 

                 562     461     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      56      88     950     982     992    1024    1176 
   Size:         56      32     862      32      10      32     152

  Fragments arranged by size: 

                 862     152      56      32      32      32      10
 
KpnI G_GTAC'C

Cuts at:      0     585    1176 
   Size:        585     591
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     530    1176 
   Size:        530     646
 
MscI TGG'CCA

Cuts at:      0     290    1176 
   Size:        290     886
 
MslI CAynn'nnrTG

Cuts at:      0     695     879    1176 
   Size:        695     184     297

  Fragments arranged by size: 

                 695     297     184
 
MspA1I CmG'CkG

Cuts at:      0      19     194     394     722    1176 
   Size:         19     175     200     328     454

  Fragments arranged by size: 

                 454     328     200     175      19
 
NspI r_CATG'y

Cuts at:      0      33     483     593     782    1163    1176 
   Size:         33     450     110     189     381      13

  Fragments arranged by size: 

                 450     381     189     110      33      13
 
PfoI T'CCnGG_A

Cuts at:      0     707    1176 
   Size:        707     469
 
PpuMI rG'GwC_Cy

Cuts at:      0      64    1176 
   Size:         64    1112
 
PshAI GACnn'nnGTC

Cuts at:      0     760    1176 
   Size:        760     416
 
PsiI TTA'TAA

Cuts at:      0     452    1176 
   Size:        452     724
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     585     617    1176 
   Size:        585      32     559

  Fragments arranged by size: 

                 585     559      32
 
PstI C_TGCA'G

Cuts at:      0      24    1176 
   Size:         24    1152
 
PvuII CAG'CTG

Cuts at:      0      19     194     394     722    1176 
   Size:         19     175     200     328     454

  Fragments arranged by size: 

                 454     328     200     175      19
 
SacI G_AGCT'C

Cuts at:      0    1019    1176 
   Size:       1019     157
 
SanDI GG'GwC_CC

Cuts at:      0      64    1176 
   Size:         64    1112
 
SfcI C'TryA_G

Cuts at:      0      20    1176 
   Size:         20    1156
 
SspI AAT'ATT

Cuts at:      0    1083    1176 
   Size:       1083      93
 
StyI C'CwwG_G

Cuts at:      0     678    1176 
   Size:        678     498
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1084    1176 
   Size:       1084      92
 
TatI w'GTAC_w

Cuts at:      0      26    1176 
   Size:         26    1150
 
Tth111I GACn'n_nGTC

Cuts at:      0      83    1176 
   Size:         83    1093
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI    AlwNI     ApoI     BanI    BanII     BbsI
    BbvCI    BciVI     BclI     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstYI   Bsu36I     BtsI
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     NspI     PfoI
    PpuMI    PshAI     PsiI     PsrI     PstI    PvuII     SacI    SanDI
     SfcI     SspI     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BcgI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI    BpuEI    BsaBI    BsaHI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI   PspOMI    PspXI     PvuI    RsrII    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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