Restriction map for pF1KE0220
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# NO CUT ENZYMES (  230 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MnlI        
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TseI        Tsp45I      TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   19 enzymes) #
###################################

AcsI           94  GAATTC               [GA]^AATT[CT]
ApoI           94  GAATTC               [GA]^AATT[CT]
AseI          181  ATTAAT               AT^TAAT
AsnI          181  ATTAAT               AT^TAAT
BglI           27  GCCAAGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI         110  CCATGG               C^C[ACGT][ACGT]GG
BstXI          53  CCATTCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI          110  CCATGG               C^C[GA][CT]GG
EcoRI          94  GAATTC               G^AATTC
EcoT14I       110  CCATGG               C^C[AT][AT]GG
MboII         102  GAAGA                GAAGA(8/7)
MwoI           27  GCCAAGTCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          110  CCATGG               C^CATGG
NlaIII        111  CATG                 CATG^
PleI           49  GACTC                GAGTC(4/5)
PshBI         181  ATTAAT               AT^TAAT
SfaNI          71  GATGC                GCATC(5/9)
StyI          110  CCATGG               C^C[AT][AT]GG
TfiI          143  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwI          168  GATCC                GGATC(4/5)
                3  GGATC               
DpnII           4  GATC                 ^GATC
              168  GATC                
HinfI          49  GACTC                G^A[ACGT]TC
              143  GATTC               
MboI            4  GATC                 ^GATC
              168  GATC                
MseI           65  TTAA                 T^TAA
              182  TTAA                
NdeII           4  GATC                 ^GATC
              168  GATC                
Sau3AI          4  GATC                 ^GATC
              168  GATC                
Tru9I          65  TTAA                 T^TAA
              182  TTAA                
Tsp509I        95  AATT                 ^AATT
              164  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################


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