Restriction map for
pF1KE0202
(Circular) (Six-base) MAPSORT of: pF1KE0202.seq Check: 3986 from: 1 to: 3917
pF1KE0202 3917 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 955 955
Size: 3917
Acc65I G'GTAC_C
Cuts at: 933 933
Size: 3917
AclI AA'CG_TT
Cuts at: 3757 3757
Size: 3917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 905 1742 2174 2921 3189 602
Size: 303 837 432 747 268 1330
Fragments arranged by size:
1330 837 747 432 303 268
AflIII A'CryG_T
Cuts at: 1269 2373 3350 1269
Size: 1104 977 1836
Fragments arranged by size:
1836 1104 977
AgeI A'CCGG_T
Cuts at: 2305 2305
Size: 3917
AlwNI CAG_nnn'CTG
Cuts at: 1222 2789 1222
Size: 1567 2350
ApaLI G'TGCA_C
Cuts at: 2687 2687
Size: 3917
ApoI r'AATT_y
Cuts at: 241 447 782 921 3270 3526 241
Size: 206 335 139 2349 256 632
Fragments arranged by size:
2349 632 335 256 206 139
AseI AT'TA_AT
Cuts at: 20 20
Size: 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
AvaI C'yCGr_G
Cuts at: 937 937
Size: 3917
BamHI G'GATC_C
Cuts at: 942 942
Size: 3917
BanI G'GyrC_C
Cuts at: 933 1347 1595 1630 933
Size: 414 248 35 3220
Fragments arranged by size:
3220 414 248 35
BanII G_rGCy'C
Cuts at: 931 1961 931
Size: 1030 2887
BbeI G_GCGC'C
Cuts at: 1351 1599 1351
Size: 248 3669
BbsI GAAGACnn'nnnn_
Cuts at: 847 893 847
Size: 46 3871
BciVI GTATCCnnnnn_n'
Cuts at: 1815 2576 3917 1815
Size: 761 1341 1815
Fragments arranged by size:
1815 1341 761
BclI T'GATC_A
Cuts at: 552 1437 552
Size: 885 3032
BglII A'GATC_T
Cuts at: 411 1432 3469 411
Size: 1021 2037 859
Fragments arranged by size:
2037 1021 859
BlpI GC'TnA_GC
Cuts at: 1029 1029
Size: 3917
Bme1580I G_kGCm'C
Cuts at: 1542 1635 2691 1542
Size: 93 1056 2768
Fragments arranged by size:
2768 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1129 1210 1545 3689 1129
Size: 81 335 2144 1357
Fragments arranged by size:
2144 1357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 3917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 359 154
Size: 205 3712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1972 2464 2762 1093
Size: 879 492 298 2248
Fragments arranged by size:
2248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3621 3621
Size: 3917
BsaAI yAC'GTr
Cuts at: 1270 1270
Size: 3917
BsaBI GATnn'nnATC
Cuts at: 6 1431 3468 6
Size: 1425 2037 455
Fragments arranged by size:
2037 1425 455
BsaHI Gr'CG_yC
Cuts at: 1348 1596 2298 3794 1348
Size: 248 702 1496 1471
Fragments arranged by size:
1496 1471 702 248
BsaWI w'CCGG_w
Cuts at: 648 1106 1627 2305 2579 2726 3137 648
Size: 458 521 678 274 147 411 1428
Fragments arranged by size:
1428 678 521 458 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 601 682 685 700 172
Size: 429 81 3 15 3389
Fragments arranged by size:
3389 429 81 15 3
BseYI C'CCAG_C
Cuts at: 289 1342 2100 2677 3173 289
Size: 1053 758 577 496 1033
Fragments arranged by size:
1053 1033 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1459 1505 2713 104
Size: 1355 46 1208 1308
Fragments arranged by size:
1355 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 931 1709 1899 2691 931
Size: 778 190 792 2157
Fragments arranged by size:
2157 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 931 1542 1635 1709 1899 1961 2691 931
Size: 611 93 74 190 62 730 2157
Fragments arranged by size:
2157 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3137 3137
Size: 3917
BspHI T'CATG_A
Cuts at: 3093 3093
Size: 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 712 967 1181 1483 1864 712
Size: 255 214 302 381 2765
Fragments arranged by size:
2765 381 302 255 214
BsrBI CCG'CTC
Cuts at: 50 2209 2263 3747 3912 50
Size: 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1829 1829
Size: 3917
BsrFI r'CCGG_y
Cuts at: 1915 2096 2305 1915
Size: 181 209 3527
Fragments arranged by size:
3527 209 181
BssSI C'ACGA_G
Cuts at: 2188 2546 2188
Size: 358 3559
BstAPI GCAn_nnn'nTGC
Cuts at: 1294 1294
Size: 3917
BstBI TT'CG_AA
Cuts at: 306 2278 306
Size: 1972 1945
BstYI r'GATC_y
Cuts at: 1 148 411 942 1409 1432 1767 2013 3025
Size: 147 263 531 467 23 335 246 1012
Cuts at: 3025 3111 3123 3469 1
Size: 86 12 346 449
Fragments arranged by size:
1012 531 467 449 346 335 263 246
147 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3917
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 620 1867 2048 2211 620
Size: 1247 181 163 2326
Fragments arranged by size:
2326 1247 181 163
BtsI GCAGTG_nn'
Cuts at: 1160 1160
Size: 3917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 375 410 375
Size: 35 3882
DraI TTT'AAA
Cuts at: 916 916
Size: 3917
DrdI GACnn_nn'nnGTC
Cuts at: 1623 2481 1623
Size: 858 3059
EaeI y'GGCC_r
Cuts at: 1502 1676 2067 2094 3439 1502
Size: 174 391 27 1345 1980
Fragments arranged by size:
1980 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1502 1502
Size: 3917
EarI CTCTTCn'nnn_
Cuts at: 578 614 620 659 671 677 740 824 833
Size: 36 6 39 12 6 63 84 9
Cuts at: 833 862 1940 2150 578
Size: 29 1078 210 2345
Fragments arranged by size:
2345 1078 210 84 63 39 36 29
12 9 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2125 2435 2581 3727 2125
Size: 310 146 1146 2315
Fragments arranged by size:
2315 1146 310 146
EcoICRI GAG'CTC
Cuts at: 929 929
Size: 3917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 359 602 905 1742 2174 2921 3189 154
Size: 205 243 303 837 432 747 268 882
Fragments arranged by size:
882 837 747 432 303 268 243 205
EcoO109I rG'GnC_Cy
Cuts at: 1056 1056
Size: 3917
EcoRI G'AATT_C
Cuts at: 921 921
Size: 3917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 871 1936 871
Size: 1065 2852
FspI TGC'GCA
Cuts at: 1698 1698
Size: 3917
HaeII r_GCGC'y
Cuts at: 574 1351 1599 2621 3600 574
Size: 777 248 1022 979 891
Fragments arranged by size:
1022 979 891 777 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1759 1789 1791 1821 1759
Size: 30 2 30 3855
Fragments arranged by size:
3855 30 30 2
HincII GTy'rAC
Cuts at: 956 956
Size: 3917
KasI G'GCGC_C
Cuts at: 1347 1595 1347
Size: 248 3669
KpnI G_GTAC'C
Cuts at: 937 937
Size: 3917
MluI A'CGCG_T
Cuts at: 3350 3350
Size: 3917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2588 2772 2588
Size: 184 3733
MscI TGG'CCA
Cuts at: 1678 1678
Size: 3917
MslI CAynn'nnrTG
Cuts at: 112 1906 2033 112
Size: 1794 127 1996
Fragments arranged by size:
1996 1794 127
MspA1I CmG'CkG
Cuts at: 1028 1342 1702 2715 2960 1028
Size: 314 360 1013 245 1985
Fragments arranged by size:
1985 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 3917
NarI GG'CG_CC
Cuts at: 1348 1596 1348
Size: 248 3669
NcoI C'CATG_G
Cuts at: 107 107
Size: 3917
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 3917
NspI r_CATG'y
Cuts at: 970 1470 2377 970
Size: 500 907 2510
Fragments arranged by size:
2510 907 500
PciI A'CATG_T
Cuts at: 2373 2373
Size: 3917
PmeI GTTT'AAAC
Cuts at: 916 916
Size: 3917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3885
PstI C_TGCA'G
Cuts at: 527 964 1649 527
Size: 437 685 2795
Fragments arranged by size:
2795 685 437
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3917
PvuII CAG'CTG
Cuts at: 1342 1702 1342
Size: 360 3557
RsrII CG'GwC_CG
Cuts at: 2112 2112
Size: 3917
SacI G_AGCT'C
Cuts at: 931 931
Size: 3917
SalI G'TCGA_C
Cuts at: 954 954
Size: 3917
SapI GCTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
SbfI CC_TGCA'GG
Cuts at: 964 964
Size: 3917
ScaI AGT'ACT
Cuts at: 3434 3434
Size: 3917
SfcI C'TryA_G
Cuts at: 33 523 960 1645 2638 2829 33
Size: 490 437 685 993 191 1121
Fragments arranged by size:
1121 993 685 490 437 191
SfoI GGC'GCC
Cuts at: 1349 1597 1349
Size: 248 3669
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
SmaI CCC'GGG
Cuts at: 939 939
Size: 3917
SmlI C'TyrA_G
Cuts at: 1072 1987 2479 2741 1072
Size: 915 492 262 2248
Fragments arranged by size:
2248 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3917
SphI G_CATG'C
Cuts at: 970 1470 970
Size: 500 3417
StuI AGG'CCT
Cuts at: 215 215
Size: 3917
StyI C'CwwG_G
Cuts at: 107 1051 1405 107
Size: 944 354 2619
Fragments arranged by size:
2619 944 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1500 1633 2092 2301 1500
Size: 133 459 209 3116
Fragments arranged by size:
3116 459 209 133
TatI w'GTAC_w
Cuts at: 418 3432 418
Size: 3014 903
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 3885
Tth111I GACn'n_nGTC
Cuts at: 1714 1714
Size: 3917
XbaI T'CTAG_A
Cuts at: 948 948
Size: 3917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1412 1412
Size: 3917
XmaI C'CCGG_G
Cuts at: 937 937
Size: 3917
XmnI GAAnn'nnTTC
Cuts at: 782 782
Size: 3917
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI