Restriction map for pF1KB8685
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BamHI       BbrPI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraI        Eco47III    EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MluI        MroI        MseI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SapI        SbfI        
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI         1603  GTCGAC               GT^[AC][GT]AC
AcsI         1081  GAATTC               [GA]^AATT[CT]
AhdI          703  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          697  CCTGAGG              CC^T[ACGT]AGG
ApaI         1445  GGGCCC               GGGCC^C
ApoI         1081  GAATTC               [GA]^AATT[CT]
Asp700I      1368  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         703  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1201  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1264  GGCGCC               GGCGC^C
BsaI            5  GAGACC               GGTCTC(1/5)
BsiEI        1466  CGGCCG               CG[GA][CT]^CG
BsrBI        1257  CCGCTC               CCGCTC(-3/-3)
BsrFI         940  GCCGGC               [GA]^CCGG[CT]
BstXI         905  CCAACATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        697  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        940  GCCGGC               [GA]^CCGG[CT]
DraIII        738  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1390  GACACGCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1466  CGGCCG               C^GGCCG
Eam1105I      703  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1466  CGGCCG               C^GGCCG
Eco52I       1466  CGGCCG               C^GGCCG
Eco81I        697  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1081  GAATTC               G^AATTC
KasI         1264  GGCGCC               G^GCGCC
MaeII        1328  ACGT                 A^CGT
MfeI          633  CAATTG               C^AATTG
MunI          633  CAATTG               C^AATTG
NaeI          940  GCCGGC               GCC^GGC
NarI         1264  GGCGCC               GG^CGCC
NgoMIV        940  GCCGGC               G^CCGGC
PflFI        1201  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         994  CAGCTG               CAG^CTG
SalI         1603  GTCGAC               G^TCGAC
ScaI         1853  AGTACT               AGT^ACT
SfoI         1264  GGCGCC               GGC^GCC
TfiI         1049  GAATC                G^A[AT]TC
Tth111I      1201  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1368  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII        1316  CGCG                 CG^CG
             1549  CGCG                
BalI          983  TGGCCA               TGG^CCA
             1613  TGGCCA              
BbsI         1683  GAAGAC               GAAGAC(2/6)
              220  GTCTTC              
BglI         1256  GCCGCTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1484  GCCCGCTGGGC         
BpuAI        1683  GAAGAC               GAAGAC(2/6)
              220  GTCTTC              
BsgI         1659  CTGCAC               GTGCAG(16/14)
              595  GTGCAG              
BstUI        1316  CGCG                 CG^CG
             1549  CGCG                
BstYI         680  AGATCC               [GA]^GATC[CT]
             1040  AGATCC              
EarI         1717  GAAGAG               CTCTTC(1/4)
             2050  GAAGAG              
HincII        427  GTCAAC               GT[CT]^[GA]AC
             1603  GTCGAC              
HindII        427  GTCAAC               GT[CT]^[GA]AC
             1603  GTCGAC              
Ksp632I      1717  GAAGAG               CTCTTC(1/4)
             2050  GAAGAG              
MflI          680  AGATCC               [GA]^GATC[CT]
             1040  AGATCC              
MluNI         983  TGGCCA               TGG^CCA
             1613  TGGCCA              
MscI          983  TGGCCA               TGG^CCA
             1613  TGGCCA              
MvnI         1316  CGCG                 CG^CG
             1549  CGCG                
NcoI         1309  CCATGG               C^CATGG
             1610  CCATGG              
PpuMI         701  AGGACCC              [GA]G^G[AT]CC[CT]
             1700  AGGACCT             
SacI          337  GAGCTC               GAGCT^C
              954  GAGCTC              
SexAI         659  ACCTGGT              A^CC[AT]GGT
             1064  ACCTGGT             
SmaI         1419  CCCGGG               CCC^GGG
             1888  CCCGGG              
StuI           65  AGGCCT               AGG^CCT
              608  AGGCCT              
Tsp509I       634  AATT                 ^AATT
             1082  AATT                
XcmI          800  CCACCTACAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              986  CCATGAAACAGCTGG     
XhoII         680  AGATCC               [GA]^GATC[CT]
             1040  AGATCC              
XmaCI        1419  CCCGGG               C^CCGGG
             1888  CCCGGG              
XmaI         1419  CCCGGG               C^CCGGG
             1888  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvCI       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      
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