Restriction map for pF1KB7670
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        MaeI        MamI        MfeI        MflI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TfiI        Tth111I     
XbaI        XcmI        XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI          463  GAATTC               [GA]^AATT[CT]
AlwI          291  GGATC                GGATC(4/5)
ApaI          112  GGGCCC               GGGCC^C
ApoI          463  GAATTC               [GA]^AATT[CT]
BalI          959  TGGCCA               TGG^CCA
BanI          839  GGCACC               G^G[CT][GA]CC
BbvCI        1080  CCTCAGC              CCTCAGC(-5/-2)
BlpI          525  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      525  GCTCAGC              GC^T[ACGT]AGC
BsaI          717  GGTCTC               GGTCTC(1/5)
BsgI          342  GTGCAG               GTGCAG(16/14)
BsmBI         763  GAGACG               CGTCTC(1/5)
BspMI         521  ACCTGC               ACCTGC(4/8)
BsrBI         439  GAGCGG               CCGCTC(-3/-3)
BsrI          753  ACTGG                ACTGG(1/-1)
BssSI         229  CACGAG               CACGAG(-5/-1)
CelII         525  GCTCAGC              GC^T[ACGT]AGC
EcoRI         463  GAATTC               G^AATTC
HaeII         390  GGCGCT               [GA]GCGC^[CT]
HincII        211  GTCAAC               GT[CT]^[GA]AC
HindII        211  GTCAAC               GT[CT]^[GA]AC
KspI          646  CCGCGG               CCGC^GG
MaeII        1109  ACGT                 A^CGT
MaeIII        500  GTGAC                ^GT[ACGT]AC
MluNI         959  TGGCCA               TGG^CCA
MscI          959  TGGCCA               TGG^CCA
MseI         1072  TTAA                 T^TAA
MslI           81  CATCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          591  GCCGGC               GCC^GGC
NcoI          962  CCATGG               C^CATGG
NgoMIV        591  GCCGGC               G^CCGGC
PflMI         870  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         1120  CTGCAG               CTGCA^G
SacII         646  CCGCGG               CCGC^GG
SbfI         1119  CCTGCAGG             CCTGCA^GG
SfcI         1120  CTGCAG               C^T[GA][CT]AG
SmaI          109  CCCGGG               CCC^GGG
SmlI          472  CTCAAG               C^T[CT][GA]AG
Sse8387I     1119  CCTGCAGG             CCTGCA^GG
StuI          704  AGGCCT               AGG^CCT
Tru9I        1072  TTAA                 T^TAA
Tsp45I        500  GTGAC                ^GT[GC]AC
Tsp509I       464  AATT                 ^AATT
Van91I        870  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         109  CCCGGG               C^CCGGG
XmaI          109  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          303  GTAC                 GT^AC
              405  GTAC                
AspHI          94  GAGCAC               G[AT]GC[AT]^C
              469  GTGCTC              
AvaI           19  CCCGAG               C^[CT]CG[GA]G
              109  CCCGGG              
BglI          697  GCCTTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              974  GCCCCTACGGC         
BpmI           28  CTGGAG               CTGGAG(16/14)
              394  CTGGAG              
BsiEI         192  CGACCG               CG[GA][CT]^CG
             1146  CGACCG              
BsiHKAI        94  GAGCAC               G[AT]GC[AT]^C
              469  GTGCTC              
BsmFI         581  GGGAC                GGGAC(10/14)
               17  GTCCC               
BsoBI          19  CCCGAG               C^[CT]CG[GA]G
              109  CCCGGG              
BsrFI         591  GCCGGC               [GA]^CCGG[CT]
             1148  ACCGGC              
BstAPI         96  GCACGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              344  GCAGCGGCTGC         
BstXI          65  CCAAGATGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              896  CCAGCCGCATGG        
Cfr10I        591  GCCGGC               [GA]^CCGG[CT]
             1148  ACCGGC              
DsaI          646  CCGCGG               C^C[GA][CT]GG
              962  CCATGG              
EcoT14I       596  CCAAGG               C^C[AT][AT]GG
              962  CCATGG              
RsaI          303  GTAC                 GT^AC
              405  GTAC                
StyI          596  CCAAGG               C^C[AT][AT]GG
              962  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        TspRI       TthHB8I     
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