Restriction map for pF1KE0113
##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII          66  CGCG                 CG^CG
AluI          230  AGCT                 AG^CT
AlwI           84  GATCC                GGATC(4/5)
BcnI          249  CCCGG                CC^[GC]GG
BsaI          163  GAGACC               GGTCTC(1/5)
BseRI         139  GAGGAG               GAGGAG(10/8)
BsgI          187  GTGCAG               GTGCAG(16/14)
BsmAI         163  GAGAC                GTCTC(1/5)
BsmFI         204  GGGAC                GGGAC(10/14)
BspMI         104  ACCTGC               ACCTGC(4/8)
BsrDI          57  CATTGC               GCAATG(2/0)
BsrI          126  CCAGT                ACTGG(1/-1)
BstUI          66  CGCG                 CG^CG
BstXI          26  CCACGGGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          117  GCGC                 GCG^C
DpnII          84  GATC                 ^GATC
DraII          76  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          197  CGGCCA               [CT]^GGCC[GA]
EcoO109I       76  GGGGCCT              [GA]G^G[ACGT]CC[CT]
HapII         250  CCGG                 C^CGG
HhaI          117  GCGC                 GCG^C
HinP1I        117  GCGC                 G^CGC
HinfI         237  GACTC                G^A[ACGT]TC
HpaII         250  CCGG                 C^CGG
MaeIII         37  GTCAC                ^GT[ACGT]AC
MboI           84  GATC                 ^GATC
MslI           27  CACGGGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          250  CCGG                 C^CGG
MvnI           66  CGCG                 CG^CG
NciI          249  CCCGG                CC^[GC]GG
NdeII          84  GATC                 ^GATC
PleI          237  GACTC                GAGTC(4/5)
PstI          112  CTGCAG               CTGCA^G
Sau3AI         84  GATC                 ^GATC
SfcI          112  CTGCAG               C^T[GA][CT]AG
SfiI           69  GGCCCTGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I         37  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           67  GCGG                 CCGC(-3/-1)
              108  GCGG                
AvaII          12  GGACC                G^G[AT]CC
              205  GGACC               
BglI           61  GCCATCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               70  GCCCTGGGGGC         
BstF5I         51  CATCC                GGATG(2/0)
              156  GGATG               
BstNI          72  CCTGG                CC^[AT]GG
               87  CCTGG               
DsaI           26  CCACGG               C^C[GA][CT]GG
              258  CCACGG              
EcoRII         72  CCTGG                ^CC[AT]GG
               87  CCTGG               
EcoT14I       167  CCAAGG               C^C[AT][AT]GG
              200  CCAAGG              
FokI           51  CATCC                GGATG(9/13)
              156  GGATG               
MvaI           72  CCTGG                CC^[AT]GG
               87  CCTGG               
SfaNI         119  GCATC                GCATC(5/9)
              146  GCATC               
StyI          167  CCAAGG               C^C[AT][AT]GG
              200  CCAAGG              
XcmI           23  CCACCACGGGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
               62  CCATCGCGGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

BbvI        BsaJI       BsiYI       BslI        BssKI       
Cfr13I      Fnu4HI      HaeIII      ItaI        MnlI        
MwoI        NlaIV       Sau96I      ScrFI       TseI        
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