Restriction map for pF1KB5080
 (Linear) (Six-base) MAPSORT of: KIBB5080.seq  Check: 2605  from: 1  to: 2175



KIBB5080.orf

 With 182 enzymes: SgfI * 

                        May 21, 2013 16:22  ..

AccI GT'mk_AC

Cuts at:      0     749    1645    2175 
   Size:        749     896     530

  Fragments arranged by size: 

                 896     749     530
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     908    1567    2175 
   Size:        592     316     659     608

  Fragments arranged by size: 

                 659     608     592     316
 
AgeI A'CCGG_T

Cuts at:      0      50    2175 
   Size:         50    2125
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     208    1154    2175 
   Size:        208     946    1021

  Fragments arranged by size: 

                1021     946     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0     570     874     958    1057    2175 
   Size:        570     304      84      99    1118

  Fragments arranged by size: 

                1118     570     304      99      84
 
ApaLI G'TGCA_C

Cuts at:      0    1207    1450    2175 
   Size:       1207     243     725

  Fragments arranged by size: 

                1207     725     243
 
ApoI r'AATT_y

Cuts at:      0     252     409     667    2175 
   Size:        252     157     258    1508

  Fragments arranged by size: 

                1508     258     252     157
 
AvaI C'yCGr_G

Cuts at:      0      66    1223    2175 
   Size:         66    1157     952

  Fragments arranged by size: 

                1157     952      66
 
BanII G_rGCy'C

Cuts at:      0    1924    2175 
   Size:       1924     251
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1635    2175 
   Size:       1635     540
 
BclI T'GATC_A

Cuts at:      0     786    1046    1593    2175 
   Size:        786     260     547     582

  Fragments arranged by size: 

                 786     582     547     260
 
BfrBI ATG'CAT

Cuts at:      0     261    2175 
   Size:        261    1914
 
Bme1580I G_kGCm'C

Cuts at:      0    1211    1454    2175 
   Size:       1211     243     721

  Fragments arranged by size: 

                1211     721     243
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     220    2175 
   Size:        220    1955
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2035    2175 
   Size:       2035     140
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539    1274    2012    2175 
   Size:        539     735     738     163

  Fragments arranged by size: 

                 738     735     539     163
 
Bpu10I CC'TnA_GC

Cuts at:      0    1947    2108    2175 
   Size:       1947     161      67

  Fragments arranged by size: 

                1947     161      67
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1534    2175 
   Size:       1534     641
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1546    2175 
   Size:       1546     629
 
BsaWI w'CCGG_w

Cuts at:      0      50    2175 
   Size:         50    2125
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     512     542    1259    1289    1985    2015    2175 
   Size:        512      30     717      30     696      30     160

  Fragments arranged by size: 

                 717     696     512     160      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     185     446     644     874    1211    2158    2175 
   Size:        185     261     198     230     337     947      17

  Fragments arranged by size: 

                 947     337     261     230     198     185      17
 
BseYI C'CCAG_C

Cuts at:      0     308     867     903    1441    2175 
   Size:        308     559      36     538     734

  Fragments arranged by size: 

                 734     559     538     308      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     853    1767    2175 
   Size:        347     506     914     408

  Fragments arranged by size: 

                 914     506     408     347
 
BsiHKAI G_wGCw'C

Cuts at:      0     493    1211    1454    1924    2109    2175 
   Size:        493     718     243     470     185      66

  Fragments arranged by size: 

                 718     493     470     243     185      66
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      67    1262    2175 
   Size:         67    1195     913

  Fragments arranged by size: 

                1195     913      67
 
Bsp1286I G_dGCh'C

Cuts at:      0     493    1211    1454    1924    2109    2175 
   Size:        493     718     243     470     185      66

  Fragments arranged by size: 

                 718     493     470     243     185      66
 
BspHI T'CATG_A

Cuts at:      0     605    2175 
   Size:        605    1570
 
BsrBI CCG'CTC

Cuts at:      0     301    2175 
   Size:        301    1874
 
BsrDI GCAATG_nn'

Cuts at:      0     158     804    2175 
   Size:        158     646    1371

  Fragments arranged by size: 

                1371     646     158
 
BsrFI r'CCGG_y

Cuts at:      0      50    2175 
   Size:         50    2125
 
BsrGI T'GTAC_A

Cuts at:      0     216    2175 
   Size:        216    1959
 
BstBI TT'CG_AA

Cuts at:      0    1145    2175 
   Size:       1145    1030
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     781    1756    2043    2175 
   Size:        210     571     975     287     132

  Fragments arranged by size: 

                 975     571     287     210     132
 
BstYI r'GATC_y

Cuts at:      0    1167    2175 
   Size:       1167    1008
 
BstZ17I GTA'TAC

Cuts at:      0    1646    2175 
   Size:       1646     529
 
BtgI C'CryG_G

Cuts at:      0     792    2175 
   Size:        792    1383
 
BtsI GCAGTG_nn'

Cuts at:      0    1626    2175 
   Size:       1626     549
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1227    1262    1380    1415    2175 
   Size:       1227      35     118      35     760

  Fragments arranged by size: 

                1227     760     118      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1894    2175 
   Size:       1894     281
 
EaeI y'GGCC_r

Cuts at:      0     347    1422    2175 
   Size:        347    1075     753

  Fragments arranged by size: 

                1075     753     347
 
EarI CTCTTCn'nnn_

Cuts at:      0    1586    2175 
   Size:       1586     589
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      55    2175 
   Size:         55    2120
 
EcoICRI GAG'CTC

Cuts at:      0    1922    2175 
   Size:       1922     253
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539     592     908    1274    1567    2012    2175 
   Size:        539      53     316     366     293     445     163

  Fragments arranged by size: 

                 539     445     366     316     293     163      53
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1193    2175 
   Size:       1193     982
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153     451     864    2175 
   Size:        153     298     413    1311

  Fragments arranged by size: 

                1311     413     298     153
 
FspI TGC'GCA

Cuts at:      0     196    2175 
   Size:        196    1979
 
FspAI rTGC'GCAy

Cuts at:      0     196    2175 
   Size:        196    1979
 
HaeII r_GCGC'y

Cuts at:      0     874    2175 
   Size:        874    1301
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     541     573     828     860    1585    1617    1622    1654 
   Size:        541      32     255      32     725      32       5      32

Cuts at:   1654    2137    2169    2175 
   Size:        483      32       6

  Fragments arranged by size: 

                 725     541     483     255      32      32      32      32
                  32       6       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     927    1362    1477    2175 
   Size:        927     435     115     698

  Fragments arranged by size: 

                 927     698     435     115
 
MscI TGG'CCA

Cuts at:      0     349    1424    2175 
   Size:        349    1075     751

  Fragments arranged by size: 

                1075     751     349
 
MslI CAynn'nnrTG

Cuts at:      0      96     343     797    1003    1189    2026    2041    2175 
   Size:         96     247     454     206     186     837      15     134

  Fragments arranged by size: 

                 837     454     247     206     186     134      96      15
            
 
NcoI C'CATG_G

Cuts at:      0     792    2175 
   Size:        792    1383
 
NsiI A_TGCA'T

Cuts at:      0     263    2175 
   Size:        263    1912
 
NspI r_CATG'y

Cuts at:      0     265     766    1728    2175 
   Size:        265     501     962     447

  Fragments arranged by size: 

                 962     501     447     265
 
PasI CC'CwG_GG

Cuts at:      0    1536    2175 
   Size:       1536     639
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     874    2071    2175 
   Size:        874    1197     104

  Fragments arranged by size: 

                1197     874     104
 
PfoI T'CCnGG_A

Cuts at:      0     378    1838    2175 
   Size:        378    1460     337

  Fragments arranged by size: 

                1460     378     337
 
PpuMI rG'GwC_Cy

Cuts at:      0     153    2175 
   Size:        153    2022
 
PspXI vC'TCGA_Gb

Cuts at:      0    1223    2175 
   Size:       1223     952
 
PstI C_TGCA'G

Cuts at:      0     187     918    1133    1509    2175 
   Size:        187     731     215     376     666

  Fragments arranged by size: 

                 731     666     376     215     187
 
SacI G_AGCT'C

Cuts at:      0    1924    2175 
   Size:       1924     251
 
SbfI CC_TGCA'GG

Cuts at:      0     187    2175 
   Size:        187    1988
 
SfcI C'TryA_G

Cuts at:      0     183     825     914    1129    1505    2094    2175 
   Size:        183     642      89     215     376     589      81

  Fragments arranged by size: 

                 642     589     376     215     183      89      81
 
SmlI C'TyrA_G

Cuts at:      0    1223    1549    2175 
   Size:       1223     326     626

  Fragments arranged by size: 

                1223     626     326
 
SphI G_CATG'C

Cuts at:      0    1728    2175 
   Size:       1728     447
 
SspI AAT'ATT

Cuts at:      0    1377    2175 
   Size:       1377     798
 
StyI C'CwwG_G

Cuts at:      0     149     792    1755    1865    2175 
   Size:        149     643     963     110     310

  Fragments arranged by size: 

                 963     643     310     149     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      84     202    1442    1582    2175 
   Size:         84     118    1240     140     593

  Fragments arranged by size: 

                1240     593     140     118      84
 
TatI w'GTAC_w

Cuts at:      0     119     216     806    1370    2175 
   Size:        119      97     590     564     805

  Fragments arranged by size: 

                 805     590     564     119      97
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     356     388    1985    2017    2175 
   Size:        356      32    1597      32     158

  Fragments arranged by size: 

                1597     356     158      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     504    2175 
   Size:        504    1671
 
XhoI C'TCGA_G

Cuts at:      0    1223    2175 
   Size:       1223     952
 
XmnI GAAnn'nnTTC

Cuts at:      0     712    2089    2175 
   Size:        712    1377      86

  Fragments arranged by size: 

                1377     712      86
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AlfI    AlwNI    ApaLI     ApoI     AvaI
    BanII     BbsI     BclI    BfrBI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
    BstBI    BstXI    BstYI  BstZ17I     BtgI     BtsI    CspCI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI
    FspAI    HaeII    Hin4I     MmeI     MscI     MslI     NcoI     NsiI
     NspI     PasI    PflMI     PfoI    PpuMI    PspXI     PstI     SacI
     SbfI     SfcI     SmlI     SphI     SspI     StyI    TaqII     TatI
     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BglI    BglII
     BlpI    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI
     BsmI    BspEI    BspMI   BssHII    BssSI   BstAPI   BstEII   Bsu36I
    BtgZI     ClaI     DraI   DraIII     EagI    EcoRI    EcoRV     FalI
     FseI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XmaI     ZraI
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