Restriction map for pF1KE0250
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AflIII      AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     FauI        FbaI        FseI        
FspI        HaeIII      HgaI        Hin1I       HincII      
HindII      HinfI       HpaI        KasI        KpnI        
MaeI        MamI        MfeI        MluI        MluNI       
MscI        MseI        MslI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NspI        NspV        PacI        PaeR7I      
PflFI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccIII        575  TCCGGA               T^CCGGA
AfaI          436  GTAC                 GT^AC
AgeI           98  ACCGGT               A^CCGGT
BamHI         578  GGATCC               G^GATCC
BcnI          105  CCGGG                CC^[GC]GG
BglII         389  AGATCT               A^GATCT
BmyI          107  GGGCAC               G[AGT]GC[ACT]^C
BseAI         575  TCCGGA               T^CCGGA
BsmFI          41  GGGAC                GGGAC(10/14)
Bsp1286I      107  GGGCAC               G[AGT]GC[ACT]^C
BspEI         575  TCCGGA               T^CCGGA
BspMI         411  GCAGGT               ACCTGC(4/8)
BsrI          111  ACTGG                ACTGG(1/-1)
BstAPI        399  GCATGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI           23  CCGCGG               C^C[GA][CT]GG
EarI          448  GAAGAG               CTCTTC(1/4)
EcoT22I       353  ATGCAT               ATGCA^T
HaeII         165  GGCGCT               [GA]GCGC^[CT]
HindIII       204  AAGCTT               A^AGCTT
Ksp632I       448  GAAGAG               CTCTTC(1/4)
KspI           23  CCGCGG               CCGC^GG
MaeII         554  ACGT                 A^CGT
MaeIII        442  GTGAC                ^GT[ACGT]AC
MroI          575  TCCGGA               T^CCGGA
NciI          105  CCGGG                CC^[GC]GG
NruI          378  TCGCGA               TCG^CGA
NsiI          353  ATGCAT               ATGCA^T
PflMI         225  CCAGCGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI          98  ACCGGT               A^CCGGT
PstI          409  CTGCAG               CTGCA^G
PvuII         454  CAGCTG               CAG^CTG
RsaI          436  GTAC                 GT^AC
SacII          23  CCGCGG               CCGC^GG
SapI          448  GAAGAGC              GCTCTTC(1/4)
Tsp45I        442  GTGAC                ^GT[GC]AC
Tsp509I       195  AATT                 ^AATT
TspRI         110  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        225  CCAGCGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BsaI           58  GAGACC               GGTCTC(1/5)
              188  GAGACC              
BsaWI          98  ACCGGT               [AT]^CCGG[AT]
              575  TCCGGA              
BsgI           91  CTGCAC               GTGCAG(16/14)
              481  GTGCAG              
BsmAI          58  GAGAC                GTCTC(1/5)
              188  GAGAC               
BsrFI          61  ACCGGC               [GA]^CCGG[CT]
               98  ACCGGT              
BstF5I        573  CATCC                GGATG(2/0)
              504  GGATG               
CfoI            9  GCGC                 GCG^C
              166  GCGC                
Cfr10I         61  ACCGGC               [GA]^CCGG[CT]
               98  ACCGGT              
DdeI          136  CTGAG                C^T[ACGT]AG
              540  CTTAG               
FokI          573  CATCC                GGATG(9/13)
              504  GGATG               
HhaI            9  GCGC                 GCG^C
              166  GCGC                
HinP1I          9  GCGC                 G^CGC
              166  GCGC                
HphI          441  GGTGA                GGTGA(8/7)
              530  TCACC               
MspA1I         23  CCGCGG               C[AC]G^C[GT]G
              454  CAGCTG              
NlaIII        144  CATG                 CATG^
              400  CATG                
SfaNI         405  GATGC                GCATC(5/9)
              572  GCATC               
SfcI          275  CTGTAG               C^T[GA][CT]AG
              409  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BbvI        
BpmI        BsaJI       BsiYI       BslI        BssKI       
BstNI       BstUI       BstYI       Cac8I       DpnII       
EcoRII      Fnu4HI      HapII       HpaII       ItaI        
MboI        MboII       MflI        MnlI        MspI        
MvaI        MvnI        MwoI        NdeII       NlaIV       
Sau3AI      ScrFI       TseI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com