Restriction map for pF1KB5616
 (Linear) (Six-base) MAPSORT of: KIBB5616.seq  Check: 7511  from: 1  to: 2364



KIBB5616.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     384     634    1204    1783    1894    2364 
   Size:        384     250     570     579     111     470

  Fragments arranged by size: 

                 579     570     470     384     250     111
 
AccI GT'mk_AC

Cuts at:      0    2332    2364 
   Size:       2332      32
 
Acc65I G'GTAC_C

Cuts at:      0     481    2364 
   Size:        481    1883
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     232    1187    1251    2364 
   Size:        217      15     955      64    1113

  Fragments arranged by size: 

                1113     955     217      64      15
 
AflII C'TTAA_G

Cuts at:      0    2011    2364 
   Size:       2011     353
 
AflIII A'CryG_T

Cuts at:      0     569     651    1762    2364 
   Size:        569      82    1111     602

  Fragments arranged by size: 

                1111     602     569      82
 
AleI CACnn'nnGTG

Cuts at:      0    1607    2364 
   Size:       1607     757
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1810    2364 
   Size:       1810     554
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1116    1148    2364 
   Size:       1116      32    1216

  Fragments arranged by size: 

                1216    1116      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     126     477    1009    2364 
   Size:        126     351     532    1355

  Fragments arranged by size: 

                1355     532     351     126
 
ApaI G_GGCC'C

Cuts at:      0      83    2149    2364 
   Size:         83    2066     215

  Fragments arranged by size: 

                2066     215      83
 
ApoI r'AATT_y

Cuts at:      0     372    2254    2263    2364 
   Size:        372    1882       9     101

  Fragments arranged by size: 

                1882     372     101       9
 
AvaI C'yCGr_G

Cuts at:      0     260    1541    2364 
   Size:        260    1281     823

  Fragments arranged by size: 

                1281     823     260
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      75     108     465     498    2364 
   Size:         75      33     357      33    1866

  Fragments arranged by size: 

                1866     357      75      33      33
 
BamHI G'GATC_C

Cuts at:      0    1480    2364 
   Size:       1480     884
 
BanI G'GyrC_C

Cuts at:      0     481    2364 
   Size:        481    1883
 
BanII G_rGCy'C

Cuts at:      0      83     112     139     181     298    1049    1855    1950 
   Size:         83      29      27      42     117     751     806      95

Cuts at:   1950    2149    2364 
   Size:        199     215

  Fragments arranged by size: 

                 806     751     215     199     117      95      83      42
                  29      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     625    2364 
   Size:        625    1739
 
BbvCI CC'TCA_GC

Cuts at:      0     283    2364 
   Size:        283    2081
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1852    2364 
   Size:       1852     512
 
BfrBI ATG'CAT

Cuts at:      0     802    2364 
   Size:        802    1562
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1390    1473    1571    2364 
   Size:       1390      83      98     793

  Fragments arranged by size: 

                1390     793      98      83
 
Bme1580I G_kGCm'C

Cuts at:      0      83     228    1882    2149    2362    2364 
   Size:         83     145    1654     267     213       2

  Fragments arranged by size: 

                1654     267     213     145      83       2
 
BmgBI CAC'GTC

Cuts at:      0    2330    2364 
   Size:       2330      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     241    1479    2364 
   Size:        241    1238     885

  Fragments arranged by size: 

                1238     885     241
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1701    2364 
   Size:       1701     663
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     810    1084    2364 
   Size:        320     490     274    1280

  Fragments arranged by size: 

                1280     490     320     274
 
Bpu10I CC'TnA_GC

Cuts at:      0     283    1686    2364 
   Size:        283    1403     678

  Fragments arranged by size: 

                1403     678     283
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305    1210    1228    2364 
   Size:        305     905      18    1136

  Fragments arranged by size: 

                1136     905     305      18
 
BsaAI yAC'GTr

Cuts at:      0     654    2351    2364 
   Size:        654    1697      13

  Fragments arranged by size: 

                1697     654      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     335     365     910     940    1244    1274    1639    1669 
   Size:        335      30     545      30     304      30     365      30

Cuts at:   1669    2364 
   Size:        695

  Fragments arranged by size: 

                 695     545     365     335     304      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1516    2218    2364 
   Size:       1516     702     146

  Fragments arranged by size: 

                1516     702     146
 
BseYI C'CCAG_C

Cuts at:      0      48    1476    1520    2364 
   Size:         48    1428      44     844

  Fragments arranged by size: 

                1428     844      48      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1722    1821    1884    2364 
   Size:       1722      99      63     480

  Fragments arranged by size: 

                1722     480      99      63
 
BsiEI CG_ry'CG

Cuts at:      0      13      25    1809    2247    2364 
   Size:         13      12    1784     438     117

  Fragments arranged by size: 

                1784     438     117      13      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     112     157    1049    1498    1687    2364 
   Size:        112      45     892     449     189     677

  Fragments arranged by size: 

                 892     677     449     189     112      45
 
BsmI GAATG_Cn'

Cuts at:      0    1537    2364 
   Size:       1537     827
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     112     139     157     181     228     298    1049 
   Size:         83      29      27      18      24      47      70     751

Cuts at:   1049    1498    1687    1855    1882    1950    2149    2362    2364 
   Size:        449     189     168      27      68     199     213       2

  Fragments arranged by size: 

                 751     449     213     199     189     168      83      70
                  68      47      29      27      27      24      18       2
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     384     530     634    1204    1783    1894    1975    2364 
   Size:        384     146     104     570     579     111      81     389

  Fragments arranged by size: 

                 579     570     389     384     146     111     104      81
            
 
BsrBI CCG'CTC

Cuts at:      0      27    2364 
   Size:         27    2337
 
BsrDI GCAATG_nn'

Cuts at:      0    1098    1437    1794    2364 
   Size:       1098     339     357     570

  Fragments arranged by size: 

                1098     570     357     339
 
BsrFI r'CCGG_y

Cuts at:      0    1738    2364 
   Size:       1738     626
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1637    2364 
   Size:       1637     727
 
BstBI TT'CG_AA

Cuts at:      0     367    2364 
   Size:        367    1997
 
BstEII G'GTnAC_C

Cuts at:      0     675    1362    2364 
   Size:        675     687    1002

  Fragments arranged by size: 

                1002     687     675
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     936    2364 
   Size:        936    1428
 
BstYI r'GATC_y

Cuts at:      0    1480    2364 
   Size:       1480     884
 
BtgI C'CryG_G

Cuts at:      0      13     935    1079    1331    1692    1809    2364 
   Size:         13     922     144     252     361     117     555

  Fragments arranged by size: 

                 922     555     361     252     144     117      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1399    2364 
   Size:       1399     965
 
BtsI GCAGTG_nn'

Cuts at:      0     127     898    1010    1614    2039    2364 
   Size:        127     771     112     604     425     325

  Fragments arranged by size: 

                 771     604     425     325     127     112
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2068    2364 
   Size:       2068     296
 
EaeI y'GGCC_r

Cuts at:      0      10      22     355     491    1695    1806    2364 
   Size:         10      12     333     136    1204     111     558

  Fragments arranged by size: 

                1204     558     333     136     111      12      10
 
EagI C'GGCC_G

Cuts at:      0      10      22    1806    2364 
   Size:         10      12    1784     558

  Fragments arranged by size: 

                1784     558      12      10
 
EarI CTCTTCn'nnn_

Cuts at:      0    1163    2120    2364 
   Size:       1163     957     244

  Fragments arranged by size: 

                1163     957     244
 
EcoICRI GAG'CTC

Cuts at:      0     110    1047    2364 
   Size:        110     937    1317

  Fragments arranged by size: 

                1317     937     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     232     320     810    1084    1187    1251    2364 
   Size:        217      15      88     490     274     103      64    1113

  Fragments arranged by size: 

                1113     490     274     217     103      88      64      15
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      79     165     278     591    1436    2145    2146    2364 
   Size:         79      86     113     313     845     709       1     218

  Fragments arranged by size: 

                 845     709     313     218     113      86      79       1
            
 
EcoRI G'AATT_C

Cuts at:      0    2254    2364 
   Size:       2254     110
 
HaeII r_GCGC'y

Cuts at:      0      49      58    2364 
   Size:         49       9    2306

  Fragments arranged by size: 

                2306      49       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     312     931     958     963     990    1639    1671 
   Size:        280      32     619      27       5      27     649      32

Cuts at:   1671    1981    2013    2314    2346    2364 
   Size:        310      32     301      32      18

  Fragments arranged by size: 

                 649     619     310     301     280      32      32      32
                  32      27      27      18       5
 
KpnI G_GTAC'C

Cuts at:      0     485    2364 
   Size:        485    1879
 
MluI A'CGCG_T

Cuts at:      0    1762    2364 
   Size:       1762     602
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1754    2364 
   Size:       1754     610
 
MscI TGG'CCA

Cuts at:      0     493    1697    2364 
   Size:        493    1204     667

  Fragments arranged by size: 

                1204     667     493
 
MslI CAynn'nnrTG

Cuts at:      0     385     499     934    1607    1777    2158    2364 
   Size:        385     114     435     673     170     381     206

  Fragments arranged by size: 

                 673     435     385     381     206     170     114
 
MspA1I CmG'CkG

Cuts at:      0      15    1569    1706    1805    1811    1832    2364 
   Size:         15    1554     137      99       6      21     532

  Fragments arranged by size: 

                1554     532     137      99      21      15       6
 
NcoI C'CATG_G

Cuts at:      0     935    1079    1692    2364 
   Size:        935     144     613     672

  Fragments arranged by size: 

                 935     672     613     144
 
NotI GC'GGCC_GC

Cuts at:      0      10    1806    2364 
   Size:         10    1796     558

  Fragments arranged by size: 

                1796     558      10
 
NsiI A_TGCA'T

Cuts at:      0     804    2364 
   Size:        804    1560
 
NspI r_CATG'y

Cuts at:      0     573    1782    2364 
   Size:        573    1209     582

  Fragments arranged by size: 

                1209     582     573
 
PasI CC'CwG_GG

Cuts at:      0    1054    2364 
   Size:       1054    1310
 
PciI A'CATG_T

Cuts at:      0     569    2364 
   Size:        569    1795
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     477     739     813    2364 
   Size:        477     262      74    1551

  Fragments arranged by size: 

                1551     477     262      74
 
PmlI CAC'GTG

Cuts at:      0     654    2364 
   Size:        654    1710
 
PpuMI rG'GwC_Cy

Cuts at:      0    1436    2364 
   Size:       1436     928
 
PspOMI G'GGCC_C

Cuts at:      0      79    2145    2364 
   Size:         79    2066     219

  Fragments arranged by size: 

                2066     219      79
 
PstI C_TGCA'G

Cuts at:      0    1564    2364 
   Size:       1564     800
 
PvuII CAG'CTG

Cuts at:      0    1706    1832    2364 
   Size:       1706     126     532

  Fragments arranged by size: 

                1706     532     126
 
SacI G_AGCT'C

Cuts at:      0     112    1049    2364 
   Size:        112     937    1315

  Fragments arranged by size: 

                1315     937     112
 
SacII CC_GC'GG

Cuts at:      0      16    1812    2364 
   Size:         16    1796     552

  Fragments arranged by size: 

                1796     552      16
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2120    2364 
   Size:       2120     244
 
ScaI AGT'ACT

Cuts at:      0     266    1636    2083    2364 
   Size:        266    1370     447     281

  Fragments arranged by size: 

                1370     447     281     266
 
SexAI A'CCwGG_T

Cuts at:      0     671    2364 
   Size:        671    1693
 
SfcI C'TryA_G

Cuts at:      0     764    1038    1560    2364 
   Size:        764     274     522     804

  Fragments arranged by size: 

                 804     764     522     274
 
SmaI CCC'GGG

Cuts at:      0    1543    2364 
   Size:       1543     821
 
SmlI C'TyrA_G

Cuts at:      0     320    1225    1243    2011    2364 
   Size:        320     905      18     768     353

  Fragments arranged by size: 

                 905     768     353     320      18
 
StyI C'CwwG_G

Cuts at:      0     935    1079    1280    1692    2141    2364 
   Size:        935     144     201     412     449     223

  Fragments arranged by size: 

                 935     449     412     223     201     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     511    2261    2364 
   Size:        511    1750     103

  Fragments arranged by size: 

                1750     511     103
 
TatI w'GTAC_w

Cuts at:      0     264    1634    2081    2364 
   Size:        264    1370     447     283

  Fragments arranged by size: 

                1370     447     283     264
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     335     367     445     477    1244    1276    1705    1737 
   Size:        335      32      78      32     767      32     429      32

Cuts at:   1737    2364 
   Size:        627

  Fragments arranged by size: 

                 767     627     429     335      78      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2065    2364 
   Size:       2065     299
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1329    1705    2115    2364 
   Size:       1329     376     410     249

  Fragments arranged by size: 

                1329     410     376     249
 
XmaI C'CCGG_G

Cuts at:      0    1541    2364 
   Size:       1541     823
 
XmnI GAAnn'nnTTC

Cuts at:      0     373    2364 
   Size:        373    1991
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbsI    BbvCI    BciVI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NotI     NsiI     NspI     PasI     PciI
    PflMI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII
     SapI     ScaI    SexAI     SfcI     SmaI     SmlI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AgeI     AhdI    ApaLI     AscI     AseI
    AsiSI    AvrII     BbeI     BcgI     BclI    BglII     BlpI     BmtI
     BsaI    BsaBI    BsaHI    BsaWI    BsiWI    BsmBI    BspEI    BspHI
    BsrGI   BssHII    BssSI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     EciI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     MfeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NruI     PacI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SbfI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     XbaI     XhoI     ZraI
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