Restriction map for pF1KB3608
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BmyI        BsaAI       BsaBI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrGI       BsrI        BssHII      Bst1107I    BstAPI      
BstBI       BstF5I      BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         203  CGCG                 CG^CG
AcsI          580  AAATTC               [GA]^AATT[CT]
AcyI          230  GGCGCC               G[GA]^CG[CT]C
AlwI           18  GATCC                GGATC(4/5)
Aor51HI       290  AGCGCT               AGC^GCT
ApoI          580  AAATTC               [GA]^AATT[CT]
AvaII          87  GGACC                G^G[AT]CC
BanI          230  GGCGCC               G^G[CT][GA]CC
BbeI          230  GGCGCC               GGCGC^C
BbsI          424  GAAGAC               GAAGAC(2/6)
BglII         221  AGATCT               A^GATCT
BlpI          172  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      172  GCTGAGC              GC^T[ACGT]AGC
BpuAI         424  GAAGAC               GAAGAC(2/6)
BsaHI         230  GGCGCC               G[GA]^CG[CT]C
BsaWI         575  ACCGGA               [AT]^CCGG[AT]
BsrDI         168  CATTGC               GCAATG(2/0)
BssSI         137  CTCGTG               CACGAG(-5/-1)
BstEII        528  GGTGACC              G^GT[ACGT]ACC
BstPI         528  GGTGACC              G^GT[ACGT]ACC
BstUI         203  CGCG                 CG^CG
BstXI         254  CCAACTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         221  AGATCT               [GA]^GATC[CT]
CelII         172  GCTGAGC              GC^T[ACGT]AGC
DsaI          410  CCACGG               C^C[GA][CT]GG
Eco47III      290  AGCGCT               AGC^GCT
EcoO65I       528  GGTGACC              G^GT[ACGT]ACC
FauI          101  CCCGC                CCCGC(4/6)
Hin1I         230  GGCGCC               G[GA]^CG[CT]C
HinfI         548  GAGTC                G^A[ACGT]TC
KasI          230  GGCGCC               G^GCGCC
MaeII         320  ACGT                 A^CGT
MflI          221  AGATCT               [GA]^GATC[CT]
MseI          401  TTAA                 T^TAA
MvnI          203  CGCG                 CG^CG
NarI          230  GGCGCC               GG^CGCC
NspI          436  GCATGC               [GA]CATG^[CT]
PleI          548  GAGTC                GAGTC(4/5)
SfoI          230  GGCGCC               GGC^GCC
SmaI          483  CCCGGG               CCC^GGG
SphI          436  GCATGC               GCATG^C
Tru9I         401  TTAA                 T^TAA
Tsp509I       581  AATT                 ^AATT
XcmI          410  CCACGGTGACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         221  AGATCT               [GA]^GATC[CT]
XmaCI         483  CCCGGG               C^CCGGG
XmaI          483  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaI          483  CCCGGG               C^[CT]CG[GA]G
              524  CTCGGG              
BpmI          113  CTGGAG               CTGGAG(16/14)
              124  CTGGAG              
BsaI           56  GAGACC               GGTCTC(1/5)
              616  GAGACC              
BsoBI         483  CCCGGG               C^[CT]CG[GA]G
              524  CTCGGG              
BsrBI          21  CCGCTC               CCGCTC(-3/-3)
              102  CCGCTC              
BsrFI         177  GCCGGC               [GA]^CCGG[CT]
              554  GCCGGC              
Cfr10I        177  GCCGGC               [GA]^CCGG[CT]
              554  GCCGGC              
DdeI            4  CTGAG                C^T[ACGT]AG
              173  CTGAG               
EaeI          179  CGGCCA               [CT]^GGCC[GA]
              268  TGGCCG              
EcoT14I       563  CCAAGG               C^C[AT][AT]GG
              621  CCTTGG              
HaeII         230  GGCGCC               [GA]GCGC^[CT]
              290  AGCGCT              
HgaI          201  GACGC                GACGC(5/10)
              417  GACGC               
MspA1I        346  CAGCTG               C[AC]G^C[GT]G
              458  CAGCTG              
NaeI          177  GCCGGC               GCC^GGC
              554  GCCGGC              
NgoMIV        177  GCCGGC               G^CCGGC
              554  GCCGGC              
PvuII         346  CAGCTG               CAG^CTG
              458  CAGCTG              
StyI          563  CCAAGG               C^C[AT][AT]GG
              621  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsaJI       
BsgI        BsiYI       BslI        BsmAI       BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DpnII       
EcoRII      Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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