Restriction map for pF1KE0171
 (Circular) (Six-base) MAPSORT of: pF1KE0171.seq  Check: 7355  from: 1  to: 3779



pF1KE0171  3779 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:    817     817 
   Size:       3779
 
Acc65I G'GTAC_C

Cuts at:    795     795 
   Size:       3779
 
AclI AA'CG_TT

Cuts at:   3619    3619 
   Size:       3779
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    343     391     664    1604    2036    2783    3051     343 
   Size:         48     273     940     432     747     268    1071

  Fragments arranged by size: 

                1071     940     747     432     273     268      48
 
AflIII A'CryG_T

Cuts at:   1131    2235    3212    1131 
   Size:       1104     977    1698

  Fragments arranged by size: 

                1698    1104     977
 
AgeI A'CCGG_T

Cuts at:   2167    2167 
   Size:       3779
 
AlwNI CAG_nnn'CTG

Cuts at:   1084    2651    1084 
   Size:       1567    2212
 
ApaLI G'TGCA_C

Cuts at:   2549    2549 
   Size:       3779
 
ApoI r'AATT_y

Cuts at:    783    3132    3388     783 
   Size:       2349     256    1174

  Fragments arranged by size: 

                2349    1174     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3779
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3779
 
AvaI C'yCGr_G

Cuts at:    799     799 
   Size:       3779
 
BamHI G'GATC_C

Cuts at:    609     804     609 
   Size:        195    3584
 
BanI G'GyrC_C

Cuts at:    130     214     795    1209    1457    1492     130 
   Size:         84     581     414     248      35    2417

  Fragments arranged by size: 

                2417     581     414     248      84      35
 
BanII G_rGCy'C

Cuts at:    172     793    1823     172 
   Size:        621    1030    2128

  Fragments arranged by size: 

                2128    1030     621
 
BbeI G_GCGC'C

Cuts at:    218    1213    1461     218 
   Size:        995     248    2536

  Fragments arranged by size: 

                2536     995     248
 
BbvCI CC'TCA_GC

Cuts at:    421     421 
   Size:       3779
 
BciVI GTATCCnnnnn_n'

Cuts at:   1677    2438    3779    1677 
   Size:        761    1341    1677

  Fragments arranged by size: 

                1677    1341     761
 
BclI T'GATC_A

Cuts at:   1299    1299 
   Size:       3779
 
BglII A'GATC_T

Cuts at:   1294    3331    1294 
   Size:       2037    1742
 
BlpI GC'TnA_GC

Cuts at:    891     891 
   Size:       3779
 
Bme1580I G_kGCm'C

Cuts at:   1404    1497    2553    1404 
   Size:         93    1056    2630

  Fragments arranged by size: 

                2630    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    744     991    1072    1407    3551     744 
   Size:        247      81     335    2144     972

  Fragments arranged by size: 

                2144     972     335     247      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2146    2146 
   Size:       3779
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    156     156 
   Size:       3779
 
Bpu10I CC'TnA_GC

Cuts at:    421     540     421 
   Size:        119    3660
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    955    1834    2326    2624     955 
   Size:        879     492     298    2110

  Fragments arranged by size: 

                2110     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3483    3483 
   Size:       3779
 
BsaAI yAC'GTr

Cuts at:    282    1132     282 
   Size:        850    2929
 
BsaBI GATnn'nnATC

Cuts at:      6    1293    3330       6 
   Size:       1287    2037     455

  Fragments arranged by size: 

                2037    1287     455
 
BsaHI Gr'CG_yC

Cuts at:    215    1210    1458    2160    3656     215 
   Size:        995     248     702    1496     338

  Fragments arranged by size: 

                1496     995     702     338     248
 
BsaWI w'CCGG_w

Cuts at:    150     968    1489    2167    2441    2588    2999     150 
   Size:        818     521     678     274     147     411     930

  Fragments arranged by size: 

                 930     818     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    319     319 
   Size:       3779
 
BseYI C'CCAG_C

Cuts at:    645    1204    1962    2539    3035     645 
   Size:        559     758     577     496    1389

  Fragments arranged by size: 

                1389     758     577     559     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    238     350     238 
   Size:        112    3667
 
BsiEI CG_ry'CG

Cuts at:    104    1321    1367    2575     104 
   Size:       1217      46    1208    1308

  Fragments arranged by size: 

                1308    1217    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    668     793    1571    1761    2553     668 
   Size:        125     778     190     792    1894

  Fragments arranged by size: 

                1894     792     778     190     125
 
Bsp1286I G_dGCh'C

Cuts at:    172     668     793    1404    1497    1571    1761    1823    2553 
   Size:        496     125     611      93      74     190      62     730

Cuts at:   2553     172 
   Size:       1398

  Fragments arranged by size: 

                1398     730     611     496     190     125      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2999    2999 
   Size:       3779
 
BspHI T'CATG_A

Cuts at:   2955    2955 
   Size:       3779
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    206     829    1043    1345    1726     206 
   Size:        623     214     302     381    2259

  Fragments arranged by size: 

                2259     623     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2071    2125    3609    3774      50 
   Size:       2021      54    1484     165      55

  Fragments arranged by size: 

                2021    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1691    1691 
   Size:       3779
 
BsrFI r'CCGG_y

Cuts at:   1777    1958    2167    1777 
   Size:        181     209    3389

  Fragments arranged by size: 

                3389     209     181
 
BssSI C'ACGA_G

Cuts at:   2050    2408    2050 
   Size:        358    3421
 
BstAPI GCAn_nnn'nTGC

Cuts at:    246    1156     246 
   Size:        910    2869
 
BstBI TT'CG_AA

Cuts at:   2140    2140 
   Size:       3779
 
BstYI r'GATC_y

Cuts at:      1     609     804    1271    1294    1629    1875    2887    2973 
   Size:        608     195     467      23     335     246    1012      86

Cuts at:   2973    2985    3331       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     608     467     449     346     335     246     195
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1729    1910    2073    1729 
   Size:        181     163    3435

  Fragments arranged by size: 

                3435     181     163
 
BtsI GCAGTG_nn'

Cuts at:    128     613     640    1022     128 
   Size:        485      27     382    2885

  Fragments arranged by size: 

                2885     485     382      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    366     401     366 
   Size:         35    3744
 
DraI TTT'AAA

Cuts at:    778     778 
   Size:       3779
 
DrdI GACnn_nn'nnGTC

Cuts at:   1485    2343    1485 
   Size:        858    2921
 
EaeI y'GGCC_r

Cuts at:    326    1364    1538    1929    1956    3301     326 
   Size:       1038     174     391      27    1345     804

  Fragments arranged by size: 

                1345    1038     804     391     174      27
 
EagI C'GGCC_G

Cuts at:   1364    1364 
   Size:       3779
 
EarI CTCTTCn'nnn_

Cuts at:    362    1802    2012     362 
   Size:       1440     210    2129

  Fragments arranged by size: 

                2129    1440     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1987    2297    2443    3589    1987 
   Size:        310     146    1146    2177

  Fragments arranged by size: 

                2177    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    791     791 
   Size:       3779
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    156     343     391     664    1604    2036    2783    3051     156 
   Size:        187      48     273     940     432     747     268     884

  Fragments arranged by size: 

                 940     884     747     432     273     268     187      48
            
 
EcoO109I rG'GnC_Cy

Cuts at:    918     918 
   Size:       3779
 
EcoRI G'AATT_C

Cuts at:    783     783 
   Size:       3779
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1798    1798 
   Size:       3779
 
FspI TGC'GCA

Cuts at:   1560    1560 
   Size:       3779
 
HaeII r_GCGC'y

Cuts at:    218    1213    1461    2483    3462     218 
   Size:        995     248    1022     979     535

  Fragments arranged by size: 

                1022     995     979     535     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1621    1651    1653    1683    1621 
   Size:         30       2      30    3717

  Fragments arranged by size: 

                3717      30      30       2
 
HincII GTy'rAC

Cuts at:    818     818 
   Size:       3779
 
KasI G'GCGC_C

Cuts at:    214    1209    1457     214 
   Size:        995     248    2536

  Fragments arranged by size: 

                2536     995     248
 
KpnI G_GTAC'C

Cuts at:    799     799 
   Size:       3779
 
MluI A'CGCG_T

Cuts at:   3212    3212 
   Size:       3779
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2450    2634    2450 
   Size:        184    3595
 
MscI TGG'CCA

Cuts at:    328    1540     328 
   Size:       1212    2567
 
MslI CAynn'nnrTG

Cuts at:   1768    1895    1768 
   Size:        127    3652
 
MspA1I CmG'CkG

Cuts at:    144     252     890    1204    1564    2577    2822     144 
   Size:        108     638     314     360    1013     245    1101

  Fragments arranged by size: 

                1101    1013     638     360     314     245     108
 
NaeI GCC'GGC

Cuts at:   1960    1960 
   Size:       3779
 
NarI GG'CG_CC

Cuts at:    215    1210    1458     215 
   Size:        995     248    2536

  Fragments arranged by size: 

                2536     995     248
 
NgoMIV G'CCGG_C

Cuts at:   1958    1958 
   Size:       3779
 
NspI r_CATG'y

Cuts at:    832    1332    2239     832 
   Size:        500     907    2372

  Fragments arranged by size: 

                2372     907     500
 
PciI A'CATG_T

Cuts at:   2235    2235 
   Size:       3779
 
PmeI GTTT'AAAC

Cuts at:    778     778 
   Size:       3779
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2918    2950    2918 
   Size:         32    3747
 
PstI C_TGCA'G

Cuts at:    826    1511     826 
   Size:        685    3094
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3779
 
PvuII CAG'CTG

Cuts at:    144     252    1204    1564     144 
   Size:        108     952     360    2359

  Fragments arranged by size: 

                2359     952     360     108
 
RsrII CG'GwC_CG

Cuts at:   1974    1974 
   Size:       3779
 
SacI G_AGCT'C

Cuts at:    793     793 
   Size:       3779
 
SalI G'TCGA_C

Cuts at:    816     816 
   Size:       3779
 
SapI GCTCTTCn'nnn_

Cuts at:    362    1802    2012     362 
   Size:       1440     210    2129

  Fragments arranged by size: 

                2129    1440     210
 
SbfI CC_TGCA'GG

Cuts at:    826     826 
   Size:       3779
 
ScaI AGT'ACT

Cuts at:   3296    3296 
   Size:       3779
 
SfcI C'TryA_G

Cuts at:     33     822    1507    2500    2691      33 
   Size:        789     685     993     191    1121

  Fragments arranged by size: 

                1121     993     789     685     191
 
SfoI GGC'GCC

Cuts at:    216    1211    1459     216 
   Size:        995     248    2536

  Fragments arranged by size: 

                2536     995     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3779
 
SmaI CCC'GGG

Cuts at:    801     801 
   Size:       3779
 
SmlI C'TyrA_G

Cuts at:    934    1849    2341    2603     934 
   Size:        915     492     262    2110

  Fragments arranged by size: 

                2110     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3779
 
SphI G_CATG'C

Cuts at:    832    1332     832 
   Size:        500    3279
 
StuI AGG'CCT

Cuts at:    632     699     632 
   Size:         67    3712
 
StyI C'CwwG_G

Cuts at:    329     534     913    1267     329 
   Size:        205     379     354    2841

  Fragments arranged by size: 

                2841     379     354     205
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1362    1495    1954    2163    1362 
   Size:        133     459     209    2978

  Fragments arranged by size: 

                2978     459     209     133
 
TatI w'GTAC_w

Cuts at:   3294    3294 
   Size:       3779
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3296    3328    3296 
   Size:         32    3747
 
Tth111I GACn'n_nGTC

Cuts at:   1576    1576 
   Size:       3779
 
XbaI T'CTAG_A

Cuts at:    810     810 
   Size:       3779
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    141    1274     141 
   Size:       1133    2646
 
XmaI C'CCGG_G

Cuts at:    799     799 
   Size:       3779
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     XmnI     ZraI
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