Restriction map for
pF1KB4713
(Linear) (Six-base) MAPSORT of: KIBB4713.seq Check: 4819 from: 1 to: 2328
KIBB4713.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 238 2328
Size: 238 2090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 719 824 989 1682 2328
Size: 364 355 105 165 693 646
Fragments arranged by size:
693 646 364 355 165 105
AflII C'TTAA_G
Cuts at: 0 2059 2328
Size: 2059 269
AleI CACnn'nnGTG
Cuts at: 0 241 529 2328
Size: 241 288 1799
Fragments arranged by size:
1799 288 241
AlwNI CAG_nnn'CTG
Cuts at: 0 730 2094 2328
Size: 730 1364 234
Fragments arranged by size:
1364 730 234
ApaI G_GGCC'C
Cuts at: 0 165 199 1708 2328
Size: 165 34 1509 620
Fragments arranged by size:
1509 620 165 34
ApoI r'AATT_y
Cuts at: 0 374 2110 2328
Size: 374 1736 218
Fragments arranged by size:
1736 374 218
AvaI C'yCGr_G
Cuts at: 0 49 144 164 192 604 637 1150 1600
Size: 49 95 20 28 412 33 513 450
Cuts at: 1600 1671 2328
Size: 71 657
Fragments arranged by size:
657 513 450 412 95 71 49 33
28 20
BamHI G'GATC_C
Cuts at: 0 15 2328
Size: 15 2313
BanI G'GyrC_C
Cuts at: 0 68 120 154 1726 2328
Size: 68 52 34 1572 602
Fragments arranged by size:
1572 602 68 52 34
BanII G_rGCy'C
Cuts at: 0 140 165 199 418 822 1397 1441 1589
Size: 140 25 34 219 404 575 44 148
Cuts at: 1589 1626 1708 2328
Size: 37 82 620
Fragments arranged by size:
620 575 404 219 148 140 82 44
37 34 25
BbeI G_GCGC'C
Cuts at: 0 124 158 1730 2328
Size: 124 34 1572 598
Fragments arranged by size:
1572 598 124 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 901 977 1673 2328
Size: 901 76 696 655
Fragments arranged by size:
901 696 655 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1507 1541 2328
Size: 1507 34 787
Fragments arranged by size:
1507 787 34
BclI T'GATC_A
Cuts at: 0 1067 2328
Size: 1067 1261
BglI GCCn_nnn'nGGC
Cuts at: 0 118 1310 1423 1536 1541 1879 2328
Size: 118 1192 113 113 5 338 449
Fragments arranged by size:
1192 449 338 118 113 113 5
BglII A'GATC_T
Cuts at: 0 539 2328
Size: 539 1789
Bme1580I G_kGCm'C
Cuts at: 0 165 199 1708 2328
Size: 165 34 1509 620
Fragments arranged by size:
1509 620 165 34
BmgBI CAC'GTC
Cuts at: 0 399 2328
Size: 399 1929
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 1842 2328
Size: 51 1791 486
Fragments arranged by size:
1791 486 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1473 2328
Size: 1473 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1634 1648 2328
Size: 1634 14 680
Fragments arranged by size:
1634 680 14
Bpu10I CC'TnA_GC
Cuts at: 0 64 2328
Size: 64 2264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2008 2328
Size: 2008 320
BsaAI yAC'GTr
Cuts at: 0 1308 2046 2328
Size: 1308 738 282
Fragments arranged by size:
1308 738 282
BsaHI Gr'CG_yC
Cuts at: 0 100 121 155 175 1415 1508 1639 1727
Size: 100 21 34 20 1240 93 131 88
Cuts at: 1727 2328
Size: 601
Fragments arranged by size:
1240 601 131 100 93 88 34 21
20
BsaWI w'CCGG_w
Cuts at: 0 1168 2328
Size: 1168 1160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 765 795 2328
Size: 765 30 1533
Fragments arranged by size:
1533 765 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 622 812 1381 1410 1447 1513 1534
Size: 436 186 190 569 29 37 66 21
Cuts at: 1534 2085 2328
Size: 551 243
Fragments arranged by size:
569 551 436 243 190 186 66 37
29 21
BseYI C'CCAG_C
Cuts at: 0 130 139 426 1186 1474 1589 2328
Size: 130 9 287 760 288 115 739
Fragments arranged by size:
760 739 288 287 130 115 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1963 2328
Size: 1963 365
BsiEI CG_ry'CG
Cuts at: 0 1126 2328
Size: 1126 1202
BsiHKAI G_wGCw'C
Cuts at: 0 1397 2328
Size: 1397 931
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1610 1630 2328
Size: 1610 20 698
Fragments arranged by size:
1610 698 20
Bsp1286I G_dGCh'C
Cuts at: 0 140 165 199 418 822 1397 1441 1589
Size: 140 25 34 219 404 575 44 148
Cuts at: 1589 1626 1708 2328
Size: 37 82 620
Fragments arranged by size:
620 575 404 219 148 140 82 44
37 34 25
BspEI T'CCGG_A
Cuts at: 0 1168 2328
Size: 1168 1160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 238 2328
Size: 238 2090
BsrFI r'CCGG_y
Cuts at: 0 43 117 187 1125 1535 2328
Size: 43 74 70 938 410 793
Fragments arranged by size:
938 793 410 74 70 43
BssSI C'ACGA_G
Cuts at: 0 473 1201 2328
Size: 473 728 1127
Fragments arranged by size:
1127 728 473
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 264 1194 1482 2328
Size: 243 21 930 288 846
Fragments arranged by size:
930 846 288 243 21
BstYI r'GATC_y
Cuts at: 0 15 539 985 2328
Size: 15 524 446 1343
Fragments arranged by size:
1343 524 446 15
Bsu36I CC'TnA_GG
Cuts at: 0 347 419 1284 1360 2328
Size: 347 72 865 76 968
Fragments arranged by size:
968 865 347 76 72
BtgI C'CryG_G
Cuts at: 0 221 263 524 1120 1259 1265 1651 2328
Size: 221 42 261 596 139 6 386 677
Fragments arranged by size:
677 596 386 261 221 139 42 6
BtsI GCAGTG_nn'
Cuts at: 0 1738 1946 2328
Size: 1738 208 382
Fragments arranged by size:
1738 382 208
EaeI y'GGCC_r
Cuts at: 0 2 41 393 1123 1139 1262 1302 1547
Size: 2 39 352 730 16 123 40 245
Cuts at: 1547 1694 1886 2328
Size: 147 192 442
Fragments arranged by size:
730 442 352 245 192 147 123 40
39 16 2
EagI C'GGCC_G
Cuts at: 0 1123 2328
Size: 1123 1205
EarI CTCTTCn'nnn_
Cuts at: 0 1840 2083 2158 2328
Size: 1840 243 75 170
Fragments arranged by size:
1840 243 170 75
EcoICRI GAG'CTC
Cuts at: 0 1395 2328
Size: 1395 933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 719 824 989 1634 1648 1682 2328
Size: 364 355 105 165 645 14 34 646
Fragments arranged by size:
646 645 364 355 165 105 34 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1363 2002 2086 2328
Size: 1363 639 84 242
Fragments arranged by size:
1363 639 242 84
EcoO109I rG'GnC_Cy
Cuts at: 0 53 161 196 1254 1542 1704 1705 1720
Size: 53 108 35 1058 288 162 1 15
Cuts at: 1720 2091 2328
Size: 371 237
Fragments arranged by size:
1058 371 288 237 162 108 53 35
15 1
EcoRI G'AATT_C
Cuts at: 0 374 2328
Size: 374 1954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1995 2328
Size: 1995 333
FseI GG_CCGG'CC
Cuts at: 0 47 2328
Size: 47 2281
HaeII r_GCGC'y
Cuts at: 0 124 158 1481 1730 2150 2312 2328
Size: 124 34 1323 249 420 162 16
Fragments arranged by size:
1323 420 249 162 124 34 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 784 816 1231 1263 1405 1437
Size: 456 32 296 32 415 32 142 32
Cuts at: 1437 1561 1593 2328
Size: 124 32 735
Fragments arranged by size:
735 456 415 296 142 124 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 120 154 1726 2328
Size: 120 34 1572 602
Fragments arranged by size:
1572 602 120 34
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1703 2328
Size: 1703 625
MscI TGG'CCA
Cuts at: 0 395 1304 2328
Size: 395 909 1024
Fragments arranged by size:
1024 909 395
MslI CAynn'nnrTG
Cuts at: 0 241 529 2182 2270 2328
Size: 241 288 1653 88 58
Fragments arranged by size:
1653 288 241 88 58
MspA1I CmG'CkG
Cuts at: 0 39 1137 1267 2328
Size: 39 1098 130 1061
Fragments arranged by size:
1098 1061 130 39
NaeI GCC'GGC
Cuts at: 0 45 119 189 1537 2328
Size: 45 74 70 1348 791
Fragments arranged by size:
1348 791 74 70 45
NarI GG'CG_CC
Cuts at: 0 121 155 1727 2328
Size: 121 34 1572 601
Fragments arranged by size:
1572 601 121 34
NcoI C'CATG_G
Cuts at: 0 221 263 1259 2328
Size: 221 42 996 1069
Fragments arranged by size:
1069 996 221 42
NgoMIV G'CCGG_C
Cuts at: 0 43 117 187 1535 2328
Size: 43 74 70 1348 793
Fragments arranged by size:
1348 793 74 70 43
PasI CC'CwG_GG
Cuts at: 0 1035 1708 2328
Size: 1035 673 620
Fragments arranged by size:
1035 673 620
PflMI CCAn_nnn'nTGG
Cuts at: 0 762 1006 2094 2247 2328
Size: 762 244 1088 153 81
Fragments arranged by size:
1088 762 244 153 81
PfoI T'CCnGG_A
Cuts at: 0 1493 2087 2328
Size: 1493 594 241
Fragments arranged by size:
1493 594 241
PmlI CAC'GTG
Cuts at: 0 1308 2328
Size: 1308 1020
PpuMI rG'GwC_Cy
Cuts at: 0 53 1254 1720 2091 2328
Size: 53 1201 466 371 237
Fragments arranged by size:
1201 466 371 237 53
PshAI GACnn'nnGTC
Cuts at: 0 1507 2328
Size: 1507 821
PspOMI G'GGCC_C
Cuts at: 0 161 195 1704 2328
Size: 161 34 1509 624
Fragments arranged by size:
1509 624 161 34
PspXI vC'TCGA_Gb
Cuts at: 0 1671 2328
Size: 1671 657
PstI C_TGCA'G
Cuts at: 0 519 1029 2039 2328
Size: 519 510 1010 289
Fragments arranged by size:
1010 519 510 289
PvuII CAG'CTG
Cuts at: 0 1137 2328
Size: 1137 1191
RsrII CG'GwC_CG
Cuts at: 0 1171 2328
Size: 1171 1157
SacI G_AGCT'C
Cuts at: 0 1397 2328
Size: 1397 931
SacII CC_GC'GG
Cuts at: 0 1268 2328
Size: 1268 1060
SanDI GG'GwC_CC
Cuts at: 0 53 2328
Size: 53 2275
SapI GCTCTTCn'nnn_
Cuts at: 0 1840 2083 2328
Size: 1840 243 245
Fragments arranged by size:
1840 245 243
SexAI A'CCwGG_T
Cuts at: 0 2093 2328
Size: 2093 235
SfcI C'TryA_G
Cuts at: 0 515 1025 2035 2207 2328
Size: 515 510 1010 172 121
Fragments arranged by size:
1010 515 510 172 121
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1879 2328
Size: 1879 449
SfoI GGC'GCC
Cuts at: 0 122 156 1728 2328
Size: 122 34 1572 600
Fragments arranged by size:
1572 600 122 34
SgrAI Cr'CCGG_yG
Cuts at: 0 117 2328
Size: 117 2211
SmaI CCC'GGG
Cuts at: 0 51 146 166 2328
Size: 51 95 20 2162
Fragments arranged by size:
2162 95 51 20
SmlI C'TyrA_G
Cuts at: 0 1671 1987 2059 2328
Size: 1671 316 72 269
Fragments arranged by size:
1671 316 269 72
StuI AGG'CCT
Cuts at: 0 577 1975 2328
Size: 577 1398 353
Fragments arranged by size:
1398 577 353
StyI C'CwwG_G
Cuts at: 0 221 263 1259 2328
Size: 221 42 996 1069
Fragments arranged by size:
1069 996 221 42
TatI w'GTAC_w
Cuts at: 0 1349 2021 2328
Size: 1349 672 307
Fragments arranged by size:
1349 672 307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 765 797 2328
Size: 292 32 441 32 1531
Fragments arranged by size:
1531 441 292 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 940 2328
Size: 940 1388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 2328
Size: 147 2181
XhoI C'TCGA_G
Cuts at: 0 1671 2328
Size: 1671 657
XmaI C'CCGG_G
Cuts at: 0 49 144 164 2328
Size: 49 95 20 2164
Fragments arranged by size:
2164 95 49 20
Enzymes that do cut:
AarI AcuI AflII AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BclI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BssSI BstXI
BstYI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PstI PvuII RsrII SacI SacII SanDI SapI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StuI
StyI TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BfrBI BlpI BmtI BsaI BsaBI BsiWI
BsmI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoRV FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PsiI PsrI PvuI SalI
SbfI ScaI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TaqII XbaI XmnI ZraI