Restriction map for pF1KB8685
 (Linear) (Six-base) MAPSORT of: KIBB8685.seq  Check: 9755  from: 1  to: 2151



KIBB8685.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AccI GT'mk_AC

Cuts at:      0    1604    2151 
   Size:       1604     547
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     494     502     878    2151 
   Size:        494       8     376    1273

  Fragments arranged by size: 

                1273     494     376       8
 
AhdI GACnn_n'nnGTC

Cuts at:      0     708    2151 
   Size:        708    1443
 
AleI CACnn'nnGTG

Cuts at:      0     554    1666    2151 
   Size:        554    1112     485

  Fragments arranged by size: 

                1112     554     485
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1606    2151 
   Size:       1606     545
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105     399    1844    2064    2151 
   Size:        105     294    1445     220      87

  Fragments arranged by size: 

                1445     294     220     105      87
 
ApaI G_GGCC'C

Cuts at:      0    1449    2151 
   Size:       1449     702
 
ApoI r'AATT_y

Cuts at:      0    1081    2151 
   Size:       1081    1070
 
AvaI C'yCGr_G

Cuts at:      0     118     574     787    1419    1888    1960    2151 
   Size:        118     456     213     632     469      72     191

  Fragments arranged by size: 

                 632     469     456     213     191     118      72
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1987    2020    2151 
   Size:       1987      33     131

  Fragments arranged by size: 

                1987     131      33
 
BanI G'GyrC_C

Cuts at:      0     478     564    1264    1812    2151 
   Size:        478      86     700     548     339

  Fragments arranged by size: 

                 700     548     478     339      86
 
BanII G_rGCy'C

Cuts at:      0     341     422     575     958    1449    2151 
   Size:        341      81     153     383     491     702

  Fragments arranged by size: 

                 702     491     383     341     153      81
 
BbeI G_GCGC'C

Cuts at:      0    1268    2151 
   Size:       1268     883
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     213    1690    2151 
   Size:        213    1477     461

  Fragments arranged by size: 

                1477     461     213
 
BbvCI CC'TCA_GC

Cuts at:      0     230     299     643    2151 
   Size:        230      69     344    1508

  Fragments arranged by size: 

                1508     344     230      69
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1262    1490    2151 
   Size:       1262     228     661

  Fragments arranged by size: 

                1262     661     228
 
Bme1580I G_kGCm'C

Cuts at:      0     483     561     905    1449    2151 
   Size:        483      78     344     544     702

  Fragments arranged by size: 

                 702     544     483     344      78
 
BmgBI CAC'GTC

Cuts at:      0    1329    2151 
   Size:       1329     822
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     418    1629    2003    2151 
   Size:        418    1211     374     148

  Fragments arranged by size: 

                1211     418     374     148
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      77    1275    2151 
   Size:         77    1198     876

  Fragments arranged by size: 

                1198     876      77
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     352     604     739    1319    1615    2151 
   Size:        125     227     252     135     580     296     536

  Fragments arranged by size: 

                 580     536     296     252     227     135     125
 
Bpu10I CC'TnA_GC

Cuts at:      0      23      98     230     299     569     643     952    1260 
   Size:         23      75     132      69     270      74     309     308

Cuts at:   1260    2151 
   Size:        891

  Fragments arranged by size: 

                 891     309     308     270     132      75      74      69
                  23
 
BsaHI Gr'CG_yC

Cuts at:      0     146     944    1265    2151 
   Size:        146     798     321     886

  Fragments arranged by size: 

                 886     798     321     146
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136     892     895    1111    1114    1123    1126    1129 
   Size:        136     756       3     216       3       9       3       3

Cuts at:   1129    1138    1180    1183    1192    1201    1264    1525    1528 
   Size:          9      42       3       9       9      63     261       3

Cuts at:   1528    1540    1543    1576    1705    1708    1711    1726    1756 
   Size:         12       3      33     129       3       3      15      30

Cuts at:   1756    2068    2116    2119    2122    2125    2134    2137    2140 
   Size:        312      48       3       3       3       9       3       3

Cuts at:   2140    2143    2151 
   Size:          3       8

  Fragments arranged by size: 

                 756     312     261     216     136     129      63      48
                  42      33      30      15      12       9       9       9
                   9       9       8       3       3       3       3       3
                   3       3       3       3       3       3       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0      35     149     243     482     537    1488    1543    1940 
   Size:         35     114      94     239      55     951      55     397

Cuts at:   1940    2151 
   Size:        211

  Fragments arranged by size: 

                 951     397     239     211     114      94      55      55
                  35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616    1644    2151 
   Size:        616    1028     507

  Fragments arranged by size: 

                1028     616     507
 
BsiEI CG_ry'CG

Cuts at:      0    1469    2151 
   Size:       1469     682
 
BsiHKAI G_wGCw'C

Cuts at:      0     341     659     958    2151 
   Size:        341     318     299    1193

  Fragments arranged by size: 

                1193     341     318     299
 
Bsp1286I G_dGCh'C

Cuts at:      0     341     422     483     561     575     659     905     958 
   Size:        341      81      61      78      14      84     246      53

Cuts at:    958    1449    2151 
   Size:        491     702

  Fragments arranged by size: 

                 702     491     341     246      84      81      78      61
                  53      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      17     318     623    1448    1467    2151 
   Size:         17     301     305     825      19     684

  Fragments arranged by size: 

                 825     684     305     301      19      17
 
BsrBI CCG'CTC

Cuts at:      0    1259    2151 
   Size:       1259     892
 
BsrFI r'CCGG_y

Cuts at:      0     940    2151 
   Size:        940    1211
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     635    1644    1844    2151 
   Size:        635    1009     200     307

  Fragments arranged by size: 

                1009     635     307     200
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     912    2151 
   Size:        912    1239
 
BstYI r'GATC_y

Cuts at:      0     680    1040    2151 
   Size:        680     360    1111

  Fragments arranged by size: 

                1111     680     360
 
Bsu36I CC'TnA_GG

Cuts at:      0     698    2151 
   Size:        698    1453
 
BtgI C'CryG_G

Cuts at:      0    1196    1309    1610    2151 
   Size:       1196     113     301     541

  Fragments arranged by size: 

                1196     541     301     113
 
BtsI GCAGTG_nn'

Cuts at:      0     748    1768    2151 
   Size:        748    1020     383

  Fragments arranged by size: 

                1020     748     383
 
DraIII CAC_nnn'GTG

Cuts at:      0     743    2151 
   Size:        743    1408
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1396    2151 
   Size:       1396     755
 
EaeI y'GGCC_r

Cuts at:      0     590     983    1070    1466    1613    1862    2151 
   Size:        590     393      87     396     147     249     289

  Fragments arranged by size: 

                 590     396     393     289     249     147      87
 
EagI C'GGCC_G

Cuts at:      0    1466    2151 
   Size:       1466     685
 
EarI CTCTTCn'nnn_

Cuts at:      0    1712    2045    2151 
   Size:       1712     333     106

  Fragments arranged by size: 

                1712     333     106
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     829    2151 
   Size:        829    1322
 
EcoICRI GAG'CTC

Cuts at:      0     339     956    2151 
   Size:        339     617    1195

  Fragments arranged by size: 

                1195     617     339
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     352     494     502     604     739     878    1319 
   Size:        125     227     142       8     102     135     139     441

Cuts at:   1319    1615    2151 
   Size:        296     536

  Fragments arranged by size: 

                 536     441     296     227     142     139     135     125
                 102       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      72    1648    1708    2151 
   Size:         72    1576      60     443

  Fragments arranged by size: 

                1576     443      72      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     102     702    1701    1892    2151 
   Size:        102     600     999     191     259

  Fragments arranged by size: 

                 999     600     259     191     102
 
EcoRI G'AATT_C

Cuts at:      0    1081    2151 
   Size:       1081    1070
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1389    2151 
   Size:       1389     762
 
HaeII r_GCGC'y

Cuts at:      0      30     832    1268    2151 
   Size:         30     802     436     883

  Fragments arranged by size: 

                 883     802     436      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     454     486     694     726    2151 
   Size:        247      32     175      32     208      32    1425

  Fragments arranged by size: 

                1425     247     208     175      32      32      32
 
HincII GTy'rAC

Cuts at:      0     429    1605    2151 
   Size:        429    1176     546

  Fragments arranged by size: 

                1176     546     429
 
KasI G'GCGC_C

Cuts at:      0    1264    2151 
   Size:       1264     887
 
MfeI C'AATT_G

Cuts at:      0     633    2151 
   Size:        633    1518
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1901    2151 
   Size:       1901     250
 
MscI TGG'CCA

Cuts at:      0     985    1615    2151 
   Size:        985     630     536

  Fragments arranged by size: 

                 985     630     536
 
MslI CAynn'nnrTG

Cuts at:      0     554    1588    1666    2029    2151 
   Size:        554    1034      78     363     122

  Fragments arranged by size: 

                1034     554     363     122      78
 
MspA1I CmG'CkG

Cuts at:      0     112     363     693     996    1488    1658    2151 
   Size:        112     251     330     303     492     170     493

  Fragments arranged by size: 

                 493     492     330     303     251     170     112
 
NaeI GCC'GGC

Cuts at:      0     942    2151 
   Size:        942    1209
 
NarI GG'CG_CC

Cuts at:      0    1265    2151 
   Size:       1265     886
 
NcoI C'CATG_G

Cuts at:      0    1309    1610    2151 
   Size:       1309     301     541

  Fragments arranged by size: 

                1309     541     301
 
NgoMIV G'CCGG_C

Cuts at:      0     940    2151 
   Size:        940    1211
 
PasI CC'CwG_GG

Cuts at:      0    2088    2151 
   Size:       2088      63
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     399     743    1883    2151 
   Size:        399     344    1140     268

  Fragments arranged by size: 

                1140     399     344     268
 
PfoI T'CCnGG_A

Cuts at:      0     413     716    1085    2151 
   Size:        413     303     369    1066

  Fragments arranged by size: 

                1066     413     369     303
 
PpuMI rG'GwC_Cy

Cuts at:      0     702    1701    2151 
   Size:        702     999     450

  Fragments arranged by size: 

                 999     702     450
 
PspOMI G'GGCC_C

Cuts at:      0    1445    2151 
   Size:       1445     706
 
PstI C_TGCA'G

Cuts at:      0     362     623    1840    2151 
   Size:        362     261    1217     311

  Fragments arranged by size: 

                1217     362     311     261
 
PvuII CAG'CTG

Cuts at:      0     996    2151 
   Size:        996    1155
 
SacI G_AGCT'C

Cuts at:      0     341     958    2151 
   Size:        341     617    1193

  Fragments arranged by size: 

                1193     617     341
 
SalI G'TCGA_C

Cuts at:      0    1603    2151 
   Size:       1603     548
 
ScaI AGT'ACT

Cuts at:      0    1855    2151 
   Size:       1855     296
 
SexAI A'CCwGG_T

Cuts at:      0     659    1064    2151 
   Size:        659     405    1087

  Fragments arranged by size: 

                1087     659     405
 
SfcI C'TryA_G

Cuts at:      0     358     552     619    1836    2151 
   Size:        358     194      67    1217     315

  Fragments arranged by size: 

                1217     358     315     194      67
 
SfoI GGC'GCC

Cuts at:      0    1266    2151 
   Size:       1266     885
 
SmaI CCC'GGG

Cuts at:      0    1421    1890    2151 
   Size:       1421     469     261

  Fragments arranged by size: 

                1421     469     261
 
StuI AGG'CCT

Cuts at:      0      67     610    2151 
   Size:         67     543    1541

  Fragments arranged by size: 

                1541     543      67
 
StyI C'CwwG_G

Cuts at:      0     434     605    1244    1309    1610    2151 
   Size:        434     171     639      65     301     541

  Fragments arranged by size: 

                 639     541     434     301     171      65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     235    1611    1656    2151 
   Size:        235    1376      45     495

  Fragments arranged by size: 

                1376     495     235      45
 
TatI w'GTAC_w

Cuts at:      0    1280    1853    1952    2151 
   Size:       1280     573      99     199

  Fragments arranged by size: 

                1280     573     199      99
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     264     296    1625    1657    2151 
   Size:        264      32    1329      32     494

  Fragments arranged by size: 

                1329     494     264      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1204    2151 
   Size:       1204     947
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     807     993    2151 
   Size:        807     186    1158

  Fragments arranged by size: 

                1158     807     186
 
XmaI C'CCGG_G

Cuts at:      0    1419    1888    2151 
   Size:       1419     469     263

  Fragments arranged by size: 

                1419     469     263
 
XmnI GAAnn'nnTTC

Cuts at:      0    1372    2151 
   Size:       1372     779
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaHI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     KasI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SalI
     ScaI    SexAI     SfcI     SfoI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MluI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SapI
     SbfI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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