Restriction map for pF1KE0096
##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspI        AviII       AvrII       BbrPI       
BcgI        BfrI        BglI        BglII       BlnI        
BsaAI       BsiEI       BsiWI       BsmBI       BspDI       
BsrFI       BssHII      BstXI       Cfr10I      ClaI        
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      FseI        FspI        HpaI        KspI        
MfeI        MluI        MunI        NaeI        NgoMIV      
NotI        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        ScaI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SwaI        Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccII         180  CGCG                 CG^CG
AccIII         27  TCCGGA               T^CCGGA
AcyI           21  GGCGCC               G[GA]^CG[CT]C
AhdI         4504  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        4504  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         3302  TGGCCA               TGG^CCA
BanII        3272  GAGCTC               G[GA]GC[CT]^C
BbeI           21  GGCGCC               GGCGC^C
BbvCI        4381  CCTCAGC              CCTCAGC(-5/-2)
BclI         4227  TGATCA               T^GATCA
BcnI          620  CCGGG                CC^[GC]GG
BsaBI        4981  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          21  GGCGCC               G[GA]^CG[CT]C
BsaI          145  GGTCTC               GGTCTC(1/5)
BsaWI          27  TCCGGA               [AT]^CCGG[AT]
BseAI          27  TCCGGA               T^CCGGA
BspEI          27  TCCGGA               T^CCGGA
BssSI        1670  CTCGTG               CACGAG(-5/-1)
Bst1107I     4289  GTATAC               GTA^TAC
BstBI        3435  TTCGAA               TT^CGAA
BstEII       3513  GGTAACC              G^GT[ACGT]ACC
BstPI        3513  GGTAACC              G^GT[ACGT]ACC
BstUI         180  CGCG                 CG^CG
BstZ17I      4289  GTATAC               GTA^TAC
DraII        2208  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          446  CCATGG               C^C[GA][CT]GG
EaeI         3302  TGGCCA               [CT]^GGCC[GA]
Eam1105I     4504  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2008  CCTGTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     2208  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      3513  GGTAACC              G^GT[ACGT]ACC
EcoT22I      3862  ATGCAT               ATGCA^T
FbaI         4227  TGATCA               T^GATCA
HaeII          21  GGCGCC               [GA]GCGC^[CT]
HgaI          426  GCGTC                GACGC(5/10)
Hin1I          21  GGCGCC               G[GA]^CG[CT]C
HincII       2453  GTCAAC               GT[CT]^[GA]AC
HindII       2453  GTCAAC               GT[CT]^[GA]AC
KasI           21  GGCGCC               G^GCGCC
MamI         4981  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        3302  TGGCCA               TGG^CCA
MroI           27  TCCGGA               T^CCGGA
MscI         3302  TGGCCA               TGG^CCA
MvnI          180  CGCG                 CG^CG
NarI           21  GGCGCC               GG^CGCC
NciI          620  CCGGG                CC^[GC]GG
NcoI          446  CCATGG               C^CATGG
NheI         5397  GCTAGC               G^CTAGC
NruI          179  TCGCGA               TCG^CGA
NsiI         3862  ATGCAT               ATGCA^T
NspV         3435  TTCGAA               TT^CGAA
PflMI        4840  CCATTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2208  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        3901  GACTCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         3272  GAGCTC               GAGCT^C
SapI         2857  GCTCTTC              GCTCTTC(1/4)
SbfI         1906  CCTGCAGG             CCTGCA^GG
SfoI           21  GGCGCC               GGC^GCC
SfuI         3435  TTCGAA               TT^CGAA
Sse8387I     1906  CCTGCAGG             CCTGCA^GG
StuI          990  AGGCCT               AGG^CCT
Van91I       4840  CCATTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        336  GGTACC               G^GTACC
             2334  GGTACC              
AflIII       1331  ACATGT               A^C[GA][CT]GT
             3768  ACATGT              
AocI         3283  CCTCAGG              CC^T[ACGT]AGG
             3407  CCTCAGG             
Asp700I       239  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              881  GAAATCTTTC          
Asp718I       336  GGTACC               G^GTACC
             2334  GGTACC              
AvaI         1255  CTCGAG               C^[CT]CG[GA]G
             1622  CTCGAG              
BamHI         643  GGATCC               G^GATCC
             4849  GGATCC              
BlpI         1018  GCTGAGC              GC^T[ACGT]AGC
             3349  GCTCAGC             
Bpu1102I     1018  GCTGAGC              GC^T[ACGT]AGC
             3349  GCTCAGC             
BsmI         3135  GAATGC               GAATGC(1/-1)
             4531  GAATGC              
BsoBI        1255  CTCGAG               C^[CT]CG[GA]G
             1622  CTCGAG              
BspHI         768  TCATGA               T^CATGA
             3774  TCATGA              
BspLU11I     1331  ACATGT               A^CATGT
             3768  ACATGT              
BsrBI          34  GAGCGG               CCGCTC(-3/-3)
             3609  GAGCGG              
Bsu36I       3283  CCTCAGG              CC^T[ACGT]AGG
             3407  CCTCAGG             
CelII        1018  GCTGAGC              GC^T[ACGT]AGC
             3349  GCTCAGC             
Eco81I       3283  CCTCAGG              CC^T[ACGT]AGG
             3407  CCTCAGG             
EcoT14I       446  CCATGG               C^C[AT][AT]GG
             3492  CCAAGG              
FauI         4767  CCCGC                CCCGC(4/6)
              852  GCGGG               
HapII          28  CCGG                 C^CGG
              620  CCGG                
HindIII      3226  AAGCTT               A^AGCTT
             5417  AAGCTT              
HpaII          28  CCGG                 C^CGG
              620  CCGG                
KpnI          336  GGTACC               GGTAC^C
             2334  GGTACC              
MspI           28  CCGG                 C^CGG
              620  CCGG                
NdeI         2398  CATATG               CA^TATG
             4748  CATATG              
PaeR7I       1255  CTCGAG               C^TCGAG
             1622  CTCGAG              
RcaI          768  TCATGA               T^CATGA
             3774  TCATGA              
SexAI        3216  ACCAGGT              A^CC[AT]GGT
             3509  ACCAGGT             
StyI          446  CCATGG               C^C[AT][AT]GG
             3492  CCAAGG              
XcmI         3492  CCAAGGGACTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5402  CCATTAGTAACATGG     
XhoI         1255  CTCGAG               C^TCGAG
             1622  CTCGAG              
XmnI          239  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              881  GAAATCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanI        BbsI        BbvI        BciVI       BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraIII      EarI        
EcoRI       EcoRII      EcoRV       Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com