Restriction map for pF1KE0096
 (Linear) (Six-base) MAPSORT of: KIEE0096.seq  Check: 4167  from: 1  to: 5505



KIEE0096.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1914    3558    5505 
   Size:       1914    1644    1947

  Fragments arranged by size: 

                1947    1914    1644
 
AccI GT'mk_AC

Cuts at:      0    3909    4290    4513    5505 
   Size:       3909     381     223     992

  Fragments arranged by size: 

                3909     992     381     223
 
Acc65I G'GTAC_C

Cuts at:      0     336    2334    5505 
   Size:        336    1998    3171

  Fragments arranged by size: 

                3171    1998     336
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844    2336    2579    5505 
   Size:        844    1492     243    2926

  Fragments arranged by size: 

                2926    1492     844     243
 
AflIII A'CryG_T

Cuts at:      0    1331    3768    5505 
   Size:       1331    2437    1737

  Fragments arranged by size: 

                2437    1737    1331
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4509    5505 
   Size:       4509     996
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1541    2105    4232    5505 
   Size:       1541     564    2127    1273

  Fragments arranged by size: 

                2127    1541    1273     564
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1707    1739    5505 
   Size:       1707      32    3766

  Fragments arranged by size: 

                3766    1707      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2101    3189    4963    5505 
   Size:       2101    1088    1774     542

  Fragments arranged by size: 

                2101    1774    1088     542
 
ApoI r'AATT_y

Cuts at:      0    1127    2048    2160    2271    2604    3932    4241    4368 
   Size:       1127     921     112     111     333    1328     309     127

Cuts at:   4368    4586    5505 
   Size:        218     919

  Fragments arranged by size: 

                1328    1127     921     919     333     309     218     127
                 112     111
 
AvaI C'yCGr_G

Cuts at:      0    1255    1622    5505 
   Size:       1255     367    3883

  Fragments arranged by size: 

                3883    1255     367
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2318    2351    5505 
   Size:       2318      33    3154

  Fragments arranged by size: 

                3154    2318      33
 
BamHI G'GATC_C

Cuts at:      0     643    4849    5505 
   Size:        643    4206     656

  Fragments arranged by size: 

                4206     656     643
 
BanI G'GyrC_C

Cuts at:      0      21     336    2334    5505 
   Size:         21     315    1998    3171

  Fragments arranged by size: 

                3171    1998     315      21
 
BanII G_rGCy'C

Cuts at:      0    3276    5505 
   Size:       3276    2229
 
BbeI G_GCGC'C

Cuts at:      0      25    5505 
   Size:         25    5480
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     688     928    5129    5505 
   Size:        688     240    4201     376

  Fragments arranged by size: 

                4201     688     376     240
 
BbvCI CC'TCA_GC

Cuts at:      0    4382    5505 
   Size:       4382    1123
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3837    4488    4554    5505 
   Size:       3837     651      66     951

  Fragments arranged by size: 

                3837     951     651      66
 
BclI T'GATC_A

Cuts at:      0    4227    5505 
   Size:       4227    1278
 
BfrBI ATG'CAT

Cuts at:      0    3864    5505 
   Size:       3864    1641
 
BlpI GC'TnA_GC

Cuts at:      0    1019    3350    5505 
   Size:       1019    2331    2155

  Fragments arranged by size: 

                2331    2155    1019
 
Bme1580I G_kGCm'C

Cuts at:      0    3431    5167    5505 
   Size:       3431    1736     338

  Fragments arranged by size: 

                3431    1736     338
 
BmgBI CAC'GTC

Cuts at:      0     567    5505 
   Size:        567    4938
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    5204    5505 
   Size:       5204     301
 
BmtI G_CTAG'C

Cuts at:      0    5401    5505 
   Size:       5401     104
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1520    2078    3329    3575    4883    5505 
   Size:       1520     558    1251     246    1308     622

  Fragments arranged by size: 

                1520    1308    1251     622     558     246
 
Bpu10I CC'TnA_GC

Cuts at:      0    4382    5505 
   Size:       4382    1123
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2649    3644    3888    5505 
   Size:       2649     995     244    1617

  Fragments arranged by size: 

                2649    1617     995     244
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151    5505 
   Size:        151    5354
 
BsaBI GATnn'nnATC

Cuts at:      0    4985    5505 
   Size:       4985     520
 
BsaHI Gr'CG_yC

Cuts at:      0      22    5505 
   Size:         22    5483
 
BsaWI w'CCGG_w

Cuts at:      0      27    5505 
   Size:         27    5478
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     200     230    2171    2201    3684    3714    3790    3820 
   Size:        200      30    1941      30    1483      30      76      30

Cuts at:   3820    5505 
   Size:       1685

  Fragments arranged by size: 

                1941    1685    1483     200      76      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     910    2219    3754    3833    5247    5505 
   Size:        910    1309    1535      79    1414     258

  Fragments arranged by size: 

                1535    1414    1309     910     258      79
 
BseYI C'CCAG_C

Cuts at:      0     825    1494    2931    3237    3477    5505 
   Size:        825     669    1437     306     240    2028

  Fragments arranged by size: 

                2028    1437     825     669     306     240
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     941    3352    4828    5201    5505 
   Size:        941    2411    1476     373     304

  Fragments arranged by size: 

                2411    1476     941     373     304
 
BsiHKAI G_wGCw'C

Cuts at:      0    3276    3958    5187    5505 
   Size:       3276     682    1229     318

  Fragments arranged by size: 

                3276    1229     682     318
 
BsmI GAATG_Cn'

Cuts at:      0    3141    4537    5505 
   Size:       3141    1396     968

  Fragments arranged by size: 

                3141    1396     968
 
Bsp1286I G_dGCh'C

Cuts at:      0    3276    3431    3958    5167    5187    5505 
   Size:       3276     155     527    1209      20     318

  Fragments arranged by size: 

                3276    1209     527     318     155      20
 
BspEI T'CCGG_A

Cuts at:      0      27    5505 
   Size:         27    5478
 
BspHI T'CATG_A

Cuts at:      0     768    3774    5505 
   Size:        768    3006    1731

  Fragments arranged by size: 

                3006    1731     768
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1914    3063    3310    3558    5505 
   Size:       1914    1149     247     248    1947

  Fragments arranged by size: 

                1947    1914    1149     248     247
 
BsrBI CCG'CTC

Cuts at:      0      36    3611    5505 
   Size:         36    3575    1894

  Fragments arranged by size: 

                3575    1894      36
 
BsrDI GCAATG_nn'

Cuts at:      0     909    1770    2281    2684    3404    3584    3843    5505 
   Size:        909     861     511     403     720     180     259    1662

  Fragments arranged by size: 

                1662     909     861     720     511     403     259     180
            
 
BsrGI T'GTAC_A

Cuts at:      0    2010    4752    4800    5505 
   Size:       2010    2742      48     705

  Fragments arranged by size: 

                2742    2010     705      48
 
BssSI C'ACGA_G

Cuts at:      0    1670    5505 
   Size:       1670    3835
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     570    1387    3670    5505 
   Size:        570     817    2283    1835

  Fragments arranged by size: 

                2283    1835     817     570
 
BstBI TT'CG_AA

Cuts at:      0    3436    5505 
   Size:       3436    2069
 
BstEII G'GTnAC_C

Cuts at:      0    3513    5505 
   Size:       3513    1992
 
BstYI r'GATC_y

Cuts at:      0     135     585     643    2345    4680    4849    4866    5505 
   Size:        135     450      58    1702    2335     169      17     639

  Fragments arranged by size: 

                2335    1702     639     450     169     135      58      17
            
 
BstZ17I GTA'TAC

Cuts at:      0    4291    5505 
   Size:       4291    1214
 
Bsu36I CC'TnA_GG

Cuts at:      0    3284    3408    5505 
   Size:       3284     124    2097

  Fragments arranged by size: 

                3284    2097     124
 
BtgI C'CryG_G

Cuts at:      0     446    5505 
   Size:        446    5059
 
BtsI GCAGTG_nn'

Cuts at:      0     875    5002    5505 
   Size:        875    4127     503

  Fragments arranged by size: 

                4127     875     503
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     497     532     642     677    5505 
   Size:        497      35     110      35    4828

  Fragments arranged by size: 

                4828     497     110      35      35
 
DraI TTT'AAA

Cuts at:      0     102     266    1109    3881    5505 
   Size:        102     164     843    2772    1624

  Fragments arranged by size: 

                2772    1624     843     164     102
 
DraIII CAC_nnn'GTG

Cuts at:      0     411    2331    3220    5505 
   Size:        411    1920     889    2285

  Fragments arranged by size: 

                2285    1920     889     411
 
EaeI y'GGCC_r

Cuts at:      0    3302    5505 
   Size:       3302    2203
 
EarI CTCTTCn'nnn_

Cuts at:      0     215    1735    2864    5505 
   Size:        215    1520    1129    2641

  Fragments arranged by size: 

                2641    1520    1129     215
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     188    5505 
   Size:        188    5317
 
EcoICRI GAG'CTC

Cuts at:      0    3274    5505 
   Size:       3274    2231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844    1520    2078    2336    2579    3329    3575    4883 
   Size:        844     676     558     258     243     750     246    1308

Cuts at:   4883    5505 
   Size:        622

  Fragments arranged by size: 

                1308     844     750     676     622     558     258     246
                 243
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2012    5505 
   Size:       2012    3493
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2209    5505 
   Size:       2209    3296
 
EcoRI G'AATT_C

Cuts at:      0    2048    2160    2271    4368    4586    5505 
   Size:       2048     112     111    2097     218     919

  Fragments arranged by size: 

                2097    2048     919     218     112     111
 
EcoRV GAT'ATC

Cuts at:      0    4044    4169    4200    5505 
   Size:       4044     125      31    1305

  Fragments arranged by size: 

                4044    1305     125      31
 
HaeII r_GCGC'y

Cuts at:      0      25    5505 
   Size:         25    5480
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1197    1229    1818    1850    3790    3822    4430    4453 
   Size:       1197      32     589      32    1940      32     608      23

Cuts at:   4453    4462    4485    4495    4527    5505 
   Size:          9      23      10      32     978

  Fragments arranged by size: 

                1940    1197     978     608     589      32      32      32
                  32      23      23      10       9
 
HincII GTy'rAC

Cuts at:      0    2455    5505 
   Size:       2455    3050
 
HindIII A'AGCT_T

Cuts at:      0    3226    5417    5505 
   Size:       3226    2191      88

  Fragments arranged by size: 

                3226    2191      88
 
KasI G'GCGC_C

Cuts at:      0      21    5505 
   Size:         21    5484
 
KpnI G_GTAC'C

Cuts at:      0     340    2338    5505 
   Size:        340    1998    3167

  Fragments arranged by size: 

                3167    1998     340
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     395    2181    3609    5328    5505 
   Size:        395    1786    1428    1719     177

  Fragments arranged by size: 

                1786    1719    1428     395     177
 
MscI TGG'CCA

Cuts at:      0    3304    5505 
   Size:       3304    2201
 
MslI CAynn'nnrTG

Cuts at:      0    1279    1330    1336    1781    1825    3773    4780    5210 
   Size:       1279      51       6     445      44    1948    1007     430

Cuts at:   5210    5505 
   Size:        295

  Fragments arranged by size: 

                1948    1279    1007     445     430     295      51      44
                   6
 
MspA1I CmG'CkG

Cuts at:      0     852    1018    2512    2866    4798    5034    5314    5505 
   Size:        852     166    1494     354    1932     236     280     191

  Fragments arranged by size: 

                1932    1494     852     354     280     236     191     166
            
 
NarI GG'CG_CC

Cuts at:      0      22    5505 
   Size:         22    5483
 
NcoI C'CATG_G

Cuts at:      0     446    5505 
   Size:        446    5059
 
NdeI CA'TA_TG

Cuts at:      0    2399    4749    5505 
   Size:       2399    2350     756

  Fragments arranged by size: 

                2399    2350     756
 
NheI G'CTAG_C

Cuts at:      0    5397    5505 
   Size:       5397     108
 
NruI TCG'CGA

Cuts at:      0     181    5505 
   Size:        181    5324
 
NsiI A_TGCA'T

Cuts at:      0    3866    5505 
   Size:       3866    1639
 
NspI r_CATG'y

Cuts at:      0    1272    1335    1525    2432    3772    3868    4038    5505 
   Size:       1272      63     190     907    1340      96     170    1467

  Fragments arranged by size: 

                1467    1340    1272     907     190     170      96      63
            
 
PciI A'CATG_T

Cuts at:      0    1331    3768    5505 
   Size:       1331    2437    1737

  Fragments arranged by size: 

                2437    1737    1331
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4846    5505 
   Size:       4846     659
 
PfoI T'CCnGG_A

Cuts at:      0      56    1536    5505 
   Size:         56    1480    3969

  Fragments arranged by size: 

                3969    1480      56
 
PpuMI rG'GwC_Cy

Cuts at:      0    2209    5505 
   Size:       2209    3296
 
PshAI GACnn'nnGTC

Cuts at:      0    3905    5505 
   Size:       3905    1600
 
PsiI TTA'TAA

Cuts at:      0     473    1703    5505 
   Size:        473    1230    3802

  Fragments arranged by size: 

                3802    1230     473
 
PspXI vC'TCGA_Gb

Cuts at:      0    1255    5505 
   Size:       1255    4250
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2003    2035    5505 
   Size:       2003      32    3470

  Fragments arranged by size: 

                3470    2003      32
 
PstI C_TGCA'G

Cuts at:      0    1911    4648    5504    5505 
   Size:       1911    2737     856       1

  Fragments arranged by size: 

                2737    1911     856       1
 
PvuII CAG'CTG

Cuts at:      0    1018    2512    2866    4798    5314    5505 
   Size:       1018    1494     354    1932     516     191

  Fragments arranged by size: 

                1932    1494    1018     516     354     191
 
SacI G_AGCT'C

Cuts at:      0    3276    5505 
   Size:       3276    2229
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2864    5505 
   Size:       2864    2641
 
SbfI CC_TGCA'GG

Cuts at:      0    1911    5505 
   Size:       1911    3594
 
SexAI A'CCwGG_T

Cuts at:      0    3216    3509    5505 
   Size:       3216     293    1996

  Fragments arranged by size: 

                3216    1996     293
 
SfcI C'TryA_G

Cuts at:      0    1197    1454    1907    2147    2438    2618    2723    4644 
   Size:       1197     257     453     240     291     180     105    1921

Cuts at:   4644    4691    5156    5500    5505 
   Size:         47     465     344       5

  Fragments arranged by size: 

                1921    1197     465     453     344     291     257     240
                 180     105      47       5
 
SfoI GGC'GCC

Cuts at:      0      23    5505 
   Size:         23    5482
 
SmlI C'TyrA_G

Cuts at:      0    1255    1622    2664    3659    3903    5505 
   Size:       1255     367    1042     995     244    1602

  Fragments arranged by size: 

                1602    1255    1042     995     367     244
 
SspI AAT'ATT

Cuts at:      0    1011    2580    3449    5324    5334    5344    5505 
   Size:       1011    1569     869    1875      10      10     161

  Fragments arranged by size: 

                1875    1569    1011     869     161      10      10
 
StuI AGG'CCT

Cuts at:      0     992    5505 
   Size:        992    4513
 
StyI C'CwwG_G

Cuts at:      0     446    3492    5505 
   Size:        446    3046    2013

  Fragments arranged by size: 

                3046    2013     446
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     109    1424    4258    4499    5505 
   Size:        109    1315    2834     241    1006

  Fragments arranged by size: 

                2834    1315    1006     241     109
 
TatI w'GTAC_w

Cuts at:      0    1829    2010    2242    2412    2431    2598    3081    3105 
   Size:       1829     181     232     170      19     167     483      24

Cuts at:   3105    3417    3639    3688    3893    4752    4800    5505 
   Size:        312     222      49     205     859      48     705

  Fragments arranged by size: 

                1829     859     705     483     312     232     222     205
                 181     170     167      49      48      24      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1399    1431    5505 
   Size:       1399      32    4074

  Fragments arranged by size: 

                4074    1399      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3499    5409    5505 
   Size:       3499    1910      96

  Fragments arranged by size: 

                3499    1910      96
 
XhoI C'TCGA_G

Cuts at:      0    1255    1622    5505 
   Size:       1255     367    3883

  Fragments arranged by size: 

                3883    1255     367
 
XmnI GAAnn'nnTTC

Cuts at:      0     243     885    5505 
   Size:        243     642    4620

  Fragments arranged by size: 

                4620     642     243
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AhdI     AlfI     AloI
    AlwNI     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NruI     NsiI     NspI     PciI    PflMI     PfoI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI    PvuII     SacI     SapI
     SbfI    SexAI     SfcI     SfoI     SmlI     SspI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AleI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BcgI     BglI    BglII     BplI
    BsaAI    BsiEI    BsiWI    BsmBI    BsrFI   BssHII    BstXI    BtgZI
     ClaI     DrdI     EagI     FalI     FseI     FspI    FspAI     HpaI
     MfeI     MluI     NaeI   NgoMIV     NotI     PacI     PasI     PmeI
     PmlI     PpiI   PspOMI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SwaI  Tth111I     XbaI     XmaI     ZraI
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