Restriction map for pF1KB3684
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        Alw44I      AlwI        AlwNI       
Aor51HI     ApaLI       ApoI        AscI        Asp700I     
Asp718I     AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BssHII      
BssSI       BstBI       BstEII      BstPI       BstXI       
CelII       ClaI        CpoI        DraI        DrdI        
DsaI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
FbaI        FseI        FspI        HincII      HindII      
HpaI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SfiI        SfuI        SmaI        SnaBI       SpeI        
Sse8387I    SspI        StyI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         1002  GTATAC               GT^[AC][GT]AC
AflIII        831  ACGTGT               A^C[GA][CT]GT
AhdI          412  GACTGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          107  CCTCAGG              CC^T[ACGT]AGG
AseI          265  ATTAAT               AT^TAAT
AsnI          265  ATTAAT               AT^TAAT
AspEI         412  GACTGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          344  GGCGCC               GGCGC^C
BbsI         1225  GAAGAC               GAAGAC(2/6)
BcnI         1770  CCGGG                CC^[GC]GG
BglII        1508  AGATCT               A^GATCT
BpmI           80  CTGGAG               CTGGAG(16/14)
BpuAI        1225  GAAGAC               GAAGAC(2/6)
BsaBI         271  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1662  GAGACG               CGTCTC(1/5)
BsrFI        1673  ACCGGC               [GA]^CCGG[CT]
Bst1107I     1002  GTATAC               GTA^TAC
BstAPI       1776  GCAGTGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1508  AGATCT               [GA]^GATC[CT]
BstZ17I      1002  GTATAC               GTA^TAC
Bsu36I        107  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1673  ACCGGC               [GA]^CCGG[CT]
Eam1105I      412  GACTGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        107  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      1645  ATGCAT               ATGCA^T
HindIII      1529  AAGCTT               A^AGCTT
KasI          344  GGCGCC               G^GCGCC
MamI          271  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1508  AGATCT               [GA]^GATC[CT]
MspA1I       1523  CAGCTG               C[AC]G^C[GT]G
NarI          344  GGCGCC               GG^CGCC
NciI         1770  CCGGG                CC^[GC]GG
NsiI         1645  ATGCAT               ATGCA^T
PflMI         858  CCACAAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         247  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         265  ATTAAT               AT^TAAT
PvuII        1523  CAGCTG               CAG^CTG
SapI          331  GAAGAGC              GCTCTTC(1/4)
SexAI        1139  ACCTGGT              A^CC[AT]GGT
SfoI          344  GGCGCC               GGC^GCC
SgrAI        1672  CACCGGCG             C[GA]^CCGG[CT]G
SphI         1781  GCATGC               GCATG^C
Van91I        858  CCACAAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1508  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         610  CGCG                 CG^CG
             1710  CGCG                
AspHI         210  GAGCTC               G[AT]GC[AT]^C
             1201  GAGCTC              
BglI          517  GCCTGTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1187  GCCACGACGGC         
BsaJI         340  CCTCGG               C^C[ACGT][ACGT]GG
             1690  CCTGGG              
BsiHKAI       210  GAGCTC               G[AT]GC[AT]^C
             1201  GAGCTC              
BsmFI        1424  GGGAC                GGGAC(10/14)
              855  GTCCC               
Bsp1407I      351  TGTACA               T^GTACA
             1181  TGTACA              
BsrGI         351  TGTACA               T^GTACA
             1181  TGTACA              
BstUI         610  CGCG                 CG^CG
             1710  CGCG                
DraIII        807  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1138  CACCTGGTG           
HaeII         344  GGCGCC               [GA]GCGC^[CT]
             1676  GGCGCT              
HapII        1674  CCGG                 C^CGG
             1770  CCGG                
HpaII        1674  CCGG                 C^CGG
             1770  CCGG                
MaeII         831  ACGT                 A^CGT
              892  ACGT                
MslI          114  CATTGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1252  CACAGTGATG          
MspI         1674  CCGG                 C^CGG
             1770  CCGG                
MvnI          610  CGCG                 CG^CG
             1710  CGCG                
NspI         1628  ACATGC               [GA]CATG^[CT]
             1781  GCATGC              
PstI          553  CTGCAG               CTGCA^G
              902  CTGCAG              
SacI          210  GAGCTC               GAGCT^C
             1201  GAGCTC              
ScaI          681  AGTACT               AGT^ACT
             1113  AGTACT              
SfaNI         913  GATGC                GCATC(5/9)
             1647  GCATC               
SfcI          553  CTGCAG               C^T[GA][CT]AG
              902  CTGCAG              
SspBI         351  TGTACA               T^GTACA
             1181  TGTACA              
StuI          125  AGGCCT               AGG^CCT
              515  AGGCCT              
TfiI          748  GATTC                G^A[AT]TC
             1017  GAATC               
Tsp509I      1089  AATT                 ^AATT
             1321  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        ApaI        
BalI        BanI        BanII       BbvI        BfaI        
BmyI        BsaHI       BseRI       BsgI        BsiYI       
BslI        BsmAI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HgaI        HhaI        
Hin1I       HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SmlI        
TaqI        Tru9I       TseI        Tsp45I      TspRI       
TthHB8I     
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