Restriction map for pF1KE0244
##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        TfiI        TseI        Tth111I     
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI          196  GAATTT               [GA]^AATT[CT]
AluI          370  AGCT                 AG^CT
ApoI          196  GAATTT               [GA]^AATT[CT]
BanII         270  GGGCTC               G[GA]GC[CT]^C
BmyI          270  GGGCTC               G[AGT]GC[ACT]^C
BsaAI          58  CACGTA               [CT]AC^GT[GA]
BsaJI         341  CCTTGG               C^C[ACGT][ACGT]GG
BsiYI          57  CCACGTATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           57  CCACGTATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      270  GGGCTC               G[AGT]GC[ACT]^C
Bsp1407I      291  TGTACA               T^GTACA
BspMI          52  ACCTGC               ACCTGC(4/8)
BsrGI         291  TGTACA               T^GTACA
BsrI           84  ACTGG                ACTGG(1/-1)
BssKI         388  CCAGG                ^CC[ACGT]GG
BstBI          23  TTCGAA               TT^CGAA
BstF5I        111  CATCC                GGATG(2/0)
BstNI         388  CCAGG                CC^[AT]GG
CfoI          186  GCGC                 GCG^C
EcoRII        388  CCAGG                ^CC[AT]GG
EcoT14I       341  CCTTGG               C^C[AT][AT]GG
FokI          111  CATCC                GGATG(9/13)
HhaI          186  GCGC                 GCG^C
HinP1I        186  GCGC                 G^CGC
HphI           41  TCACC                GGTGA(8/7)
MaeIII         40  GTCAC                ^GT[ACGT]AC
MvaI          388  CCAGG                CC^[AT]GG
MwoI          177  GCTACCTGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        282  CATG                 CATG^
NspV           23  TTCGAA               TT^CGAA
PshAI         144  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScrFI         388  CCAGG                CC^[ACGT]GG
SfuI           23  TTCGAA               TT^CGAA
SmlI          265  CTTGAG               C^T[CT][GA]AG
SspBI         291  TGTACA               T^GTACA
SspI          208  AATATT               AAT^ATT
StyI          341  CCTTGG               C^C[AT][AT]GG
Tsp45I         40  GTCAC                ^GT[GC]AC
Tsp509I       197  AATT                 ^AATT
TspRI         360  CACTG                CA[GC]TG[ACGT][ACGT]^
XbaI          397  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AfaI          292  GTAC                 GT^AC
              320  GTAC                
AvaII         143  GGACC                G^G[AT]CC
              385  GGTCC               
BclI           93  TGATCA               T^GATCA
              356  TGATCA              
BfaI          314  CTAG                 C^TAG
              398  CTAG                
BsmFI          76  GGGAC                GGGAC(10/14)
              142  GGGAC               
Cac8I         226  GCCTGC               GC[ACGT]^[ACGT]GC
              297  GCCTGC              
Cfr13I        143  GGACC                G^G[ACGT]CC
              385  GGTCC               
DpnII          94  GATC                 ^GATC
              357  GATC                
FbaI           93  TGATCA               T^GATCA
              356  TGATCA              
HinfI          78  GACTC                G^A[ACGT]TC
              309  GACTC               
MaeI          314  CTAG                 C^TAG
              398  CTAG                
MboI           94  GATC                 ^GATC
              357  GATC                
MseI           32  TTAA                 T^TAA
              212  TTAA                
NdeII          94  GATC                 ^GATC
              357  GATC                
NlaIV         142  GGGACC               GG[ACGT]^[ACGT]CC
              271  GGCTCC              
PleI           78  GACTC                GAGTC(4/5)
              309  GACTC               
RsaI          292  GTAC                 GT^AC
              320  GTAC                
Sau3AI         94  GATC                 ^GATC
              357  GATC                
Sau96I        143  GGACC                G^G[ACGT]CC
              385  GGTCC               
TaqI           24  TCGA                 T^CGA
               81  TCGA                
Tru9I          32  TTAA                 T^TAA
              212  TTAA                
TthHB8I        24  TCGA                 T^CGA
               81  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MaeII       MboII       MnlI        
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