Restriction map for pF1KE0244
 (Linear) (Six-base) MAPSORT of: KIEE0244.seq  Check: 8909  from: 1  to: 402



KIEE0244.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

ApoI r'AATT_y

Cuts at:      0     196     402 
   Size:        196     206
 
BanII G_rGCy'C

Cuts at:      0     274     402 
   Size:        274     128
 
BclI T'GATC_A

Cuts at:      0      93     356     402 
   Size:         93     263      46

  Fragments arranged by size: 

                 263      93      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     402 
   Size:        286     116
 
BsaAI yAC'GTr

Cuts at:      0      60     402 
   Size:         60     342
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     322     352     402 
   Size:        322      30      50

  Fragments arranged by size: 

                 322      50      30
 
BseYI C'CCAG_C

Cuts at:      0     276     402 
   Size:        276     126
 
Bsp1286I G_dGCh'C

Cuts at:      0     274     402 
   Size:        274     128
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      61     402 
   Size:         61     341
 
BsrGI T'GTAC_A

Cuts at:      0     291     402 
   Size:        291     111
 
BstBI TT'CG_AA

Cuts at:      0      24     402 
   Size:         24     378
 
PshAI GACnn'nnGTC

Cuts at:      0     148     402 
   Size:        148     254
 
SmlI C'TyrA_G

Cuts at:      0     265     402 
   Size:        265     137
 
SspI AAT'ATT

Cuts at:      0     210     402 
   Size:        210     192
 
StyI C'CwwG_G

Cuts at:      0     341     402 
   Size:        341      61
 
TatI w'GTAC_w

Cuts at:      0     291     402 
   Size:        291     111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     322     354     402 
   Size:        322      32      48

  Fragments arranged by size: 

                 322      48      32
 
XbaI T'CTAG_A

Cuts at:      0     397     402 
   Size:        397       5
 

 Enzymes that do cut:

     ApoI    BanII     BclI    BpuEI    BsaAI    BsaXI    BseYI Bsp1286I
    BspMI    BsrGI    BstBI    PshAI     SmlI     SspI     StyI     TatI
     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
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