Restriction map for pF1KB4713
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BbvCI       BciVI       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    EcoO65I     EcoRV       
EcoT22I     FspI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
SalI        SbfI        ScaI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII       1168  TCCGGA               T^CCGGA
AflII        2059  CTTAAG               C^TTAAG
AspHI        1393  GAGCTC               G[AT]GC[AT]^C
AspI          937  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI          15  GGATCC               G^GATCC
BbrPI        1306  CACGTG               CAC^GTG
BcgI         1518  CGAGGAGCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1067  TGATCA               T^GATCA
BfaI         1717  CTAG                 C^TAG
BfrI         2059  CTTAAG               C^TTAAG
BglII         539  AGATCT               A^GATCT
BsaWI        1168  TCCGGA               [AT]^CCGG[AT]
BseAI        1168  TCCGGA               T^CCGGA
BsgI         1942  GTGCAG               GTGCAG(16/14)
BsiEI        1123  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1393  GAGCTC               G[AT]GC[AT]^C
BspEI        1168  TCCGGA               T^CCGGA
BspMI         247  GCAGGT               ACCTGC(4/8)
CpoI         1170  CGGACCG              CG^G[AT]CCG
EagI         1123  CGGCCG               C^GGCCG
EclXI        1123  CGGCCG               C^GGCCG
Eco52I       1123  CGGCCG               C^GGCCG
EcoRI         374  GAATTC               G^AATTC
FbaI         1067  TGATCA               T^GATCA
FseI           42  GGCCGGCC             GGCCGG^CC
KspI         1265  CCGCGG               CCGC^GG
MaeI         1717  CTAG                 C^TAG
MroI         1168  TCCGGA               T^CCGGA
PaeR7I       1671  CTCGAG               C^TCGAG
PflFI         937  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1306  CACGTG               CAC^GTG
PmlI         1306  CACGTG               CAC^GTG
PshAI        1503  GACTGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1135  CAGCTG               CAG^CTG
RsrII        1170  CGGACCG              CG^G[AT]CCG
SacI         1393  GAGCTC               GAGCT^C
SacII        1265  CCGCGG               CCGC^GG
SexAI        2093  ACCTGGT              A^CC[AT]GGT
SfiI         1872  GGCCTACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         116  CGCCGGCG             C[GA]^CCGG[CT]G
Tth111I       937  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          140  CCAGCCCGGGGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1671  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          374  GAATTC               [GA]^AATT[CT]
             2110  AAATTT              
AlwNI         725  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2089  CAGGACCTG           
ApoI          374  GAATTC               [GA]^AATT[CT]
             2110  AAATTT              
BalI          393  TGGCCA               TGG^CCA
             1302  TGGCCA              
BpmI         1613  CTGGAG               CTGGAG(16/14)
             1627  CTGGAG              
BsaAI        1306  CACGTG               [CT]AC^GT[GA]
             2044  TACGTG              
BsmBI        1616  GAGACG               CGTCTC(1/5)
             1636  GAGACG              
BssSI        1201  CACGAG               CACGAG(-5/-1)
              473  CTCGTG              
MluNI         393  TGGCCA               TGG^CCA
             1302  TGGCCA              
MscI          393  TGGCCA               TGG^CCA
             1302  TGGCCA              
SapI         1833  GCTCTTC              GCTCTTC(1/4)
             2076  GCTCTTC             
StuI          575  AGGCCT               AGG^CCT
             1973  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AocI        ApaI        AvaI        AvaII       
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BglI        BmyI        BpuAI       BsaHI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmaCI       XmaI        
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