Restriction map for pF1KB3033
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AhdI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BamHI       BbrPI       BbsI        BcgI        
BciVI       BclI        BfaI        BfrI        BlnI        
BpuAI       BsaBI       BsaI        BseAI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DrdI        Eam1105I    EarI        
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HindIII     HpaI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tsp509I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AflIII       1676  ACGCGT               A^C[GA][CT]GT
AgeI          908  ACCGGT               A^CCGGT
Alw44I        967  GTGCAC               G^TGCAC
AlwNI         898  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1315  CCTCAGG              CC^T[ACGT]AGG
ApaLI         967  GTGCAC               G^TGCAC
AspI          811  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI           12  GGCGCC               GGCGC^C
BbvCI        1488  GCTGAGG              CCTCAGC(-5/-2)
BlpI         1013  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1013  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1063  TACGTG               [CT]AC^GT[GA]
BsiEI         977  CGGCCG               CG[GA][CT]^CG
BsmI         1494  GAATGC               GAATGC(1/-1)
BspHI        1328  TCATGA               T^CATGA
BstXI         434  CCAAGACCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1315  CCTCAGG              CC^T[ACGT]AGG
CelII        1013  GCTCAGC              GC^T[ACGT]AGC
DraIII        961  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          977  CGGCCG               C^GGCCG
EclXI         977  CGGCCG               C^GGCCG
Eco52I        977  CGGCCG               C^GGCCG
Eco81I       1315  CCTCAGG              CC^T[ACGT]AGG
FseI           57  GGCCGGCC             GGCCGG^CC
HaeII          12  GGCGCC               [GA]GCGC^[CT]
HincII       1408  GTTGAC               GT[CT]^[GA]AC
HindII       1408  GTTGAC               GT[CT]^[GA]AC
HinfI        1267  GACTC                G^A[ACGT]TC
KasI           12  GGCGCC               G^GCGCC
MluI         1676  ACGCGT               A^CGCGT
NarI           12  GGCGCC               GG^CGCC
PflFI         811  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         908  ACCGGT               A^CCGGT
PleI         1267  GACTC                GAGTC(4/5)
RcaI         1328  TCATGA               T^CATGA
SfoI           12  GGCGCC               GGC^GCC
StuI          155  AGGCCT               AGG^CCT
Tth111I       811  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          733  GTCTAC               GT^[AC][GT]AC
             1369  GTCTAC              
AvaI          567  CCCGAG               C^[CT]CG[GA]G
             1568  CCCGAG              
BanI           12  GGCGCC               G^G[CT][GA]CC
              663  GGCACC              
BglI          313  GCCATTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              712  GCCATGCTGGC         
BglII         377  AGATCT               A^GATCT
             1340  AGATCT              
BsaWI         509  TCCGGT               [AT]^CCGG[AT]
              908  ACCGGT              
BsgI          232  GTGCAG               GTGCAG(16/14)
             1468  GTGCAG              
BsmBI        1057  CGTCTC               CGTCTC(1/5)
             1393  GAGACG              
BsoBI         567  CCCGAG               C^[CT]CG[GA]G
             1568  CCCGAG              
BspMI         888  GCAGGT               ACCTGC(4/8)
             1632  GCAGGT              
EcoNI         147  CCTGCAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              744  CCTTCGGGAGG         
EcoT14I       258  CCATGG               C^C[AT][AT]GG
              440  CCATGG              
MaeIII        704  GTGAC                ^GT[ACGT]AC
              817  GTCAC               
MseI          254  TTAA                 T^TAA
              629  TTAA                
NcoI          258  CCATGG               C^CATGG
              440  CCATGG              
NspI          398  ACATGC               [GA]CATG^[CT]
             1682  ACATGC              
PflMI         787  CCAGCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              897  CCAGAACCTGG         
PvuII         788  CAGCTG               CAG^CTG
             1402  CAGCTG              
StyI          258  CCATGG               C^C[AT][AT]GG
              440  CCATGG              
Tru9I         254  TTAA                 T^TAA
              629  TTAA                
Tsp45I        704  GTGAC                ^GT[GC]AC
              817  GTCAC               
Van91I        787  CCAGCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              897  CCAGAACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        AlwI        ApaI        AspHI       AvaII       
BalI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        MaeII       MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
TspRI       TthHB8I     XcmI        XhoII       
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