Restriction map for pF1KB5616
 (Circular) (Six-base) MAPSORT of: pF1KB5616.seq  Check: 3714  from: 1  to: 5477



pF1KB5616  5477 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    492     742    1312    1891    2002     492 
   Size:        250     570     579     111    3967

  Fragments arranged by size: 

                3967     579     570     250     111
 
AccI GT'mk_AC

Cuts at:   2440    2515    2440 
   Size:         75    5402
 
Acc65I G'GTAC_C

Cuts at:    589    2493     589 
   Size:       1904    3573
 
AclI AA'CG_TT

Cuts at:   5317    5317 
   Size:       5477
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    325     340    1295    1359    3302    3734    4481    4749     325 
   Size:         15     955      64    1943     432     747     268    1053

  Fragments arranged by size: 

                1943    1053     955     747     432     268      64      15
            
 
AflII C'TTAA_G

Cuts at:   2119    2119 
   Size:       5477
 
AflIII A'CryG_T

Cuts at:    677     759    1870    2829    3933    4910     677 
   Size:         82    1111     959    1104     977    1244

  Fragments arranged by size: 

                1244    1111    1104     977     959      82
 
AgeI A'CCGG_T

Cuts at:   3865    3865 
   Size:       5477
 
AleI CACnn'nnGTG

Cuts at:   1715    1715 
   Size:       5477
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1918    1918 
   Size:       5477
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1224    1256    1224 
   Size:         32    5445
 
AlwNI CAG_nnn'CTG

Cuts at:    234     585    1117    2782    4349     234 
   Size:        351     532    1665    1567    1362

  Fragments arranged by size: 

                1665    1567    1362     532     351
 
ApaI G_GGCC'C

Cuts at:    191    2257     191 
   Size:       2066    3411
 
ApaLI G'TGCA_C

Cuts at:   4247    4247 
   Size:       5477
 
ApoI r'AATT_y

Cuts at:    480    2362    2371    2481    4830    5086     480 
   Size:       1882       9     110    2349     256     871

  Fragments arranged by size: 

                2349    1882     871     256     110       9
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5477
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5477
 
AvaI C'yCGr_G

Cuts at:    368    1649    2497     368 
   Size:       1281     848    3348

  Fragments arranged by size: 

                3348    1281     848
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    183     216     573     606     183 
   Size:         33     357      33    5054

  Fragments arranged by size: 

                5054     357      33      33
 
BamHI G'GATC_C

Cuts at:   1588    2502    1588 
   Size:        914    4563
 
BanI G'GyrC_C

Cuts at:    589    2493    2907    3155    3190     589 
   Size:       1904     414     248      35    2876

  Fragments arranged by size: 

                2876    1904     414     248      35
 
BanII G_rGCy'C

Cuts at:    191     220     247     289     406    1157    1963    2058    2257 
   Size:         29      27      42     117     751     806      95     199

Cuts at:   2257    2491    3521     191 
   Size:        234    1030    2147

  Fragments arranged by size: 

                2147    1030     806     751     234     199     117      95
                  42      29      27
 
BbeI G_GCGC'C

Cuts at:   2911    3159    2911 
   Size:        248    5229
 
BbsI GAAGACnn'nnnn_

Cuts at:    733     733 
   Size:       5477
 
BbvCI CC'TCA_GC

Cuts at:    391     391 
   Size:       5477
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    5443
 
BciVI GTATCCnnnnn_n'

Cuts at:   1960    3375    4136    5477    1960 
   Size:       1415     761    1341    1960

  Fragments arranged by size: 

                1960    1415    1341     761
 
BclI T'GATC_A

Cuts at:   2997    2997 
   Size:       5477
 
BfrBI ATG'CAT

Cuts at:    910     910 
   Size:       5477
 
BglI GCCn_nnn'nGGC

Cuts at:   1498    1581    1679    1498 
   Size:         83      98    5296

  Fragments arranged by size: 

                5296      98      83
 
BglII A'GATC_T

Cuts at:   2992    5029    2992 
   Size:       2037    3440
 
BlpI GC'TnA_GC

Cuts at:   2589    2589 
   Size:       5477
 
Bme1580I G_kGCm'C

Cuts at:    191     336    1990    2257    2470    3102    3195    4251     191 
   Size:        145    1654     267     213     632      93    1056    1417

  Fragments arranged by size: 

                1654    1417    1056     632     267     213     145      93
            
 
BmgBI CAC'GTC

Cuts at:   2438    2438 
   Size:       5477
 
BmrI ACTGGGnnnn_n'

Cuts at:    349    1587    2689    2770    3105    5249     349 
   Size:       1238    1102      81     335    2144     577

  Fragments arranged by size: 

                2144    1238    1102     577     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1809    3844    1809 
   Size:       2035    3442
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    428     918    1192     428 
   Size:        490     274    4713

  Fragments arranged by size: 

                4713     490     274
 
Bpu10I CC'TnA_GC

Cuts at:    391    1794     391 
   Size:       1403    4074
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    413    1318    1336    2653    3532    4024    4322     413 
   Size:        905      18    1317     879     492     298    1568

  Fragments arranged by size: 

                1568    1317     905     879     492     298      18
 
BsaI GGTCTCn'nnnn_

Cuts at:   5181    5181 
   Size:       5477
 
BsaAI yAC'GTr

Cuts at:    762    2459    2830     762 
   Size:       1697     371    3409

  Fragments arranged by size: 

                3409    1697     371
 
BsaBI GATnn'nnATC

Cuts at:      6    2991    5028       6 
   Size:       2985    2037     455

  Fragments arranged by size: 

                2985    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2908    3156    3858    5354    2908 
   Size:        248     702    1496    3031

  Fragments arranged by size: 

                3031    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2666    3187    3865    4139    4286    4697    2666 
   Size:        521     678     274     147     411    3446

  Fragments arranged by size: 

                3446     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    443     473    1018    1048    1352    1382    1747    1777     443 
   Size:         30     545      30     304      30     365      30    4143

  Fragments arranged by size: 

                4143     545     365     304      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1624    2326    1624 
   Size:        702    4775
 
BseYI C'CCAG_C

Cuts at:    156    1584    1628    2902    3660    4237    4733     156 
   Size:       1428      44    1274     758     577     496     900

  Fragments arranged by size: 

                1428    1274     900     758     577     496      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1830    1929    1992    1830 
   Size:         99      63    5315

  Fragments arranged by size: 

                5315      99      63
 
BsiEI CG_ry'CG

Cuts at:    104     121     133    1917    2355    3019    3065    4273     104 
   Size:         17      12    1784     438     664      46    1208    1308

  Fragments arranged by size: 

                1784    1308    1208     664     438      46      17      12
            
 
BsiHKAI G_wGCw'C

Cuts at:    220     265    1157    1606    1795    2491    3269    3459    4251 
   Size:         45     892     449     189     696     778     190     792

Cuts at:   4251     220 
   Size:       1446

  Fragments arranged by size: 

                1446     892     792     778     696     449     190     189
                  45
 
BsmI GAATG_Cn'

Cuts at:   1645    1645 
   Size:       5477
 
Bsp1286I G_dGCh'C

Cuts at:    191     220     247     265     289     336     406    1157    1606 
   Size:         29      27      18      24      47      70     751     449

Cuts at:   1606    1795    1963    1990    2058    2257    2470    2491    3102 
   Size:        189     168      27      68     199     213      21     611

Cuts at:   3102    3195    3269    3459    3521    4251     191 
   Size:         93      74     190      62     730    1417

  Fragments arranged by size: 

                1417     751     730     611     449     213     199     190
                 189     168      93      74      70      68      62      47
                  29      27      27      24      21      18
 
BspEI T'CCGG_A

Cuts at:   4697    4697 
   Size:       5477
 
BspHI T'CATG_A

Cuts at:   4653    4653 
   Size:       5477
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    492     638     742    1312    1891    2002    2083    2527    2741 
   Size:        146     104     570     579     111      81     444     214

Cuts at:   2741    3043    3424     492 
   Size:        302     381    2545

  Fragments arranged by size: 

                2545     579     570     444     381     302     214     146
                 111     104      81
 
BsrBI CCG'CTC

Cuts at:     50     135    3769    3823    5307    5472      50 
   Size:         85    3634      54    1484     165      55

  Fragments arranged by size: 

                3634    1484     165      85      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1206    1545    1902    3389    1206 
   Size:        339     357    1487    3294

  Fragments arranged by size: 

                3294    1487     357     339
 
BsrFI r'CCGG_y

Cuts at:   1846    3475    3656    3865    1846 
   Size:       1629     181     209    3458

  Fragments arranged by size: 

                3458    1629     209     181
 
BssSI C'ACGA_G

Cuts at:   3748    4106    3748 
   Size:        358    5119
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1745    2854    1745 
   Size:       1109    4368
 
BstBI TT'CG_AA

Cuts at:    475    3838     475 
   Size:       3363    2114
 
BstEII G'GTnAC_C

Cuts at:    783    1470     783 
   Size:        687    4790
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1044    1044 
   Size:       5477
 
BstYI r'GATC_y

Cuts at:      1    1588    2502    2969    2992    3327    3573    4585    4671 
   Size:       1587     914     467      23     335     246    1012      86

Cuts at:   4671    4683    5029       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1587    1012     914     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    121    1043    1187    1439    1800    1917     121 
   Size:        922     144     252     361     117    3681

  Fragments arranged by size: 

                3681     922     361     252     144     117
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1507    3427    3608    3771    1507 
   Size:       1920     181     163    3213

  Fragments arranged by size: 

                3213    1920     181     163
 
BtsI GCAGTG_nn'

Cuts at:    235    1006    1118    1722    2147    2720     235 
   Size:        771     112     604     425     573    2992

  Fragments arranged by size: 

                2992     771     604     573     425     112
 
DraI TTT'AAA

Cuts at:   2476    2476 
   Size:       5477
 
DrdI GACnn_nn'nnGTC

Cuts at:   2176    3183    4041    2176 
   Size:       1007     858    3612

  Fragments arranged by size: 

                3612    1007     858
 
EaeI y'GGCC_r

Cuts at:    118     130     463     599    1803    1914    3062    3236    3627 
   Size:         12     333     136    1204     111    1148     174     391

Cuts at:   3627    3654    4999     118 
   Size:         27    1345     596

  Fragments arranged by size: 

                1345    1204    1148     596     391     333     174     136
                 111      27      12
 
EagI C'GGCC_G

Cuts at:    118     130    1914    3062     118 
   Size:         12    1784    1148    2533

  Fragments arranged by size: 

                2533    1784    1148      12
 
EarI CTCTTCn'nnn_

Cuts at:   1271    2228    3500    3710    1271 
   Size:        957    1272     210    3038

  Fragments arranged by size: 

                3038    1272     957     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3685    3995    4141    5287    3685 
   Size:        310     146    1146    3875

  Fragments arranged by size: 

                3875    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    218    1155    2489     218 
   Size:        937    1334    3206

  Fragments arranged by size: 

                3206    1334     937
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    325     340     428     918    1192    1295    1359    3302    3734 
   Size:         15      88     490     274     103      64    1943     432

Cuts at:   3734    4481    4749     325 
   Size:        747     268    1053

  Fragments arranged by size: 

                1943    1053     747     490     432     274     268     103
                  88      64      15
 
EcoO109I rG'GnC_Cy

Cuts at:    187     273     386     699    1544    2253    2254    2616     187 
   Size:         86     113     313     845     709       1     362    3048

  Fragments arranged by size: 

                3048     845     709     362     313     113      86       1
            
 
EcoRI G'AATT_C

Cuts at:   2362    2481    2362 
   Size:        119    5358
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3496    3496 
   Size:       5477
 
FspI TGC'GCA

Cuts at:   3258    3258 
   Size:       5477
 
HaeII r_GCGC'y

Cuts at:    157     166    2911    3159    4181    5160     157 
   Size:          9    2745     248    1022     979     474

  Fragments arranged by size: 

                2745    1022     979     474     248       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    388     420    1039    1066    1071    1098    1747    1779    2089 
   Size:         32     619      27       5      27     649      32     310

Cuts at:   2089    2121    2422    2454    3319    3349    3351    3381     388 
   Size:         32     301      32     865      30       2      30    2484

  Fragments arranged by size: 

                2484     865     649     619     310     301      32      32
                  32      32      30      30      27      27       5       2
            
 
HincII GTy'rAC

Cuts at:   2516    2516 
   Size:       5477
 
KasI G'GCGC_C

Cuts at:   2907    3155    2907 
   Size:        248    5229
 
KpnI G_GTAC'C

Cuts at:    593    2497     593 
   Size:       1904    3573
 
MluI A'CGCG_T

Cuts at:   1870    4910    1870 
   Size:       3040    2437
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1862    4148    4332    1862 
   Size:       2286     184    3007

  Fragments arranged by size: 

                3007    2286     184
 
MscI TGG'CCA

Cuts at:    601    1805    3238     601 
   Size:       1204    1433    2840

  Fragments arranged by size: 

                2840    1433    1204
 
MslI CAynn'nnrTG

Cuts at:    493     607    1042    1715    1885    2266    3466    3593     493 
   Size:        114     435     673     170     381    1200     127    2377

  Fragments arranged by size: 

                2377    1200     673     435     381     170     127     114
            
 
MspA1I CmG'CkG

Cuts at:    123    1677    1814    1913    1919    1940    2588    2902    3262 
   Size:       1554     137      99       6      21     648     314     360

Cuts at:   3262    4275    4520     123 
   Size:       1013     245    1080

  Fragments arranged by size: 

                1554    1080    1013     648     360     314     245     137
                  99      21       6
 
NaeI GCC'GGC

Cuts at:   3658    3658 
   Size:       5477
 
NarI GG'CG_CC

Cuts at:   2908    3156    2908 
   Size:        248    5229
 
NcoI C'CATG_G

Cuts at:   1043    1187    1800    1043 
   Size:        144     613    4720

  Fragments arranged by size: 

                4720     613     144
 
NgoMIV G'CCGG_C

Cuts at:   3656    3656 
   Size:       5477
 
NotI GC'GGCC_GC

Cuts at:    118    1914     118 
   Size:       1796    3681
 
NsiI A_TGCA'T

Cuts at:    912     912 
   Size:       5477
 
NspI r_CATG'y

Cuts at:    681    1890    2530    3030    3937     681 
   Size:       1209     640     500     907    2221

  Fragments arranged by size: 

                2221    1209     907     640     500
 
PasI CC'CwG_GG

Cuts at:   1162    1162 
   Size:       5477
 
PciI A'CATG_T

Cuts at:    677    3933     677 
   Size:       3256    2221
 
PflMI CCAn_nnn'nTGG

Cuts at:    585     847     921     585 
   Size:        262      74    5141

  Fragments arranged by size: 

                5141     262      74
 
PmeI GTTT'AAAC

Cuts at:   2476    2476 
   Size:       5477
 
PmlI CAC'GTG

Cuts at:    762     762 
   Size:       5477
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4616    4648    4616 
   Size:         32    5445
 
PpuMI rG'GwC_Cy

Cuts at:   1544    1544 
   Size:       5477
 
PspOMI G'GGCC_C

Cuts at:    187    2253     187 
   Size:       2066    3411
 
PstI C_TGCA'G

Cuts at:   1672    2524    3209    1672 
   Size:        852     685    3940

  Fragments arranged by size: 

                3940     852     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5477
 
PvuII CAG'CTG

Cuts at:   1814    1940    2902    3262    1814 
   Size:        126     962     360    4029

  Fragments arranged by size: 

                4029     962     360     126
 
RsrII CG'GwC_CG

Cuts at:   3672    3672 
   Size:       5477
 
SacI G_AGCT'C

Cuts at:    220    1157    2491     220 
   Size:        937    1334    3206

  Fragments arranged by size: 

                3206    1334     937
 
SacII CC_GC'GG

Cuts at:    124    1920     124 
   Size:       1796    3681
 
SalI G'TCGA_C

Cuts at:   2514    2514 
   Size:       5477
 
SapI GCTCTTCn'nnn_

Cuts at:   2228    3500    3710    2228 
   Size:       1272     210    3995

  Fragments arranged by size: 

                3995    1272     210
 
SbfI CC_TGCA'GG

Cuts at:   2524    2524 
   Size:       5477
 
ScaI AGT'ACT

Cuts at:    374    1744    2191    4994     374 
   Size:       1370     447    2803     857

  Fragments arranged by size: 

                2803    1370     857     447
 
SexAI A'CCwGG_T

Cuts at:    779     779 
   Size:       5477
 
SfcI C'TryA_G

Cuts at:     33     872    1146    1668    2520    3205    4198    4389      33 
   Size:        839     274     522     852     685     993     191    1121

  Fragments arranged by size: 

                1121     993     852     839     685     522     274     191
            
 
SfoI GGC'GCC

Cuts at:   2909    3157    2909 
   Size:        248    5229
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5477
 
SmaI CCC'GGG

Cuts at:   1651    2499    1651 
   Size:        848    4629
 
SmlI C'TyrA_G

Cuts at:    428    1333    1351    2119    2632    3547    4039    4301     428 
   Size:        905      18     768     513     915     492     262    1604

  Fragments arranged by size: 

                1604     915     905     768     513     492     262      18
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5477
 
SphI G_CATG'C

Cuts at:   2530    3030    2530 
   Size:        500    4977
 
StyI C'CwwG_G

Cuts at:   1043    1187    1388    1800    2249    2611    2965    1043 
   Size:        144     201     412     449     362     354    3555

  Fragments arranged by size: 

                3555     449     412     362     354     201     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    619    2369    3060    3193    3652    3861     619 
   Size:       1750     691     133     459     209    2235

  Fragments arranged by size: 

                2235    1750     691     459     209     133
 
TatI w'GTAC_w

Cuts at:    372    1742    2189    4992     372 
   Size:       1370     447    2803     857

  Fragments arranged by size: 

                2803    1370     857     447
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    443     475     553     585    1352    1384    1813    1845    4994 
   Size:         32      78      32     767      32     429      32    3149

Cuts at:   4994    5026     443 
   Size:         32     894

  Fragments arranged by size: 

                3149     894     767     429      78      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2173    3274    2173 
   Size:       1101    4376
 
XbaI T'CTAG_A

Cuts at:   2508    2508 
   Size:       5477
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1437    1813    2223    2972    1437 
   Size:        376     410     749    3942

  Fragments arranged by size: 

                3942     749     410     376
 
XmaI C'CCGG_G

Cuts at:   1649    2497    1649 
   Size:        848    4629
 
XmnI GAAnn'nnTTC

Cuts at:    481     481 
   Size:       5477
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NsiI     NspI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AscI    AvrII     BmtI    BsiWI    BsmBI
    BsrGI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NruI     PacI     PfoI    PshAI     PsiI    PspXI     PsrI    SanDI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     ZraI
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