Restriction map for
pF1KB5080
(Circular) (Six-base) MAPSORT of: pF1KB5080.seq Check: 9179 from: 1 to: 5288
pF1KB5080 5288 bp
With 182 enzymes: SgfI *
May 21, 2013 16:22 ..
AccI GT'mk_AC
Cuts at: 857 1753 2326 857
Size: 896 573 3819
Fragments arranged by size:
3819 896 573
Acc65I G'GTAC_C
Cuts at: 2304 2304
Size: 5288
AclI AA'CG_TT
Cuts at: 5128 5128
Size: 5288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1016 1675 3113 3545 4292 4560 700
Size: 316 659 1438 432 747 268 1428
Fragments arranged by size:
1438 1428 747 659 432 316 268
AflIII A'CryG_T
Cuts at: 2640 3744 4721 2640
Size: 1104 977 3207
Fragments arranged by size:
3207 1104 977
AgeI A'CCGG_T
Cuts at: 158 3676 158
Size: 3518 1770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 316 1262 316
Size: 946 4342
AlwNI CAG_nnn'CTG
Cuts at: 678 982 1066 1165 2593 4160 678
Size: 304 84 99 1428 1567 1806
Fragments arranged by size:
1806 1567 1428 304 99 84
ApaLI G'TGCA_C
Cuts at: 1315 1558 4058 1315
Size: 243 2500 2545
Fragments arranged by size:
2545 2500 243
ApoI r'AATT_y
Cuts at: 360 517 775 2292 4641 4897 360
Size: 157 258 1517 2349 256 751
Fragments arranged by size:
2349 1517 751 258 256 157
AseI AT'TA_AT
Cuts at: 20 20
Size: 5288
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
AvaI C'yCGr_G
Cuts at: 174 1331 2308 174
Size: 1157 977 3154
Fragments arranged by size:
3154 1157 977
BamHI G'GATC_C
Cuts at: 2313 2313
Size: 5288
BanI G'GyrC_C
Cuts at: 2304 2718 2966 3001 2304
Size: 414 248 35 4591
Fragments arranged by size:
4591 414 248 35
BanII G_rGCy'C
Cuts at: 2032 2302 3332 2032
Size: 270 1030 3988
Fragments arranged by size:
3988 1030 270
BbeI G_GCGC'C
Cuts at: 2722 2970 2722
Size: 248 5040
BbsI GAAGACnn'nnnn_
Cuts at: 1743 1743
Size: 5288
BciVI GTATCCnnnnn_n'
Cuts at: 3186 3947 5288 3186
Size: 761 1341 3186
Fragments arranged by size:
3186 1341 761
BclI T'GATC_A
Cuts at: 894 1154 1701 2808 894
Size: 260 547 1107 3374
Fragments arranged by size:
3374 1107 547 260
BfrBI ATG'CAT
Cuts at: 369 369
Size: 5288
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5288
BglII A'GATC_T
Cuts at: 2803 4840 2803
Size: 2037 3251
BlpI GC'TnA_GC
Cuts at: 2400 2400
Size: 5288
Bme1580I G_kGCm'C
Cuts at: 1319 1562 2913 3006 4062 1319
Size: 243 1351 93 1056 2545
Fragments arranged by size:
2545 1351 1056 243 93
BmrI ACTGGGnnnn_n'
Cuts at: 328 2500 2581 2916 5060 328
Size: 2172 81 335 2144 556
Fragments arranged by size:
2172 2144 556 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 3655 2143
Size: 1512 3776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 1382 2120 647
Size: 735 738 3815
Fragments arranged by size:
3815 738 735
Bpu10I CC'TnA_GC
Cuts at: 2055 2216 2055
Size: 161 5127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2464 3343 3835 4133 1642
Size: 822 879 492 298 2797
Fragments arranged by size:
2797 879 822 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1654 4992 1654
Size: 3338 1950
BsaAI yAC'GTr
Cuts at: 2641 2641
Size: 5288
BsaBI GATnn'nnATC
Cuts at: 6 2802 4839 6
Size: 2796 2037 455
Fragments arranged by size:
2796 2037 455
BsaHI Gr'CG_yC
Cuts at: 2719 2967 3669 5165 2719
Size: 248 702 1496 2842
Fragments arranged by size:
2842 1496 702 248
BsaWI w'CCGG_w
Cuts at: 158 2477 2998 3676 3950 4097 4508 158
Size: 2319 521 678 274 147 411 938
Fragments arranged by size:
2319 938 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 620 650 1367 1397 2093 2123 620
Size: 30 717 30 696 30 3785
Fragments arranged by size:
3785 717 696 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 293 554 752 982 1319 2266 293
Size: 261 198 230 337 947 3315
Fragments arranged by size:
3315 947 337 261 230 198
BseYI C'CCAG_C
Cuts at: 416 975 1011 1549 2713 3471 4048 4544 416
Size: 559 36 538 1164 758 577 496 1160
Fragments arranged by size:
1164 1160 758 577 559 538 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 961 1875 455
Size: 506 914 3868
Fragments arranged by size:
3868 914 506
BsiEI CG_ry'CG
Cuts at: 104 2830 2876 4084 104
Size: 2726 46 1208 1308
Fragments arranged by size:
2726 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 601 1319 1562 2032 2217 2302 3080 3270 4062
Size: 718 243 470 185 85 778 190 792
Cuts at: 4062 601
Size: 1827
Fragments arranged by size:
1827 792 778 718 470 243 190 185
85
BsmBI CGTCTCn'nnnn_
Cuts at: 175 1370 175
Size: 1195 4093
Bsp1286I G_dGCh'C
Cuts at: 601 1319 1562 2032 2217 2302 2913 3006 3080
Size: 718 243 470 185 85 611 93 74
Cuts at: 3080 3270 3332 4062 601
Size: 190 62 730 1827
Fragments arranged by size:
1827 730 718 611 470 243 190 185
93 85 74 62
BspEI T'CCGG_A
Cuts at: 4508 4508
Size: 5288
BspHI T'CATG_A
Cuts at: 713 4464 713
Size: 3751 1537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2338 2552 2854 3235 2338
Size: 214 302 381 4391
Fragments arranged by size:
4391 381 302 214
BsrBI CCG'CTC
Cuts at: 50 409 3580 3634 5118 5283 50
Size: 359 3171 54 1484 165 55
Fragments arranged by size:
3171 1484 359 165 55 54
BsrDI GCAATG_nn'
Cuts at: 266 912 3200 266
Size: 646 2288 2354
Fragments arranged by size:
2354 2288 646
BsrFI r'CCGG_y
Cuts at: 158 3286 3467 3676 158
Size: 3128 181 209 1770
Fragments arranged by size:
3128 1770 209 181
BsrGI T'GTAC_A
Cuts at: 324 324
Size: 5288
BssSI C'ACGA_G
Cuts at: 3559 3917 3559
Size: 358 4930
BstAPI GCAn_nnn'nTGC
Cuts at: 2665 2665
Size: 5288
BstBI TT'CG_AA
Cuts at: 1253 3649 1253
Size: 2396 2892
BstXI CCAn_nnnn'nTGG
Cuts at: 318 889 1864 2151 318
Size: 571 975 287 3455
Fragments arranged by size:
3455 975 571 287
BstYI r'GATC_y
Cuts at: 1 1275 2313 2780 2803 3138 3384 4396 4482
Size: 1274 1038 467 23 335 246 1012 86
Cuts at: 4482 4494 4840 1
Size: 12 346 449
Fragments arranged by size:
1274 1038 1012 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1754 1754
Size: 5288
BtgI C'CryG_G
Cuts at: 107 900 107
Size: 793 4495
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3238 3419 3582 3238
Size: 181 163 4944
Fragments arranged by size:
4944 181 163
BtsI GCAGTG_nn'
Cuts at: 1734 2531 1734
Size: 797 4491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1335 1370 1488 1523 1335
Size: 35 118 35 5100
Fragments arranged by size:
5100 118 35 35
DraI TTT'AAA
Cuts at: 2287 2287
Size: 5288
DrdI GACnn_nn'nnGTC
Cuts at: 2002 2994 3852 2002
Size: 992 858 3438
Fragments arranged by size:
3438 992 858
EaeI y'GGCC_r
Cuts at: 455 1530 2873 3047 3438 3465 4810 455
Size: 1075 1343 174 391 27 1345 933
Fragments arranged by size:
1345 1343 1075 933 391 174 27
EagI C'GGCC_G
Cuts at: 2873 2873
Size: 5288
EarI CTCTTCn'nnn_
Cuts at: 1694 3311 3521 1694
Size: 1617 210 3461
Fragments arranged by size:
3461 1617 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 163 3496 3806 3952 5098 163
Size: 3333 310 146 1146 353
Fragments arranged by size:
3333 1146 353 310 146
EcoICRI GAG'CTC
Cuts at: 2030 2300 2030
Size: 270 5018
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 700 1016 1382 1675 2120 3113 3545 4292
Size: 53 316 366 293 445 993 432 747
Cuts at: 4292 4560 647
Size: 268 1375
Fragments arranged by size:
1375 993 747 445 432 366 316 293
268 53
EcoNI CCTnn'n_nnAGG
Cuts at: 1301 1301
Size: 5288
EcoO109I rG'GnC_Cy
Cuts at: 261 559 972 2427 261
Size: 298 413 1455 3122
Fragments arranged by size:
3122 1455 413 298
EcoRI G'AATT_C
Cuts at: 2292 2292
Size: 5288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 5288
FspI TGC'GCA
Cuts at: 304 3069 304
Size: 2765 2523
FspAI rTGC'GCAy
Cuts at: 304 304
Size: 5288
HaeII r_GCGC'y
Cuts at: 982 2722 2970 3992 4971 982
Size: 1740 248 1022 979 1299
Fragments arranged by size:
1740 1299 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 649 681 936 968 1693 1725 1730 1762 2245
Size: 32 255 32 725 32 5 32 483
Cuts at: 2245 2277 3130 3160 3162 3192 649
Size: 32 853 30 2 30 2745
Fragments arranged by size:
2745 853 725 483 255 32 32 32
32 32 30 30 5 2
HincII GTy'rAC
Cuts at: 2327 2327
Size: 5288
KasI G'GCGC_C
Cuts at: 2718 2966 2718
Size: 248 5040
KpnI G_GTAC'C
Cuts at: 2308 2308
Size: 5288
MluI A'CGCG_T
Cuts at: 4721 4721
Size: 5288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1035 1470 1585 3959 4143 1035
Size: 435 115 2374 184 2180
Fragments arranged by size:
2374 2180 435 184 115
MscI TGG'CCA
Cuts at: 457 1532 3049 457
Size: 1075 1517 2696
Fragments arranged by size:
2696 1517 1075
MslI CAynn'nnrTG
Cuts at: 204 451 905 1111 1297 2134 2149 3277 3404
Size: 247 454 206 186 837 15 1128 127
Cuts at: 3404 204
Size: 2088
Fragments arranged by size:
2088 1128 837 454 247 206 186 127
15
MspA1I CmG'CkG
Cuts at: 2399 2713 3073 4086 4331 2399
Size: 314 360 1013 245 3356
Fragments arranged by size:
3356 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3469 3469
Size: 5288
NarI GG'CG_CC
Cuts at: 2719 2967 2719
Size: 248 5040
NcoI C'CATG_G
Cuts at: 107 900 107
Size: 793 4495
NgoMIV G'CCGG_C
Cuts at: 3467 3467
Size: 5288
NsiI A_TGCA'T
Cuts at: 371 371
Size: 5288
NspI r_CATG'y
Cuts at: 373 874 1836 2341 2841 3748 373
Size: 501 962 505 500 907 1913
Fragments arranged by size:
1913 962 907 505 501 500
PasI CC'CwG_GG
Cuts at: 1644 1644
Size: 5288
PciI A'CATG_T
Cuts at: 3744 3744
Size: 5288
PflMI CCAn_nnn'nTGG
Cuts at: 982 2179 982
Size: 1197 4091
PfoI T'CCnGG_A
Cuts at: 486 1946 486
Size: 1460 3828
PmeI GTTT'AAAC
Cuts at: 2287 2287
Size: 5288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 5256
PpuMI rG'GwC_Cy
Cuts at: 261 261
Size: 5288
PspXI vC'TCGA_Gb
Cuts at: 1331 1331
Size: 5288
PstI C_TGCA'G
Cuts at: 295 1026 1241 1617 2335 3020 295
Size: 731 215 376 718 685 2563
Fragments arranged by size:
2563 731 718 685 376 215
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5288
PvuII CAG'CTG
Cuts at: 2713 3073 2713
Size: 360 4928
RsrII CG'GwC_CG
Cuts at: 3483 3483
Size: 5288
SacI G_AGCT'C
Cuts at: 2032 2302 2032
Size: 270 5018
SalI G'TCGA_C
Cuts at: 2325 2325
Size: 5288
SapI GCTCTTCn'nnn_
Cuts at: 3311 3521 3311
Size: 210 5078
SbfI CC_TGCA'GG
Cuts at: 295 2335 295
Size: 2040 3248
ScaI AGT'ACT
Cuts at: 4805 4805
Size: 5288
SfcI C'TryA_G
Cuts at: 33 291 933 1022 1237 1613 2202 2331 3016
Size: 258 642 89 215 376 589 129 685
Cuts at: 3016 4009 4200 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 642 589 376 258 215
191 129 89
SfoI GGC'GCC
Cuts at: 2720 2968 2720
Size: 248 5040
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
SmaI CCC'GGG
Cuts at: 2310 2310
Size: 5288
SmlI C'TyrA_G
Cuts at: 1331 1657 2443 3358 3850 4112 1331
Size: 326 786 915 492 262 2507
Fragments arranged by size:
2507 915 786 492 326 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5288
SphI G_CATG'C
Cuts at: 1836 2341 2841 1836
Size: 505 500 4283
Fragments arranged by size:
4283 505 500
SspI AAT'ATT
Cuts at: 1485 1485
Size: 5288
StyI C'CwwG_G
Cuts at: 107 257 900 1863 1973 2422 2776 107
Size: 150 643 963 110 449 354 2619
Fragments arranged by size:
2619 963 643 449 354 150 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 192 310 1550 1690 2871 3004 3463 3672 192
Size: 118 1240 140 1181 133 459 209 1808
Fragments arranged by size:
1808 1240 1181 459 209 140 133 118
TatI w'GTAC_w
Cuts at: 227 324 914 1478 4803 227
Size: 97 590 564 3325 712
Fragments arranged by size:
3325 712 590 564 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 464 496 2093 2125 4805 4837 464
Size: 32 1597 32 2680 32 915
Fragments arranged by size:
2680 1597 915 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3085 3085
Size: 5288
XbaI T'CTAG_A
Cuts at: 2319 2319
Size: 5288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 612 2783 612
Size: 2171 3117
XhoI C'TCGA_G
Cuts at: 1331 1331
Size: 5288
XmaI C'CCGG_G
Cuts at: 2308 2308
Size: 5288
XmnI GAAnn'nnTTC
Cuts at: 820 2197 820
Size: 1377 3911
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI BsiWI
BsmI BssHII BstEII Bsu36I ClaI DraIII EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PmlI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI