Restriction map for pF1KB5616
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BbeI        BcgI        BclI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrGI       
BssHII      BssSI       Bst1107I    BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
Eam1105I    Eco47III    Eco81I      EcoNI       EcoRV       
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        MamI        
MfeI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NruI        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        SfiI        SfoI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        481  GGTACC               G^GTACC
AccI         2331  GTCTAC               GT^[AC][GT]AC
AflII        2011  CTTAAG               C^TTAAG
Asp700I       369  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       481  GGTACC               G^GTACC
AspI         2062  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1480  GGATCC               G^GATCC
BanI          481  GGTACC               G^G[CT][GA]CC
BbrPI         652  CACGTG               CAC^GTG
BbsI          618  GAAGAC               GAAGAC(2/6)
BbvCI         282  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1841  GTATCC               GTATCC(6/5)
BfrI         2011  CTTAAG               C^TTAAG
BpuAI         618  GAAGAC               GAAGAC(2/6)
BsmI         1531  GAATGC               GAATGC(1/-1)
BspLU11I      569  ACATGT               A^CATGT
BsrBI          25  CCGCTC               CCGCTC(-3/-3)
BsrFI        1738  ACCGGC               [GA]^CCGG[CT]
BstAPI       1631  GCAAGTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         366  TTCGAA               TT^CGAA
BstXI         929  CCACTACCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1480  GGATCC               [GA]^GATC[CT]
Cfr10I       1738  ACCGGC               [GA]^CCGG[CT]
DrdI         2062  GACTGTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2254  GAATTC               G^AATTC
EcoT22I       800  ATGCAT               ATGCA^T
HgaI          660  GACGC                GACGC(5/10)
KpnI          481  GGTACC               GGTAC^C
MflI         1480  GGATCC               [GA]^GATC[CT]
MluI         1762  ACGCGT               A^CGCGT
NsiI          800  ATGCAT               ATGCA^T
NspV          366  TTCGAA               TT^CGAA
PflFI        2062  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         652  CACGTG               CAC^GTG
PmlI          652  CACGTG               CAC^GTG
PpuMI        1435  GGGACCT              [GA]G^G[AT]CC[CT]
PstI         1560  CTGCAG               CTGCA^G
SapI         2125  GAAGAGC              GCTCTTC(1/4)
SexAI         671  ACCAGGT              A^CC[AT]GGT
SfuI          366  TTCGAA               TT^CGAA
SmaI         1541  CCCGGG               CCC^GGG
Tth111I      2062  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1480  GGATCC               [GA]^GATC[CT]
XmaCI        1541  CCCGGG               C^CCGGG
XmaI         1541  CCCGGG               C^CCGGG
XmnI          369  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI         1481  GATCC                GGATC(4/5)
             1480  GGATC               
ApaI           79  GGGCCC               GGGCC^C
             2145  GGGCCC              
AvaI          260  CCCGAG               C^[CT]CG[GA]G
             1541  CCCGGG              
BalI          491  TGGCCA               TGG^CCA
             1695  TGGCCA              
BfaI          268  CTAG                 C^TAG
             2096  CTAG                
BsaAI         652  CACGTG               [CT]AC^GT[GA]
             2349  CACGTA              
BseRI        2227  CTCCTC               GAGGAG(10/8)
             1501  GAGGAG              
BsoBI         260  CCCGAG               C^[CT]CG[GA]G
             1541  CCCGGG              
BstEII        675  GGTGACC              G^GT[ACGT]ACC
             1362  GGTCACC             
BstPI         675  GGTGACC              G^GT[ACGT]ACC
             1362  GGTCACC             
EarI         1168  GAAGAG               CTCTTC(1/4)
             2125  GAAGAG              
EcoO65I       675  GGTGACC              G^GT[ACGT]ACC
             1362  GGTCACC             
HaeII          45  GGCGCT               [GA]GCGC^[CT]
               54  GGCGCT              
Ksp632I      1168  GAAGAG               CTCTTC(1/4)
             2125  GAAGAG              
KspI           13  CCGCGG               CCGC^GG
             1809  CCGCGG              
MaeI          268  CTAG                 C^TAG
             2096  CTAG                
MluNI         491  TGGCCA               TGG^CCA
             1695  TGGCCA              
MscI          491  TGGCCA               TGG^CCA
             1695  TGGCCA              
MseI         1907  TTAA                 T^TAA
             2012  TTAA                
NotI            9  GCGGCCGC             GC^GGCCGC
             1805  GCGGCCGC            
NspI          569  ACATGT               [GA]CATG^[CT]
             1778  GCATGT              
PvuII        1704  CAGCTG               CAG^CTG
             1830  CAGCTG              
SacI          108  GAGCTC               GAGCT^C
             1045  GAGCTC              
SacII          13  CCGCGG               CCGC^GG
             1809  CCGCGG              
Tru9I        1907  TTAA                 T^TAA
             2012  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwNI       ApoI        AspHI       AvaII       
BanII       BbvI        BcnI        BglI        BmyI        
BpmI        BsaJI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EclXI       Eco52I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        
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