Restriction map for pF1KB5080
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsmI        BspDI       
BspEI       BspLU11I    BspMI       BssHII      BssSI       
BstAPI      BstEII      BstPI       BstUI       Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       FseI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MspA1I      MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
StuI        SwaI        Tsp45I      Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AgeI           50  ACCGGT               A^CCGGT
AlwI         1168  GATCC                GGATC(4/5)
AviII         194  TGCGCA               TGC^GCA
BanII        1920  GAGCTC               G[GA]GC[CT]^C
BbsI         1628  GAAGAC               GAAGAC(2/6)
BcnI          621  CCGGG                CC^[GC]GG
BpuAI        1628  GAAGAC               GAAGAC(2/6)
BsaI         1540  GGTCTC               GGTCTC(1/5)
BsaWI          50  ACCGGT               [AT]^CCGG[AT]
Bsp1407I      216  TGTACA               T^GTACA
BspHI         605  TCATGA               T^CATGA
BsrBI         299  CCGCTC               CCGCTC(-3/-3)
BsrFI          50  ACCGGT               [GA]^CCGG[CT]
BsrGI         216  TGTACA               T^GTACA
Bst1107I     1644  GTATAC               GTA^TAC
BstBI        1144  TTCGAA               TT^CGAA
BstYI        1167  AGATCC               [GA]^GATC[CT]
BstZ17I      1644  GTATAC               GTA^TAC
Cfr10I         50  ACCGGT               [GA]^CCGG[CT]
DrdI         1888  GACATTCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          792  CCATGG               C^C[GA][CT]GG
EarI         1580  CTCTTC               CTCTTC(1/4)
EcoNI        1189  CCTGTGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       259  ATGCAT               ATGCA^T
FspI          194  TGCGCA               TGC^GCA
HaeII         870  AGCGCC               [GA]GCGC^[CT]
Ksp632I      1580  CTCTTC               CTCTTC(1/4)
MflI         1167  AGATCC               [GA]^GATC[CT]
NciI          621  CCGGG                CC^[GC]GG
NcoI          792  CCATGG               C^CATGG
NsiI          259  ATGCAT               ATGCA^T
NspV         1144  TTCGAA               TT^CGAA
PaeR7I       1223  CTCGAG               C^TCGAG
PinAI          50  ACCGGT               A^CCGGT
PpuMI         152  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          605  TCATGA               T^CATGA
SacI         1920  GAGCTC               GAGCT^C
SbfI          182  CCTGCAGG             CCTGCA^GG
SfuI         1144  TTCGAA               TT^CGAA
SphI         1724  GCATGC               GCATG^C
Sse8387I      182  CCTGCAGG             CCTGCA^GG
SspBI         216  TGTACA               T^GTACA
SspI         1375  AATATT               AAT^ATT
XcmI          497  CCAAATCCATAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1223  CTCGAG               C^TCGAG
XhoII        1167  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          748  GTAGAC               GT^[AC][GT]AC
             1644  GTATAC              
Alw44I       1207  GTGCAC               G^TGCAC
             1450  GTGCAC              
ApaLI        1207  GTGCAC               G^TGCAC
             1450  GTGCAC              
Asp700I       708  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2085  GAAAGTGTTC          
AvaI           66  CCCGAG               C^[CT]CG[GA]G
             1223  CTCGAG              
AvaII         153  GGTCC                G^G[AT]CC
              552  GGACC               
BalI          347  TGGCCA               TGG^CCA
             1422  TGGCCA              
BsmBI          61  CGTCTC               CGTCTC(1/5)
             1268  GAGACG              
BsmFI         955  GGGAC                GGGAC(10/14)
             1897  GTCCC               
BsoBI          66  CCCGAG               C^[CT]CG[GA]G
             1223  CTCGAG              
BsrDI         159  CATTGC               GCAATG(2/0)
              797  GCAATG              
CfoI          195  GCGC                 GCG^C
              871  GCGC                
EaeI          347  TGGCCA               [CT]^GGCC[GA]
             1422  TGGCCA              
FauI          298  CCCGC                CCCGC(4/6)
             2008  GCGGG               
HapII          51  CCGG                 C^CGG
              621  CCGG                
HhaI          195  GCGC                 GCG^C
              871  GCGC                
HinP1I        195  GCGC                 G^CGC
              871  GCGC                
HpaII          51  CCGG                 C^CGG
              621  CCGG                
MaeII        1448  ACGT                 A^CGT
             1699  ACGT                
MaeIII       1036  GTTAC                ^GT[ACGT]AC
             1696  GTAAC               
MluNI         347  TGGCCA               TGG^CCA
             1422  TGGCCA              
MscI          347  TGGCCA               TGG^CCA
             1422  TGGCCA              
MspI           51  CCGG                 C^CGG
              621  CCGG                
PflMI         868  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2065  CCAAAGGATGG         
SmlI         1223  CTCGAG               C^T[CT][GA]AG
             1549  CTCAAG              
Van91I        868  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2065  CCAAAGGATGG         
XmnI          708  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2085  GAAAGTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        AspHI       BbvI        BclI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MboI        MboII       MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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