Restriction map for pF1KE0220
 (Linear) (Six-base) MAPSORT of: KIEE0220.seq  Check: 1575  from: 1  to: 204



KIEE0220.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

ApoI r'AATT_y

Cuts at:      0      94     204 
   Size:         94     110
 
AseI AT'TA_AT

Cuts at:      0     182     204 
   Size:        182      22
 
BglI GCCn_nnn'nGGC

Cuts at:      0      33     204 
   Size:         33     171
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      60     204 
   Size:         60     144
 
BtgI C'CryG_G

Cuts at:      0     110     204 
   Size:        110      94
 
EcoRI G'AATT_C

Cuts at:      0      94     204 
   Size:         94     110
 
NcoI C'CATG_G

Cuts at:      0     110     204 
   Size:        110      94
 
StyI C'CwwG_G

Cuts at:      0     110     204 
   Size:        110      94
 

 Enzymes that do cut:

     ApoI     AseI     BglI    BstXI     BtgI    EcoRI     NcoI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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