Restriction map for pF1KE0133
 (Linear) (Six-base) MAPSORT of: KIEE0133.seq  Check: 7394  from: 1  to: 783



KIEE0133.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     783 
   Size:        274     509
 
AflIII A'CryG_T

Cuts at:      0     260     778     783 
   Size:        260     518       5

  Fragments arranged by size: 

                 518     260       5
 
AlwNI CAG_nnn'CTG

Cuts at:      0     303     534     783 
   Size:        303     231     249

  Fragments arranged by size: 

                 303     249     231
 
ApaLI G'TGCA_C

Cuts at:      0      28     783 
   Size:         28     755
 
BanI G'GyrC_C

Cuts at:      0     126     323     386     783 
   Size:        126     197      63     397

  Fragments arranged by size: 

                 397     197     126      63
 
BbeI G_GCGC'C

Cuts at:      0     130     327     783 
   Size:        130     197     456

  Fragments arranged by size: 

                 456     197     130
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     781     783 
   Size:        781       2
 
BglI GCCn_nnn'nGGC

Cuts at:      0     154     671     783 
   Size:        154     517     112

  Fragments arranged by size: 

                 517     154     112
 
Bme1580I G_kGCm'C

Cuts at:      0      32     783 
   Size:         32     751
 
BmgBI CAC'GTC

Cuts at:      0     261     699     783 
   Size:        261     438      84

  Fragments arranged by size: 

                 438     261      84
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     508     783 
   Size:        508     275
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     459     783 
   Size:        459     324
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688     783 
   Size:        688      95
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     604     783 
   Size:        454     150     179

  Fragments arranged by size: 

                 454     179     150
 
BsaAI yAC'GTr

Cuts at:      0      33     510     783 
   Size:         33     477     273

  Fragments arranged by size: 

                 477     273      33
 
BsaHI Gr'CG_yC

Cuts at:      0     127     324     651     783 
   Size:        127     197     327     132

  Fragments arranged by size: 

                 327     197     132     127
 
BseYI C'CCAG_C

Cuts at:      0     198     237     783 
   Size:        198      39     546

  Fragments arranged by size: 

                 546     198      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     316     783 
   Size:        199     117     467

  Fragments arranged by size: 

                 467     199     117
 
BsiHKAI G_wGCw'C

Cuts at:      0      32     116     507     596     704     783 
   Size:         32      84     391      89     108      79

  Fragments arranged by size: 

                 391     108      89      84      79      32
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     447     658     783 
   Size:        447     211     125

  Fragments arranged by size: 

                 447     211     125
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     116     507     596     704     783 
   Size:         32      84     391      89     108      79

  Fragments arranged by size: 

                 391     108      89      84      79      32
 
BsrFI r'CCGG_y

Cuts at:      0      43     136     783 
   Size:         43      93     647

  Fragments arranged by size: 

                 647      93      43
 
BssSI C'ACGA_G

Cuts at:      0     175     783 
   Size:        175     608
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     303     502     534     783 
   Size:        303     199      32     249

  Fragments arranged by size: 

                 303     249     199      32
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     766     783 
   Size:        766      17
 
BtgI C'CryG_G

Cuts at:      0      38      59     131     272     332     377     402     783 
   Size:         38      21      72     141      60      45      25     381

  Fragments arranged by size: 

                 381     141      72      60      45      38      25      21
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     244     783 
   Size:        244     539
 
BtsI GCAGTG_nn'

Cuts at:      0     304     325     355     783 
   Size:        304      21      30     428

  Fragments arranged by size: 

                 428     304      30      21
 
DraIII CAC_nnn'GTG

Cuts at:      0     502     513     783 
   Size:        502      11     270

  Fragments arranged by size: 

                 502     270      11
 
EaeI y'GGCC_r

Cuts at:      0      35      41     134     155     449     515     783 
   Size:         35       6      93      21     294      66     268

  Fragments arranged by size: 

                 294     268      93      66      35      21       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     661     783 
   Size:        661     122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     688     783 
   Size:        274     414      95

  Fragments arranged by size: 

                 414     274      95
 
EcoO109I rG'GnC_Cy

Cuts at:      0     241     783 
   Size:        241     542
 
FseI GG_CCGG'CC

Cuts at:      0     140     783 
   Size:        140     643
 
FspI TGC'GCA

Cuts at:      0     538     783 
   Size:        538     245
 
HaeII r_GCGC'y

Cuts at:      0     130     327     535     783 
   Size:        130     197     208     248

  Fragments arranged by size: 

                 248     208     197     130
 
KasI G'GCGC_C

Cuts at:      0     126     323     783 
   Size:        126     197     460

  Fragments arranged by size: 

                 460     197     126
 
MluI A'CGCG_T

Cuts at:      0     778     783 
   Size:        778       5
 
MscI TGG'CCA

Cuts at:      0     157     451     783 
   Size:        157     294     332

  Fragments arranged by size: 

                 332     294     157
 
MspA1I CmG'CkG

Cuts at:      0     334     404     520     783 
   Size:        334      70     116     263

  Fragments arranged by size: 

                 334     263     116      70
 
NaeI GCC'GGC

Cuts at:      0     138     783 
   Size:        138     645
 
NarI GG'CG_CC

Cuts at:      0     127     324     783 
   Size:        127     197     459

  Fragments arranged by size: 

                 459     197     127
 
NcoI C'CATG_G

Cuts at:      0     272     783 
   Size:        272     511
 
NgoMIV G'CCGG_C

Cuts at:      0     136     783 
   Size:        136     647
 
PasI CC'CwG_GG

Cuts at:      0     480     783 
   Size:        480     303
 
PmlI CAC'GTG

Cuts at:      0      33     510     783 
   Size:         33     477     273

  Fragments arranged by size: 

                 477     273      33
 
PpuMI rG'GwC_Cy

Cuts at:      0     241     783 
   Size:        241     542
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     172     204     783 
   Size:        172      32     579

  Fragments arranged by size: 

                 579     172      32
 
SacII CC_GC'GG

Cuts at:      0     335     405     783 
   Size:        335      70     378

  Fragments arranged by size: 

                 378     335      70
 
SanDI GG'GwC_CC

Cuts at:      0     241     783 
   Size:        241     542
 
SfoI GGC'GCC

Cuts at:      0     128     325     783 
   Size:        128     197     458

  Fragments arranged by size: 

                 458     197     128
 
SmlI C'TyrA_G

Cuts at:      0     433     619     783 
   Size:        433     186     164

  Fragments arranged by size: 

                 433     186     164
 
StuI AGG'CCT

Cuts at:      0      22     783 
   Size:         22     761
 
StyI C'CwwG_G

Cuts at:      0     272     686     783 
   Size:        272     414      97

  Fragments arranged by size: 

                 414     272      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     490     556     764     783 
   Size:        490      66     208      19

  Fragments arranged by size: 

                 490     208      66      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     667     699     783 
   Size:        667      32      84

  Fragments arranged by size: 

                 667      84      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     550     783 
   Size:        550     233
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI    ApaLI     BanI     BbeI     BbsI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI    BsaAI    BsaHI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrFI    BssSI   BstAPI
    BstXI     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I     FseI     FspI    HaeII     KasI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     PasI     PmlI    PpuMI     PsrI
    SacII    SanDI     SfoI     SmlI     StuI     StyI    TaqII     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI   Bpu10I     BsaI
    BsaBI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MmeI     MslI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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