Restriction map for pF1KE0240
 (Linear) (Six-base) MAPSORT of: KIEE0240.seq  Check: 5488  from: 1  to: 1470



KIEE0240.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

Acc65I G'GTAC_C

Cuts at:      0      40    1470 
   Size:         40    1430
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1311    1470 
   Size:       1311     159
 
AfeI AGC'GCT

Cuts at:      0    1030    1470 
   Size:       1030     440
 
AlwNI CAG_nnn'CTG

Cuts at:      0     439    1129    1470 
   Size:        439     690     341

  Fragments arranged by size: 

                 690     439     341
 
ApaI G_GGCC'C

Cuts at:      0     722     791     998    1470 
   Size:        722      69     207     472

  Fragments arranged by size: 

                 722     472     207      69
 
ApoI r'AATT_y

Cuts at:      0     106    1470 
   Size:        106    1364
 
AvaI C'yCGr_G

Cuts at:      0     328     607    1470 
   Size:        328     279     863

  Fragments arranged by size: 

                 863     328     279
 
BanI G'GyrC_C

Cuts at:      0      40     661    1470 
   Size:         40     621     809

  Fragments arranged by size: 

                 809     621      40
 
BanII G_rGCy'C

Cuts at:      0     355     498     722     791     998    1244    1470 
   Size:        355     143     224      69     207     246     226

  Fragments arranged by size: 

                 355     246     226     224     207     143      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     884    1470 
   Size:        884     586
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1221    1470 
   Size:       1221     249
 
BglI GCCn_nnn'nGGC

Cuts at:      0     454    1470 
   Size:        454    1016
 
BlpI GC'TnA_GC

Cuts at:      0     354    1032    1470 
   Size:        354     678     438

  Fragments arranged by size: 

                 678     438     354
 
Bme1580I G_kGCm'C

Cuts at:      0     478     666     701     722     791     998    1184    1470 
   Size:        478     188      35      21      69     207     186     286

  Fragments arranged by size: 

                 478     286     207     188     186      69      35      21
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     556     688    1283    1470 
   Size:        556     132     595     187

  Fragments arranged by size: 

                 595     556     187     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     214     323     461     474     510     584     871 
   Size:         55     159     109     138      13      36      74     287

Cuts at:    871    1470 
   Size:        599

  Fragments arranged by size: 

                 599     287     159     138     109      74      55      36
                  13
 
Bpu10I CC'TnA_GC

Cuts at:      0     368    1470 
   Size:        368    1102
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     190     767    1470 
   Size:        190     577     703

  Fragments arranged by size: 

                 703     577     190
 
BsaWI w'CCGG_w

Cuts at:      0     128     173    1470 
   Size:        128      45    1297

  Fragments arranged by size: 

                1297     128      45
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     639     669    1470 
   Size:        639      30     801

  Fragments arranged by size: 

                 801     639      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     349     730     868    1470 
   Size:        349     381     138     602

  Fragments arranged by size: 

                 602     381     349     138
 
BseYI C'CCAG_C

Cuts at:      0     463     938    1470 
   Size:        463     475     532

  Fragments arranged by size: 

                 532     475     463
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     387    1470 
   Size:        198     189    1083

  Fragments arranged by size: 

                1083     198     189
 
BsiHKAI G_wGCw'C

Cuts at:      0     192    1302    1470 
   Size:        192    1110     168

  Fragments arranged by size: 

                1110     192     168
 
Bsp1286I G_dGCh'C

Cuts at:      0     192     355     478     498     666     701     722     791 
   Size:        192     163     123      20     168      35      21      69

Cuts at:    791     998    1184    1244    1302    1470 
   Size:        207     186      60      58     168

  Fragments arranged by size: 

                 207     192     186     168     168     163     123      69
                  60      58      35      21      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     811    1182    1470 
   Size:        811     371     288

  Fragments arranged by size: 

                 811     371     288
 
BsrBI CCG'CTC

Cuts at:      0     488     524     839    1470 
   Size:        488      36     315     631

  Fragments arranged by size: 

                 631     488     315      36
 
BsrFI r'CCGG_y

Cuts at:      0     961    1470 
   Size:        961     509
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1148    1470 
   Size:       1148     322
 
BstEII G'GTnAC_C

Cuts at:      0     930    1311    1470 
   Size:        930     381     159

  Fragments arranged by size: 

                 930     381     159
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     301     639    1470 
   Size:        301     338     831

  Fragments arranged by size: 

                 831     338     301
 
BstYI r'GATC_y

Cuts at:      0     131    1166    1470 
   Size:        131    1035     304

  Fragments arranged by size: 

                1035     304     131
 
Bsu36I CC'TnA_GG

Cuts at:      0    1211    1470 
   Size:       1211     259
 
BtgI C'CryG_G

Cuts at:      0     632    1184    1470 
   Size:        632     552     286

  Fragments arranged by size: 

                 632     552     286
 
BtsI GCAGTG_nn'

Cuts at:      0    1399    1428    1470 
   Size:       1399      29      42

  Fragments arranged by size: 

                1399      42      29
 
EaeI y'GGCC_r

Cuts at:      0     731    1470 
   Size:        731     739
 
EarI CTCTTCn'nnn_

Cuts at:      0    1330    1470 
   Size:       1330     140
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1004    1470 
   Size:       1004     466
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     214     323     461     474     510     584     871 
   Size:         55     159     109     138      13      36      74     287

Cuts at:    871    1311    1470 
   Size:        440     159

  Fragments arranged by size: 

                 440     287     159     159     138     109      74      55
                  36      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     325     530     590     718     719     787     788     994 
   Size:        325     205      60     128       1      68       1     206

Cuts at:    994    1454    1470 
   Size:        460      16

  Fragments arranged by size: 

                 460     325     206     205     128      68      60      16
                   1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      84    1470 
   Size:         84    1386
 
HaeII r_GCGC'y

Cuts at:      0    1032    1470 
   Size:       1032     438
 
KpnI G_GTAC'C

Cuts at:      0      44    1470 
   Size:         44    1426
 
MscI TGG'CCA

Cuts at:      0     733    1470 
   Size:        733     737
 
MspA1I CmG'CkG

Cuts at:      0     849    1036    1162    1470 
   Size:        849     187     126     308

  Fragments arranged by size: 

                 849     308     187     126
 
NaeI GCC'GGC

Cuts at:      0     963    1470 
   Size:        963     507
 
NcoI C'CATG_G

Cuts at:      0     632    1184    1470 
   Size:        632     552     286

  Fragments arranged by size: 

                 632     552     286
 
NgoMIV G'CCGG_C

Cuts at:      0     961    1470 
   Size:        961     509
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     924    1272    1470 
   Size:        924     348     198

  Fragments arranged by size: 

                 924     348     198
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     230     262    1470 
   Size:        230      32    1208

  Fragments arranged by size: 

                1208     230      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     325     590    1454    1470 
   Size:        325     265     864      16

  Fragments arranged by size: 

                 864     325     265      16
 
PspOMI G'GGCC_C

Cuts at:      0     718     787     994    1470 
   Size:        718      69     207     476

  Fragments arranged by size: 

                 718     476     207      69
 
PstI C_TGCA'G

Cuts at:      0     324    1125    1161    1470 
   Size:        324     801      36     309

  Fragments arranged by size: 

                 801     324     309      36
 
PvuII CAG'CTG

Cuts at:      0     849    1162    1470 
   Size:        849     313     308

  Fragments arranged by size: 

                 849     313     308
 
SfcI C'TryA_G

Cuts at:      0     320    1121    1157    1470 
   Size:        320     801      36     313

  Fragments arranged by size: 

                 801     320     313      36
 
SmaI CCC'GGG

Cuts at:      0     330     609    1470 
   Size:        330     279     861

  Fragments arranged by size: 

                 861     330     279
 
StyI C'CwwG_G

Cuts at:      0     632    1184    1368    1470 
   Size:        632     552     184     102

  Fragments arranged by size: 

                 632     552     184     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1035    1297    1470 
   Size:       1035     262     173

  Fragments arranged by size: 

                1035     262     173
 
TatI w'GTAC_w

Cuts at:      0     745    1470 
   Size:        745     725
 
XmaI C'CCGG_G

Cuts at:      0     328     607    1470 
   Size:        328     279     863

  Fragments arranged by size: 

                 863     328     279
 
XmnI GAAnn'nnTTC

Cuts at:      0     868    1470 
   Size:        868     602
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbsI    BciVI     BglI     BlpI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EarI     EciI  Eco57MI EcoO109I
     FalI    HaeII     KpnI     MscI   MspA1I     NaeI     NcoI   NgoMIV
    PflMI     PpiI    PpuMI   PspOMI     PstI    PvuII     SfcI     SmaI
     StyI    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     MslI     NarI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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