Restriction map for pF1KB7274
 (Linear) (Six-base) MAPSORT of: KIBB7274.seq  Check: 5762  from: 1  to: 2235



KIBB7274.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

Acc65I G'GTAC_C

Cuts at:      0     926    2235 
   Size:        926    1309
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     642     890    1489    1742    1868    1886    2235 
   Size:        557      85     248     599     253     126      18     349

  Fragments arranged by size: 

                 599     557     349     253     248     126      85      18
            
 
AflIII A'CryG_T

Cuts at:      0     366    1855    2235 
   Size:        366    1489     380

  Fragments arranged by size: 

                1489     380     366
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     783    1618    2235 
   Size:        783     835     617

  Fragments arranged by size: 

                 835     783     617
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1699    2235 
   Size:       1699     536
 
ApaLI G'TGCA_C

Cuts at:      0    1139    2235 
   Size:       1139    1096
 
ApoI r'AATT_y

Cuts at:      0     287     573     626     811    1049    1225    1294    1743 
   Size:        287     286      53     185     238     176      69     449

Cuts at:   1743    2208    2235 
   Size:        465      27

  Fragments arranged by size: 

                 465     449     287     286     238     185     176      69
                  53      27
 
BanI G'GyrC_C

Cuts at:      0     926    2235 
   Size:        926    1309
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1633    2235 
   Size:       1633     602
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     218    2235 
   Size:        218    2017
 
BfrBI ATG'CAT

Cuts at:      0    1458    2235 
   Size:       1458     777
 
BglII A'GATC_T

Cuts at:      0    1879    2235 
   Size:       1879     356
 
BlpI GC'TnA_GC

Cuts at:      0      81    2235 
   Size:         81    2154
 
Bme1580I G_kGCm'C

Cuts at:      0    1143    2235 
   Size:       1143    1092
 
BmgBI CAC'GTC

Cuts at:      0    1858    2235 
   Size:       1858     377
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     911    1248    2235 
   Size:        911     337     987

  Fragments arranged by size: 

                 987     911     337
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1664    1821    2235 
   Size:       1664     157     414

  Fragments arranged by size: 

                1664     414     157
 
Bpu10I CC'TnA_GC

Cuts at:      0    1758    2235 
   Size:       1758     477
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1839    2235 
   Size:       1839     396
 
BsaAI yAC'GTr

Cuts at:      0     977    2235 
   Size:        977    1258
 
BsaBI GATnn'nnATC

Cuts at:      0     545    2235 
   Size:        545    1690
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1030    2235 
   Size:       1030    1205
 
BsiHKAI G_wGCw'C

Cuts at:      0    1143    2235 
   Size:       1143    1092
 
BsmI GAATG_Cn'

Cuts at:      0     454     880    1521    1781    2235 
   Size:        454     426     641     260     454

  Fragments arranged by size: 

                 641     454     454     426     260
 
Bsp1286I G_dGCh'C

Cuts at:      0    1143    2235 
   Size:       1143    1092
 
BsrDI GCAATG_nn'

Cuts at:      0    1460    2235 
   Size:       1460     775
 
BsrGI T'GTAC_A

Cuts at:      0     804    2235 
   Size:        804    1431
 
BstEII G'GTnAC_C

Cuts at:      0     615    2235 
   Size:        615    1620
 
BstYI r'GATC_y

Cuts at:      0     301     546    1879    2235 
   Size:        301     245    1333     356

  Fragments arranged by size: 

                1333     356     301     245
 
BtsI GCAGTG_nn'

Cuts at:      0     520    2235 
   Size:        520    1715
 
DraI TTT'AAA

Cuts at:      0    1329    2235 
   Size:       1329     906
 
DraIII CAC_nnn'GTG

Cuts at:      0    1307    2235 
   Size:       1307     928
 
EaeI y'GGCC_r

Cuts at:      0     841    2235 
   Size:        841    1394
 
EarI CTCTTCn'nnn_

Cuts at:      0     152     158     443    1967    1994    2235 
   Size:        152       6     285    1524      27     241

  Fragments arranged by size: 

                1524     285     241     152      27       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     642     890    1489    1664    1742    1821    1868 
   Size:        557      85     248     599     175      78      79      47

Cuts at:   1868    1886    2235 
   Size:         18     349

  Fragments arranged by size: 

                 599     557     349     248     175      85      79      78
                  47      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1937    2235 
   Size:       1937     298
 
EcoO109I rG'GnC_Cy

Cuts at:      0     900    2235 
   Size:        900    1335
 
EcoRI G'AATT_C

Cuts at:      0    2208    2235 
   Size:       2208      27
 
EcoRV GAT'ATC

Cuts at:      0    2169    2235 
   Size:       2169      66
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1366    1398    2214    2235 
   Size:       1366      32     816      21

  Fragments arranged by size: 

                1366     816      32      21
 
HincII GTy'rAC

Cuts at:      0     665    2235 
   Size:        665    1570
 
HindIII A'AGCT_T

Cuts at:      0    1209    2235 
   Size:       1209    1026
 
HpaI GTT'AAC

Cuts at:      0     665    2235 
   Size:        665    1570
 
KpnI G_GTAC'C

Cuts at:      0     930    2235 
   Size:        930    1305
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     258    1185    2235 
   Size:        258     927    1050

  Fragments arranged by size: 

                1050     927     258
 
MscI TGG'CCA

Cuts at:      0     843    2235 
   Size:        843    1392
 
MslI CAynn'nnrTG

Cuts at:      0    1600    2235 
   Size:       1600     635
 
MspA1I CmG'CkG

Cuts at:      0    1684    2119    2235 
   Size:       1684     435     116

  Fragments arranged by size: 

                1684     435     116
 
NsiI A_TGCA'T

Cuts at:      0    1460    2235 
   Size:       1460     775
 
NspI r_CATG'y

Cuts at:      0     370    1462    1599    2235 
   Size:        370    1092     137     636

  Fragments arranged by size: 

                1092     636     370     137
 
PciI A'CATG_T

Cuts at:      0     366    2235 
   Size:        366    1869
 
PpuMI rG'GwC_Cy

Cuts at:      0     900    2235 
   Size:        900    1335
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     961     993    2235 
   Size:        961      32    1242

  Fragments arranged by size: 

                1242     961      32
 
PstI C_TGCA'G

Cuts at:      0     176     527    2235 
   Size:        176     351    1708

  Fragments arranged by size: 

                1708     351     176
 
PvuII CAG'CTG

Cuts at:      0    1684    2235 
   Size:       1684     551
 
SanDI GG'GwC_CC

Cuts at:      0     900    2235 
   Size:        900    1335
 
SexAI A'CCwGG_T

Cuts at:      0     209    2235 
   Size:        209    2026
 
SfcI C'TryA_G

Cuts at:      0     172     523    1934    2235 
   Size:        172     351    1411     301

  Fragments arranged by size: 

                1411     351     301     172
 
SpeI A'CTAG_T

Cuts at:      0    1615    2235 
   Size:       1615     620
 
StyI C'CwwG_G

Cuts at:      0      92     904    2235 
   Size:         92     812    1331

  Fragments arranged by size: 

                1331     812      92
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1158    1284    1299    2235 
   Size:       1158     126      15     936

  Fragments arranged by size: 

                1158     936     126      15
 
TatI w'GTAC_w

Cuts at:      0     804    1109    1125    2089    2152    2235 
   Size:        804     305      16     964      63      83

  Fragments arranged by size: 

                 964     804     305      83      63      16
 
XmnI GAAnn'nnTTC

Cuts at:      0    1295    1781    2108    2235 
   Size:       1295     486     327     127

  Fragments arranged by size: 

                1295     486     327     127
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AlfI    AlwNI    ApaLI     ApoI     BanI
     BbsI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BsrDI    BsrGI   BstEII    BstYI     BtsI     DraI   DraIII
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    Hin4I
   HincII  HindIII     HpaI     KpnI     MmeI     MscI     MslI   MspA1I
     NsiI     NspI     PciI    PpuMI     PsrI     PstI    PvuII    SanDI
    SexAI     SfcI     SpeI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI     BclI     BglI
     BmtI     BplI    BpuEI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DrdI     EagI     EciI  EcoICRI     FalI
     FseI     FspI    FspAI    HaeII     KasI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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