Restriction map for pF1KE0240
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      ApaLI       AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MaeII       MamI        
MfeI        MluI        MroI        MseI        MslI        
MunI        MvnI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Acc65I         40  GGTACC               G^GTACC
AcsI          106  GAATTT               [GA]^AATT[CT]
AocI         1210  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1028  AGCGCT               AGC^GCT
ApoI          106  GAATTT               [GA]^AATT[CT]
Asp700I       864  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        40  GGTACC               G^GTACC
BalI          731  TGGCCA               TGG^CCA
BbsI          877  GAAGAC               GAAGAC(2/6)
BciVI        1227  GGATAC               GTATCC(6/5)
BglI          448  GCCACCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         877  GAAGAC               GAAGAC(2/6)
BsrFI         961  GCCGGC               [GA]^CCGG[CT]
BstAPI       1142  GCAGTCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1210  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        961  GCCGGC               [GA]^CCGG[CT]
EaeI          731  TGGCCA               [CT]^GGCC[GA]
EarI         1324  CTCTTC               CTCTTC(1/4)
Eco47III     1028  AGCGCT               AGC^GCT
Eco81I       1210  CCTCAGG              CC^T[ACGT]AGG
HaeII        1028  AGCGCT               [GA]GCGC^[CT]
KpnI           40  GGTACC               GGTAC^C
Ksp632I      1324  CTCTTC               CTCTTC(1/4)
MluNI         731  TGGCCA               TGG^CCA
MscI          731  TGGCCA               TGG^CCA
NaeI          961  GCCGGC               GCC^GGC
NgoMIV        961  GCCGGC               G^CCGGC
TaqI         1013  TCGA                 T^CGA
TfiI         1059  GAATC                G^A[AT]TC
TthHB8I      1013  TCGA                 T^CGA
XmnI          864  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AlwNI         434  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1124  CAGCCCCTG           
AspHI         188  GTGCTC               G[AT]GC[AT]^C
             1298  GTGCTC              
AvaI          328  CCCGGG               C^[CT]CG[GA]G
              607  CCCGGG              
BanI           40  GGTACC               G^G[CT][GA]CC
              661  GGTGCC              
BfaI          517  CTAG                 C^TAG
              859  CTAG                
BlpI          353  GCTCAGC              GC^T[ACGT]AGC
             1031  GCTCAGC             
Bpu1102I      353  GCTCAGC              GC^T[ACGT]AGC
             1031  GCTCAGC             
BsaI          196  GAGACC               GGTCTC(1/5)
              773  GAGACC              
BsaWI         128  ACCGGA               [AT]^CCGG[AT]
              173  TCCGGT              
BsgI          402  CTGCAC               GTGCAG(16/14)
              177  GTGCAG              
BsiHKAI       188  GTGCTC               G[AT]GC[AT]^C
             1298  GTGCTC              
BsoBI         328  CCCGGG               C^[CT]CG[GA]G
              607  CCCGGG              
BspMI         802  ACCTGC               ACCTGC(4/8)
             1173  ACCTGC              
BstEII        930  GGTGACC              G^GT[ACGT]ACC
             1311  GGTGACC             
BstPI         930  GGTGACC              G^GT[ACGT]ACC
             1311  GGTGACC             
BstXI         294  CCAGAAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              632  CCATGGGTCTGG        
BstYI         131  GGATCT               [GA]^GATC[CT]
             1166  AGATCC              
CelII         353  GCTCAGC              GC^T[ACGT]AGC
             1031  GCTCAGC             
DsaI          632  CCATGG               C^C[GA][CT]GG
             1184  CCATGG              
EcoO65I       930  GGTGACC              G^GT[ACGT]ACC
             1311  GGTGACC             
MaeI          517  CTAG                 C^TAG
              859  CTAG                
MflI          131  GGATCT               [GA]^GATC[CT]
             1166  AGATCC              
NcoI          632  CCATGG               C^CATGG
             1184  CCATGG              
PflMI         918  CCAGACGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1266  CCAGACTTTGG         
PvuII         847  CAGCTG               CAG^CTG
             1160  CAGCTG              
SmaI          328  CCCGGG               CCC^GGG
              607  CCCGGG              
Van91I        918  CCAGACGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1266  CCAGACTTTGG         
XhoII         131  GGATCT               [GA]^GATC[CT]
             1166  AGATCC              
XmaCI         328  CCCGGG               C^CCGGG
              607  CCCGGG              
XmaI          328  CCCGGG               C^CCGGG
              607  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      Tsp509I     TspRI       
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