Restriction map for pF1KE0095
 (Linear) (Six-base) MAPSORT of: KIEE0095.seq  Check: 8277  from: 1  to: 3783



KIEE0095.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3009    3783 
   Size:       3009     774
 
AatII G_ACGT'C

Cuts at:      0    1751    3783 
   Size:       1751    2032
 
AccI GT'mk_AC

Cuts at:      0    2270    3783 
   Size:       2270    1513
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267    1147    1601    2214    3783 
   Size:        267     880     454     613    1569

  Fragments arranged by size: 

                1569     880     613     454     267
 
AfeI AGC'GCT

Cuts at:      0     694    3783 
   Size:        694    3089
 
AflIII A'CryG_T

Cuts at:      0    2555    2630    3668    3692    3783 
   Size:       2555      75    1038      24      91

  Fragments arranged by size: 

                2555    1038      91      75      24
 
AgeI A'CCGG_T

Cuts at:      0    3634    3783 
   Size:       3634     149
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1752    3783 
   Size:       1752    2031
 
AleI CACnn'nnGTG

Cuts at:      0    1607    1715    2051    2344    2530    3783 
   Size:       1607     108     336     293     186    1253

  Fragments arranged by size: 

                1607    1253     336     293     186     108
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      65    3783 
   Size:         65    3718
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1351    1383    3783 
   Size:       1351      32    2400

  Fragments arranged by size: 

                2400    1351      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     204     215    1122    1467    2699    3681    3783 
   Size:        204      11     907     345    1232     982     102

  Fragments arranged by size: 

                1232     982     907     345     204     102      11
 
ApaI G_GGCC'C

Cuts at:      0     596    3773    3783 
   Size:        596    3177      10

  Fragments arranged by size: 

                3177     596      10
 
ApaLI G'TGCA_C

Cuts at:      0    1452    3783 
   Size:       1452    2331
 
ApoI r'AATT_y

Cuts at:      0    1691    3783 
   Size:       1691    2092
 
AvaI C'yCGr_G

Cuts at:      0      27      65     136     169     383     576     582    1232 
   Size:         27      38      71      33     214     193       6     650

Cuts at:   1232    1569    2719    3783 
   Size:        337    1150    1064

  Fragments arranged by size: 

                1150    1064     650     337     214     193      71      38
                  33      27       6
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3623    3656    3783 
   Size:       3623      33     127

  Fragments arranged by size: 

                3623     127      33
 
BanI G'GyrC_C

Cuts at:      0      54      60     387     526     616    2284    2844    3783 
   Size:         54       6     327     139      90    1668     560     939

  Fragments arranged by size: 

                1668     939     560     327     139      90      54       6
            
 
BanII G_rGCy'C

Cuts at:      0      25     145     583     596    1490    2822    3185    3536 
   Size:         25     120     438      13     894    1332     363     351

Cuts at:   3536    3773    3783 
   Size:        237      10

  Fragments arranged by size: 

                1332     894     438     363     351     237     120      25
                  13      10
 
BbeI G_GCGC'C

Cuts at:      0      58      64     391     530     620    3783 
   Size:         58       6     327     139      90    3163

  Fragments arranged by size: 

                3163     327     139      90      58       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      52    2102    2304    3783 
   Size:         52    2050     202    1479

  Fragments arranged by size: 

                2050    1479     202      52
 
BbvCI CC'TCA_GC

Cuts at:      0    1199    3783 
   Size:       1199    2584
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1152    1186    1694    1728    3783 
   Size:       1152      34     508      34    2055

  Fragments arranged by size: 

                2055    1152     508      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      29     820    2293    3783 
   Size:         29     791    1473    1490

  Fragments arranged by size: 

                1490    1473     791      29
 
BlpI GC'TnA_GC

Cuts at:      0     247    1054    2027    2368    2746    3783 
   Size:        247     807     973     341     378    1037

  Fragments arranged by size: 

                1037     973     807     378     341     247
 
Bme1580I G_kGCm'C

Cuts at:      0     596    1456    2006    2351    2932    3573    3773    3783 
   Size:        596     860     550     345     581     641     200      10

  Fragments arranged by size: 

                 860     641     596     581     550     345     200      10
            
 
BmgBI CAC'GTC

Cuts at:      0    2208    3141    3783 
   Size:       2208     933     642

  Fragments arranged by size: 

                2208     933     642
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1443    2028    2919    3783 
   Size:       1443     585     891     864

  Fragments arranged by size: 

                1443     891     864     585
 
BmtI G_CTAG'C

Cuts at:      0    1774    3783 
   Size:       1774    2009
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1659    3783 
   Size:       1659    2124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     538     697     781    2454    2556    3280    3376 
   Size:        499      39     159      84    1673     102     724      96

Cuts at:   3376    3724    3783 
   Size:        348      59

  Fragments arranged by size: 

                1673     724     499     348     159     102      96      84
                  59      39
 
Bpu10I CC'TnA_GC

Cuts at:      0    1199    2933    3783 
   Size:       1199    1734     850

  Fragments arranged by size: 

                1734    1199     850
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1879    3783 
   Size:       1879    1904
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1209    1222    1732    1906    3783 
   Size:       1209      13     510     174    1877

  Fragments arranged by size: 

                1877    1209     510     174      13
 
BsaAI yAC'GTr

Cuts at:      0    1534    2564    2640    2829    3695    3783 
   Size:       1534    1030      76     189     866      88

  Fragments arranged by size: 

                1534    1030     866     189      88      76
 
BsaBI GATnn'nnATC

Cuts at:      0     844    1381    2341    3526    3783 
   Size:        844     537     960    1185     257

  Fragments arranged by size: 

                1185     960     844     537     257
 
BsaHI Gr'CG_yC

Cuts at:      0      55      61     388     527     617    1444    1748    2174 
   Size:         55       6     327     139      90     827     304     426

Cuts at:   2174    2968    3783 
   Size:        794     815

  Fragments arranged by size: 

                 827     815     794     426     327     304     139      90
                  55       6
 
BsaWI w'CCGG_w

Cuts at:      0    3634    3783 
   Size:       3634     149
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     670     700    2905    2935    3783 
   Size:        670      30    2205      30     848

  Fragments arranged by size: 

                2205     848     670      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     496    3085    3094    3103    3783 
   Size:        496    2589       9       9     680

  Fragments arranged by size: 

                2589     680     496       9       9
 
BseYI C'CCAG_C

Cuts at:      0    2583    3014    3783 
   Size:       2583     431     769

  Fragments arranged by size: 

                2583     769     431
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1146    1167    1442    1917    2992    3103    3783 
   Size:       1146      21     275     475    1075     111     680

  Fragments arranged by size: 

                1146    1075     680     475     275     111      21
 
BsiEI CG_ry'CG

Cuts at:      0     336     509     827    2277    2654    3648    3783 
   Size:        336     173     318    1450     377     994     135

  Fragments arranged by size: 

                1450     994     377     336     318     173     135
 
BsiHKAI G_wGCw'C

Cuts at:      0     752    1055    1067    1456    1490    1904    2207    2606 
   Size:        752     303      12     389      34     414     303     399

Cuts at:   2606    2804    2822    2834    3185    3503    3783 
   Size:        198      18      12     351     318     280

  Fragments arranged by size: 

                 752     414     399     389     351     318     303     303
                 280     198      34      18      12      12
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1572    3783 
   Size:       1572    2211
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     145     583     596     752    1055    1067    1456 
   Size:         25     120     438      13     156     303      12     389

Cuts at:   1456    1490    1904    2006    2207    2351    2606    2804    2822 
   Size:         34     414     102     201     144     255     198      18

Cuts at:   2822    2834    2932    3185    3503    3536    3573    3773    3783 
   Size:         12      98     253     318      33      37     200      10

  Fragments arranged by size: 

                 438     414     389     318     303     255     253     201
                 200     198     156     144     120     102      98      37
                  34      33      25      18      13      12      12      10
            
 
BspHI T'CATG_A

Cuts at:      0    1472    3783 
   Size:       1472    2311
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2256    2588    2885    3009    3014    3783 
   Size:       2256     332     297     124       5     769

  Fragments arranged by size: 

                2256     769     332     297     124       5
 
BsrBI CCG'CTC

Cuts at:      0      86     251     293     486     650     681     795    3148 
   Size:         86     165      42     193     164      31     114    2353

Cuts at:   3148    3168    3777    3783 
   Size:         20     609       6

  Fragments arranged by size: 

                2353     609     193     165     164     114      86      42
                  31      20       6
 
BsrDI GCAATG_nn'

Cuts at:      0    3180    3783 
   Size:       3180     603
 
BsrFI r'CCGG_y

Cuts at:      0      57     347     523    3107    3634    3783 
   Size:         57     290     176    2584     527     149

  Fragments arranged by size: 

                2584     527     290     176     149      57
 
BssHII G'CGCG_C

Cuts at:      0     501    3783 
   Size:        501    3282
 
BssSI C'ACGA_G

Cuts at:      0     751    3783 
   Size:        751    3032
 
BstEII G'GTnAC_C

Cuts at:      0     180    2883    3657    3783 
   Size:        180    2703     774     126

  Fragments arranged by size: 

                2703     774     180     126
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     735    1173    1647    1782    2018    2053    2529    3141 
   Size:        735     438     474     135     236      35     476     612

Cuts at:   3141    3624    3783 
   Size:        483     159

  Fragments arranged by size: 

                 735     612     483     476     474     438     236     159
                 135      35
 
BstYI r'GATC_y

Cuts at:      0    1541    3783 
   Size:       1541    2242
 
Bsu36I CC'TnA_GG

Cuts at:      0    2135    3783 
   Size:       2135    1648
 
BtgI C'CryG_G

Cuts at:      0     150     920    1322    3548    3783 
   Size:        150     770     402    2226     235

  Fragments arranged by size: 

                2226     770     402     235     150
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     900    1074    2687    3213    3294    3783 
   Size:        900     174    1613     526      81     489

  Fragments arranged by size: 

                1613     900     526     489     174      81
 
BtsI GCAGTG_nn'

Cuts at:      0    1055    1454    1602    1710    2455    3029    3783 
   Size:       1055     399     148     108     745     574     754

  Fragments arranged by size: 

                1055     754     745     574     399     148     108
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2930    2965    3783 
   Size:       2930      35     818

  Fragments arranged by size: 

                2930     818      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2881    3783 
   Size:       2881     902
 
EaeI y'GGCC_r

Cuts at:      0     206     266     365     506     737     812     824     929 
   Size:        206      60      99     141     231      75      12     105

Cuts at:    929    1085    1511    2651    2810    3143    3452    3554    3614 
   Size:        156     426    1140     159     333     309     102      60

Cuts at:   3614    3783 
   Size:        169

  Fragments arranged by size: 

                1140     426     333     309     231     206     169     159
                 156     141     105     102      99      75      60      60
                  12
 
EagI C'GGCC_G

Cuts at:      0     506     824    2651    3783 
   Size:        506     318    1827    1132

  Fragments arranged by size: 

                1827    1132     506     318
 
EarI CTCTTCn'nnn_

Cuts at:      0    1495    1946    2323    2449    2866    3783 
   Size:       1495     451     377     126     417     917

  Fragments arranged by size: 

                1495     917     451     417     377     126
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     291     586     694    1062    2659    3783 
   Size:        291     295     108     368    1597    1124

  Fragments arranged by size: 

                1597    1124     368     295     291     108
 
EcoICRI GAG'CTC

Cuts at:      0    1488    2820    3183    3783 
   Size:       1488    1332     363     600

  Fragments arranged by size: 

                1488    1332     600     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     499     538     697     781    1147    1601    2214 
   Size:        267     232      39     159      84     366     454     613

Cuts at:   2214    2454    2556    3280    3376    3724    3783 
   Size:        240     102     724      96     348      59

  Fragments arranged by size: 

                 724     613     454     366     348     267     240     232
                 159     102      96      84      59      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3133    3783 
   Size:       3133     650
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73     592    2577    2918    3769    3770    3783 
   Size:         73     519    1985     341     851       1      13

  Fragments arranged by size: 

                1985     851     519     341      73      13       1
 
EcoRI G'AATT_C

Cuts at:      0    1691    3783 
   Size:       1691    2092
 
FspI TGC'GCA

Cuts at:      0    1036    1099    3783 
   Size:       1036      63    2684

  Fragments arranged by size: 

                2684    1036      63
 
FspAI rTGC'GCAy

Cuts at:      0    1036    3783 
   Size:       1036    2747
 
HaeII r_GCGC'y

Cuts at:      0      58      64     204     391     530     620     696     974 
   Size:         58       6     140     187     139      90      76     278

Cuts at:    974    2034    3783 
   Size:       1060    1749

  Fragments arranged by size: 

                1749    1060     278     187     140     139      90      76
                  58       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     565     597    1226    1258    1738    1770    3783 
   Size:        565      32     629      32     480      32    2013

  Fragments arranged by size: 

                2013     629     565     480      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2553    2628    2658    3783 
   Size:       2553      75      30    1125

  Fragments arranged by size: 

                2553    1125      75      30
 
KasI G'GCGC_C

Cuts at:      0      54      60     387     526     616    3783 
   Size:         54       6     327     139      90    3167

  Fragments arranged by size: 

                3167     327     139      90      54       6
 
MluI A'CGCG_T

Cuts at:      0    2630    3783 
   Size:       2630    1153
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     647    3783 
   Size:        647    3136
 
MscI TGG'CCA

Cuts at:      0     208    1087    2812    3616    3783 
   Size:        208     879    1725     804     167

  Fragments arranged by size: 

                1725     879     804     208     167
 
MslI CAynn'nnrTG

Cuts at:      0     733     739     853     919    1327    1607    1627    1684 
   Size:        733       6     114      66     408     280      20      57

Cuts at:   1684    1715    2051    2344    2362    2530    2827    3385    3667 
   Size:         31     336     293      18     168     297     558     282

Cuts at:   3667    3783 
   Size:        116

  Fragments arranged by size: 

                 733     558     408     336     297     293     282     280
                 168     116     114      66      57      31      20      18
                   6
 
MspA1I CmG'CkG

Cuts at:      0     152     215     246     354     371     801    1058    1266 
   Size:        152      63      31     108      17     430     257     208

Cuts at:   1266    1700    2783    3354    3702    3783 
   Size:        434    1083     571     348      81

  Fragments arranged by size: 

                1083     571     434     430     348     257     208     152
                 108      81      63      31      17
 
NaeI GCC'GGC

Cuts at:      0      59     349     525    3783 
   Size:         59     290     176    3258

  Fragments arranged by size: 

                3258     290     176      59
 
NarI GG'CG_CC

Cuts at:      0      55      61     388     527     617    3783 
   Size:         55       6     327     139      90    3166

  Fragments arranged by size: 

                3166     327     139      90      55       6
 
NcoI C'CATG_G

Cuts at:      0    1322    3783 
   Size:       1322    2461
 
NgoMIV G'CCGG_C

Cuts at:      0      57     347     523    3783 
   Size:         57     290     176    3260

  Fragments arranged by size: 

                3260     290     176      57
 
NheI G'CTAG_C

Cuts at:      0    1770    3783 
   Size:       1770    2013
 
NotI GC'GGCC_GC

Cuts at:      0     824    3783 
   Size:        824    2959
 
NspI r_CATG'y

Cuts at:      0    1041    1683    2559    2941    3672    3783 
   Size:       1041     642     876     382     731     111

  Fragments arranged by size: 

                1041     876     731     642     382     111
 
PasI CC'CwG_GG

Cuts at:      0     612    3783 
   Size:        612    3171
 
PciI A'CATG_T

Cuts at:      0    2555    3668    3783 
   Size:       2555    1113     115

  Fragments arranged by size: 

                2555    1113     115
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     215     942    1870    3021    3681    3783 
   Size:        215     727     928    1151     660     102

  Fragments arranged by size: 

                1151     928     727     660     215     102
 
PfoI T'CCnGG_A

Cuts at:      0    1223    1652    2470    3783 
   Size:       1223     429     818    1313

  Fragments arranged by size: 

                1313    1223     818     429
 
PmlI CAC'GTG

Cuts at:      0    2564    2640    3783 
   Size:       2564      76    1143

  Fragments arranged by size: 

                2564    1143      76
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3328    3360    3783 
   Size:       3328      32     423

  Fragments arranged by size: 

                3328     423      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2577    2918    3783 
   Size:       2577     341     865

  Fragments arranged by size: 

                2577     865     341
 
PshAI GACnn'nnGTC

Cuts at:      0     332    3783 
   Size:        332    3451
 
PspOMI G'GGCC_C

Cuts at:      0     592    3769    3783 
   Size:        592    3177      14

  Fragments arranged by size: 

                3177     592      14
 
PstI C_TGCA'G

Cuts at:      0     641    2462    2777    3062    3098    3783 
   Size:        641    1821     315     285      36     685

  Fragments arranged by size: 

                1821     685     641     315     285      36
 
PvuII CAG'CTG

Cuts at:      0     801    2783    3354    3702    3783 
   Size:        801    1982     571     348      81

  Fragments arranged by size: 

                1982     801     571     348      81
 
SacI G_AGCT'C

Cuts at:      0    1490    2822    3185    3783 
   Size:       1490    1332     363     598

  Fragments arranged by size: 

                1490    1332     598     363
 
SacII CC_GC'GG

Cuts at:      0     153    3783 
   Size:        153    3630
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1495    2449    2866    3783 
   Size:       1495     954     417     917

  Fragments arranged by size: 

                1495     954     917     417
 
ScaI AGT'ACT

Cuts at:      0    2257    2725    3783 
   Size:       2257     468    1058

  Fragments arranged by size: 

                2257    1058     468
 
SexAI A'CCwGG_T

Cuts at:      0    2060    3674    3783 
   Size:       2060    1614     109

  Fragments arranged by size: 

                2060    1614     109
 
SfcI C'TryA_G

Cuts at:      0     637    2458    2661    2733    2773    3058    3094    3783 
   Size:        637    1821     203      72      40     285      36     689

  Fragments arranged by size: 

                1821     689     637     285     203      72      40      36
            
 
SfoI GGC'GCC

Cuts at:      0      56      62     389     528     618    3783 
   Size:         56       6     327     139      90    3165

  Fragments arranged by size: 

                3165     327     139      90      56       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0      57    3634    3783 
   Size:         57    3577     149

  Fragments arranged by size: 

                3577     149      57
 
SmaI CCC'GGG

Cuts at:      0      29      67     138     171     385    3783 
   Size:         29      38      71      33     214    3398

  Fragments arranged by size: 

                3398     214      71      38      33      29
 
SmlI C'TyrA_G

Cuts at:      0    1894    3783 
   Size:       1894    1889
 
SnaBI TAC'GTA

Cuts at:      0    1534    3783 
   Size:       1534    2249
 
SphI G_CATG'C

Cuts at:      0    1041    2941    3783 
   Size:       1041    1900     842

  Fragments arranged by size: 

                1900    1041     842
 
StuI AGG'CCT

Cuts at:      0    3100    3783 
   Size:       3100     683
 
StyI C'CwwG_G

Cuts at:      0    1322    1545    1722    1820    2069    2102    2402    2945 
   Size:       1322     223     177      98     249      33     300     543

Cuts at:   2945    3134    3783 
   Size:        189     649

  Fragments arranged by size: 

                1322     649     543     300     249     223     189     177
                  98      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1165    1201    1951    2041    2186    2291    3783 
   Size:       1165      36     750      90     145     105    1492

  Fragments arranged by size: 

                1492    1165     750     145     105      90      36
 
TatI w'GTAC_w

Cuts at:      0    1707    2255    2723    3488    3783 
   Size:       1707     548     468     765     295

  Fragments arranged by size: 

                1707     765     548     468     295
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1176    1208    3783 
   Size:       1176      32    2575

  Fragments arranged by size: 

                2575    1176      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1786    2580    3471    3783 
   Size:       1786     794     891     312

  Fragments arranged by size: 

                1786     891     794     312
 
XmaI C'CCGG_G

Cuts at:      0      27      65     136     169     383    3783 
   Size:         27      38      71      33     214    3400

  Fragments arranged by size: 

                3400     214      71      38      33      27
 
ZraI GAC'GTC

Cuts at:      0    1749    3783 
   Size:       1749    2034
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    FspAI    HaeII    Hin4I   HincII     KasI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI    SacII     SapI     ScaI
    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI    SnaBI     SphI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AscI     AseI    AsiSI    AvrII    BamHI
    BciVI     BclI    BfrBI    BglII    BsiWI     BsmI    BspEI    BsrGI
   BstAPI    BstBI  BstZ17I     ClaI     DraI   DraIII    EcoRV     FalI
     FseI  HindIII     HpaI     KpnI     MfeI     NdeI     NruI     NsiI
     PacI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     SbfI     SfiI     SgfI     SpeI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmnI
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