Restriction map for pF1KB7274
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BlnI        BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HpaII       KasI        
KspI        MfeI        MluI        MroI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        926  GGTACC               G^GTACC
Alw44I       1139  GTGCAC               G^TGCAC
AlwNI        1694  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1139  GTGCAC               G^TGCAC
Asp718I       926  GGTACC               G^GTACC
AspHI        1139  GTGCAC               G[AT]GC[AT]^C
BalI          841  TGGCCA               TGG^CCA
BanI          926  GGTACC               G^G[CT][GA]CC
BbsI         1626  GAAGAC               GAAGAC(2/6)
BciVI         224  GGATAC               GTATCC(6/5)
BglII        1879  AGATCT               A^GATCT
BlpI           80  GCTTAGC              GC^T[ACGT]AGC
BmyI         1139  GTGCAC               G[AGT]GC[ACT]^C
Bpu1102I       80  GCTTAGC              GC^T[ACGT]AGC
BpuAI        1626  GAAGAC               GAAGAC(2/6)
BsaAI         975  CACGTA               [CT]AC^GT[GA]
BsaBI         541  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1833  GGTCTC               GGTCTC(1/5)
BseRI        1015  GAGGAG               GAGGAG(10/8)
BsiHKAI      1139  GTGCAC               G[AT]GC[AT]^C
Bsp1286I     1139  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      804  TGTACA               T^GTACA
BspLU11I      366  ACATGT               A^CATGT
BsrDI        1453  GCAATG               GCAATG(2/0)
BsrGI         804  TGTACA               T^GTACA
BstEII        615  GGTAACC              G^GT[ACGT]ACC
BstPI         615  GGTAACC              G^GT[ACGT]ACC
CelII          80  GCTTAGC              GC^T[ACGT]AGC
CfoI          177  GCGC                 GCG^C
DraI         1327  TTTAAA               TTT^AAA
DraII         899  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1302  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          841  TGGCCA               [CT]^GGCC[GA]
EcoNI        1933  CCTATAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      899  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       615  GGTAACC              G^GT[ACGT]ACC
EcoRI        2208  GAATTC               G^AATTC
EcoRV        2167  GATATC               GAT^ATC
EcoT22I      1456  ATGCAT               ATGCA^T
HhaI          177  GCGC                 GCG^C
HinP1I        177  GCGC                 G^CGC
HincII        663  GTTAAC               GT[CT]^[GA]AC
HindII        663  GTTAAC               GT[CT]^[GA]AC
HindIII      1209  AAGCTT               A^AGCTT
HpaI          663  GTTAAC               GTT^AAC
KpnI          926  GGTACC               GGTAC^C
MamI          541  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         841  TGGCCA               TGG^CCA
MscI          841  TGGCCA               TGG^CCA
MslI         1596  CATGTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI         1456  ATGCAT               ATGCA^T
PpuMI         899  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII        1682  CAGCTG               CAG^CTG
SexAI         209  ACCTGGT              A^CC[AT]GGT
SpeI         1615  ACTAGT               A^CTAGT
SspBI         804  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           43  CCGC                 CCGC(-3/-1)
             2119  GCGG                
AflIII        366  ACATGT               A^C[GA][CT]GT
             1855  ACACGT              
AvaII         900  GGACC                G^G[AT]CC
             1073  GGTCC               
BpmI         1679  CTCCAG               CTGGAG(16/14)
             1836  CTCCAG              
BsmFI         899  GGGAC                GGGAC(10/14)
             1979  GTCCC               
Cac8I         770  GCCAGC               GC[ACGT]^[ACGT]GC
             1407  GCAAGC              
EcoT14I        92  CCAAGG               C^C[AT][AT]GG
              904  CCAAGG              
MaeII         976  ACGT                 A^CGT
             1857  ACGT                
MspA1I       1682  CAGCTG               C[AC]G^C[GT]G
             2117  CAGCGG              
PleI         1943  GAGTC                GAGTC(4/5)
             1977  GAGTC               
PstI          172  CTGCAG               CTGCA^G
              523  CTGCAG              
StyI           92  CCAAGG               C^C[AT][AT]GG
              904  CCAAGG              
Tsp45I        380  GTGAC                ^GT[GC]AC
             1702  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     BbvI        BfaI        BsaJI       BsiYI       
BslI        BsmAI       BsmI        BsrI        BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com