Restriction map for pF1KE2076
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Aor51HI     AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BcgI        BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BspMI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CelII       
ClaI        CpoI        DraI        Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoRI       EcoT22I     FseI        
FspI        HaeII       HgaI        HindIII     KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
Van91I      XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        1652  GACGTC               GACGT^C
AccII          30  CGCG                 CG^CG
AcyI         1652  GACGTC               G[GA]^CG[CT]C
AocI         1867  CCTAAGG              CC^T[ACGT]AGG
ApaI           37  GGGCCC               GGGCC^C
AseI          906  ATTAAT               AT^TAAT
AsnI          906  ATTAAT               AT^TAAT
Asp700I       994  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           12  CCCGGG               C^[CT]CG[GA]G
BalI         2151  TGGCCA               TGG^CCA
BciVI        1582  GGATAC               GTATCC(6/5)
BglI            9  GCCCCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         511  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1652  GACGTC               G[GA]^CG[CT]C
BsiEI          22  CGGCCG               CG[GA][CT]^CG
BsmI         1457  GCATTC               GAATGC(1/-1)
BsoBI          12  CCCGGG               C^[CT]CG[GA]G
BsrFI          67  ACCGGC               [GA]^CCGG[CT]
BstUI          30  CGCG                 CG^CG
BstXI         679  CCATTTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1867  CCTAAGG              CC^T[ACGT]AGG
CfoI           29  GCGC                 GCG^C
Cfr10I         67  ACCGGC               [GA]^CCGG[CT]
DrdI         1906  GACAATACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           22  CGGCCG               C^GGCCG
EclXI          22  CGGCCG               C^GGCCG
Eco52I         22  CGGCCG               C^GGCCG
Eco81I       1867  CCTAAGG              CC^T[ACGT]AGG
EcoRV        1503  GATATC               GAT^ATC
HhaI           29  GCGC                 GCG^C
Hin1I        1652  GACGTC               G[GA]^CG[CT]C
HinP1I         29  GCGC                 G^CGC
HpaI         2226  GTTAAC               GTT^AAC
HphI         1758  GGTGA                GGTGA(8/7)
MamI          511  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2151  TGGCCA               TGG^CCA
MscI         2151  TGGCCA               TGG^CCA
MspA1I       1264  CAGCTG               C[AC]G^C[GT]G
MvnI           30  CGCG                 CG^CG
PleI         1785  GAGTC                GAGTC(4/5)
PshBI         906  ATTAAT               AT^TAAT
PvuII        1264  CAGCTG               CAG^CTG
SacI          571  GAGCTC               GAGCT^C
SapI         1635  GAAGAGC              GCTCTTC(1/4)
SgrAI          66  CACCGGCG             C[GA]^CCGG[CT]G
SmaI           12  CCCGGG               CCC^GGG
SphI         1611  GCATGC               GCATG^C
SspI          250  AATATT               AAT^ATT
Tsp45I       1742  GTGAC                ^GT[GC]AC
XmaCI          12  CCCGGG               C^CCGGG
XmaI           12  CCCGGG               C^CCGGG
XmnI          994  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Alw44I       1291  GTGCAC               G^TGCAC
             1771  GTGCAC              
AlwNI         556  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              596  CAGCCACTG           
ApaLI        1291  GTGCAC               G^TGCAC
             1771  GTGCAC              
BanI           64  GGCACC               G^G[CT][GA]CC
              173  GGTGCC              
BbvCI         850  CCTCAGC              CCTCAGC(-5/-2)
             1564  GCTGAGG             
BclI          516  TGATCA               T^GATCA
             2070  TGATCA              
BglII         303  AGATCT               A^GATCT
              429  AGATCT              
BpmI          296  CTGGAG               CTGGAG(16/14)
             1588  CTGGAG              
BsgI         1780  GTGCAG               GTGCAG(16/14)
             2018  GTGCAG              
BspHI         261  TCATGA               T^CATGA
             2172  TCATGA              
BsrBI           4  GAGCGG               CCGCTC(-3/-3)
               55  GAGCGG              
BsrDI         280  GCAATG               GCAATG(2/0)
             1351  GCAATG              
BstAPI        666  GCATCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1773  GCACACTGTGC         
DraIII       1294  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1774  CACACTGTG           
DsaI          626  CCATGG               C^C[GA][CT]GG
              812  CCATGG              
EaeI           22  CGGCCG               [CT]^GGCC[GA]
             2151  TGGCCA              
FbaI          516  TGATCA               T^GATCA
             2070  TGATCA              
HincII        871  GTTGAC               GT[CT]^[GA]AC
             2226  GTTAAC              
HindII        871  GTTGAC               GT[CT]^[GA]AC
             2226  GTTAAC              
NcoI          626  CCATGG               C^CATGG
              812  CCATGG              
NspI          730  GCATGT               [GA]CATG^[CT]
             1611  GCATGC              
PpuMI         845  GGGACCC              [GA]G^G[AT]CC[CT]
              864  AGGACCT             
PstI          553  CTGCAG               CTGCA^G
              673  CTGCAG              
RcaI          261  TCATGA               T^CATGA
             2172  TCATGA              
StuI          224  AGGCCT               AGG^CCT
              655  AGGCCT              
TaqI          396  TCGA                 T^CGA
              426  TCGA                
TthHB8I       396  TCGA                 T^CGA
              426  TCGA                
XbaI         1116  TCTAGA               T^CTAGA
             2010  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BamHI       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpuAI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       StyI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       XhoII       
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