Restriction map for pF1KE0221
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SwaI        TaqI        Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I         81  GGTACC               G^GTACC
AciI          112  GCGG                 CCGC(-3/-1)
AfaI           82  GTAC                 GT^AC
AocI          129  CCTGAGG              CC^T[ACGT]AGG
Asp718I        81  GGTACC               G^GTACC
BanI           81  GGTACC               G^G[CT][GA]CC
BbvCI         261  GCTGAGG              CCTCAGC(-5/-2)
BbvI           33  GCTGC                GCAGC(8/12)
BfaI           37  CTAG                 C^TAG
BpmI           90  CTCCAG               CTGGAG(16/14)
BspMI          78  GCAGGT               ACCTGC(4/8)
BssKI          21  CCTGG                ^CC[ACGT]GG
BstNI          21  CCTGG                CC^[AT]GG
BstXI          14  CCACAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        129  CCTGAGG              CC^T[ACGT]AGG
CfoI          218  GCGC                 GCG^C
DpnII           6  GATC                 ^GATC
EaeI           49  TGGCCG               [CT]^GGCC[GA]
EarI          198  GAAGAG               CTCTTC(1/4)
Eco81I        129  CCTGAGG              CC^T[ACGT]AGG
EcoRII         21  CCTGG                ^CC[AT]GG
EcoT14I       221  CCTTGG               C^C[AT][AT]GG
Fnu4HI         33  GCTGC                GC^[ACGT]GC
HapII          52  CCGG                 C^CGG
HhaI          218  GCGC                 GCG^C
HinP1I        218  GCGC                 G^CGC
HinfI         123  GATTC                G^A[ACGT]TC
HpaII          52  CCGG                 C^CGG
HphI           11  TCACC                GGTGA(8/7)
ItaI           33  GCTGC                GC^[ACGT]GC
KpnI           81  GGTACC               GGTAC^C
Ksp632I       198  GAAGAG               CTCTTC(1/4)
MaeI           37  CTAG                 C^TAG
MaeIII        146  GTTAC                ^GT[ACGT]AC
MboI            6  GATC                 ^GATC
MseI          184  TTAA                 T^TAA
MspI           52  CCGG                 C^CGG
MvaI           21  CCTGG                CC^[AT]GG
MwoI          161  GCTCCATCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII           6  GATC                 ^GATC
NheI           36  GCTAGC               G^CTAGC
NlaIII         75  CATG                 CATG^
NlaIV          81  GGTACC               GG[ACGT]^[ACGT]CC
NspI           74  GCATGC               [GA]CATG^[CT]
RsaI           82  GTAC                 GT^AC
Sau3AI          6  GATC                 ^GATC
ScrFI          21  CCTGG                CC^[ACGT]GG
SphI           74  GCATGC               GCATG^C
SspI          186  AATATT               AAT^ATT
StuI          212  AGGCCT               AGG^CCT
StyI          221  CCTTGG               C^C[AT][AT]GG
TfiI          123  GATTC                G^A[AT]TC
Tru9I         184  TTAA                 T^TAA
TseI           33  GCTGC                G^C[AT]GC
TspRI         268  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BsaJI          20  CCCTGG               C^C[ACGT][ACGT]GG
              221  CCTTGG              
BstF5I         44  GGATG                GGATG(2/0)
              235  GGATG               
DdeI          130  CTGAG                C^T[ACGT]AG
              262  CTGAG               
FokI           44  GGATG                GGATG(9/13)
              235  GGATG               
HaeIII         50  GGCC                 GG^CC
              213  GGCC                
MboII          55  GAAGA                GAAGA(8/7)
              198  GAAGA               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AluI        Cac8I       MnlI        
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