Restriction map for pF1KE0092
 (Linear) (Six-base) MAPSORT of: KIEE0092.seq  Check: 7496  from: 1  to: 5715



KIEE0092.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      60    5169    5715 
   Size:         60    5109     546

  Fragments arranged by size: 

                5109     546      60
 
AatII G_ACGT'C

Cuts at:      0    3369    5715 
   Size:       3369    2346
 
AccI GT'mk_AC

Cuts at:      0    1959    5715 
   Size:       1959    3756
 
Acc65I G'GTAC_C

Cuts at:      0     457    4799    5486    5715 
   Size:        457    4342     687     229

  Fragments arranged by size: 

                4342     687     457     229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     656     760    1035    1187    1448    1812    1818 
   Size:        182     474     104     275     152     261     364       6

Cuts at:   1818    2323    2731    3101    3646    3694    3700    3706    5651 
   Size:        505     408     370     545      48       6       6    1945

Cuts at:   5651    5715 
   Size:         64

  Fragments arranged by size: 

                1945     545     505     474     408     370     364     275
                 261     182     152     104      64      48       6       6
                   6
 
AflII C'TTAA_G

Cuts at:      0    3613    5715 
   Size:       3613    2102
 
AflIII A'CryG_T

Cuts at:      0    4615    5032    5715 
   Size:       4615     417     683

  Fragments arranged by size: 

                4615     683     417
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2808    5715 
   Size:       2808    2907
 
AleI CACnn'nnGTG

Cuts at:      0    5387    5715 
   Size:       5387     328
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2930    3325    4499    5715 
   Size:       2930     395    1174    1216

  Fragments arranged by size: 

                2930    1216    1174     395
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3111    3143    5715 
   Size:       3111      32    2572

  Fragments arranged by size: 

                3111    2572      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1237    1507    2502    3928    4027    5715 
   Size:       1237     270     995    1426      99    1688

  Fragments arranged by size: 

                1688    1426    1237     995     270      99
 
ApaI G_GGCC'C

Cuts at:      0    3564    5715 
   Size:       3564    2151
 
ApoI r'AATT_y

Cuts at:      0     522    5191    5715 
   Size:        522    4669     524

  Fragments arranged by size: 

                4669     524     522
 
AvaI C'yCGr_G

Cuts at:      0     348    3557    3563    3589    4370    4942    5715 
   Size:        348    3209       6      26     781     572     773

  Fragments arranged by size: 

                3209     781     773     572     348      26       6
 
AvrII C'CTAG_G

Cuts at:      0    1119    3604    5715 
   Size:       1119    2485    2111

  Fragments arranged by size: 

                2485    2111    1119
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4783    4816    5715 
   Size:       4783      33     899

  Fragments arranged by size: 

                4783     899      33
 
BanI G'GyrC_C

Cuts at:      0     457     915    4719    4799    4870    4882    4931    4949 
   Size:        457     458    3804      80      71      12      49      18

Cuts at:   4949    5486    5715 
   Size:        537     229

  Fragments arranged by size: 

                3804     537     458     457     229      80      71      49
                  18      12
 
BanII G_rGCy'C

Cuts at:      0     902    2945    3564    4472    4814    5345    5715 
   Size:        902    2043     619     908     342     531     370

  Fragments arranged by size: 

                2043     908     902     619     531     370     342
 
BbeI G_GCGC'C

Cuts at:      0    4935    5715 
   Size:       4935     780
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     872     893    1386    1651    4531    4649    5715 
   Size:        872      21     493     265    2880     118    1066

  Fragments arranged by size: 

                2880    1066     872     493     265     118      21
 
BbvCI CC'TCA_GC

Cuts at:      0    1419    3704    3790    4052    4449    4724    5715 
   Size:       1419    2285      86     262     397     275     991

  Fragments arranged by size: 

                2285    1419     991     397     275     262      86
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     646    4316    4387    5715 
   Size:        646    3670      71    1328

  Fragments arranged by size: 

                3670    1328     646      71
 
BclI T'GATC_A

Cuts at:      0     855    1239    1778    5715 
   Size:        855     384     539    3937

  Fragments arranged by size: 

                3937     855     539     384
 
BglI GCCn_nnn'nGGC

Cuts at:      0    4818    5715 
   Size:       4818     897
 
BglII A'GATC_T

Cuts at:      0    2762    5715 
   Size:       2762    2953
 
BlpI GC'TnA_GC

Cuts at:      0    3674    3817    4855    5174    5715 
   Size:       3674     143    1038     319     541

  Fragments arranged by size: 

                3674    1038     541     319     143
 
Bme1580I G_kGCm'C

Cuts at:      0    3564    4875    4885    5651    5715 
   Size:       3564    1311      10     766      64

  Fragments arranged by size: 

                3564    1311     766      64      10
 
BmgBI CAC'GTC

Cuts at:      0    1572    5715 
   Size:       1572    4143
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     336    2534    3080    3384    3778    4226    5715 
   Size:        336    2198     546     304     394     448    1489

  Fragments arranged by size: 

                2198    1489     546     448     394     336     304
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1612    3842    4323    4683    5643    5715 
   Size:       1612    2230     481     360     960      72

  Fragments arranged by size: 

                2230    1612     960     481     360      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     916    1310    1334    1370    1394    1607    2107 
   Size:        213     703     394      24      36      24     213     500

Cuts at:   2107    3007    3763    4094    4193    4591    4861    5011    5715 
   Size:        900     756     331      99     398     270     150     704

  Fragments arranged by size: 

                 900     756     704     703     500     398     394     331
                 270     213     213     150      99      36      24      24
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1419    1789    3704    3790    4052    4308    4449    4724 
   Size:       1419     370    1915      86     262     256     141     275

Cuts at:   4724    5542    5715 
   Size:        818     173

  Fragments arranged by size: 

                1915    1419     818     370     275     262     256     173
                 141      86
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1012    3941    4339    5715 
   Size:        304     708    2929     398    1376

  Fragments arranged by size: 

                2929    1376     708     398     304
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     116     801    1410    1720    2215    3139    3345    4160 
   Size:        116     685     609     310     495     924     206     815

Cuts at:   4160    4218    5715 
   Size:         58    1497

  Fragments arranged by size: 

                1497     924     815     685     609     495     310     206
                 116      58
 
BsaHI Gr'CG_yC

Cuts at:      0    3366    4932    5280    5715 
   Size:       3366    1566     348     435

  Fragments arranged by size: 

                3366    1566     435     348
 
BsaWI w'CCGG_w

Cuts at:      0    3038    5715 
   Size:       3038    2677
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1717    1747    1909    1939    2764    2794    2980    3010 
   Size:       1717      30     162      30     825      30     186      30

Cuts at:   3010    3111    3141    5413    5443    5715 
   Size:        101      30    2272      30     272

  Fragments arranged by size: 

                2272    1717     825     272     186     162     101      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     216    1641    1930    2125    2371    3157    3190    3451 
   Size:        216    1425     289     195     246     786      33     261

Cuts at:   3451    3931    4056    4579    5581    5698    5715 
   Size:        480     125     523    1002     117      17

  Fragments arranged by size: 

                1425    1002     786     523     480     289     261     246
                 216     195     125     117      33      17
 
BseYI C'CCAG_C

Cuts at:      0     128     253     430    1226    1302    1431    1500    1527 
   Size:        128     125     177     796      76     129      69      27

Cuts at:   1527    2601    2879    2894    2903    3656    3692    3698    3894 
   Size:       1074     278      15       9     753      36       6     196

Cuts at:   3894    4138    4200    4271    4283    5310    5644    5678    5715 
   Size:        244      62      71      12    1027     334      34      37

  Fragments arranged by size: 

                1074    1027     796     753     334     278     244     196
                 177     129     128     125      76      71      69      62
                  37      36      34      27      15      12       9       6
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     992    1921    2702    4135    5715 
   Size:         32     960     929     781    1433    1580

  Fragments arranged by size: 

                1580    1433     960     929     781      32
 
BsiHKAI G_wGCw'C

Cuts at:      0    1826    2945    4309    4664    5715 
   Size:       1826    1119    1364     355    1051

  Fragments arranged by size: 

                1826    1364    1119    1051     355
 
BsmI GAATG_Cn'

Cuts at:      0     596    3112    4919    5715 
   Size:        596    2516    1807     796

  Fragments arranged by size: 

                2516    1807     796     596
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1623    5715 
   Size:       1623    4092
 
Bsp1286I G_dGCh'C

Cuts at:      0     902    1826    2945    3564    4309    4472    4664    4814 
   Size:        902     924    1119     619     745     163     192     150

Cuts at:   4814    4875    4885    5345    5651    5715 
   Size:         61      10     460     306      64

  Fragments arranged by size: 

                1119     924     902     745     619     460     306     192
                 163     150      64      61      10
 
BspEI T'CCGG_A

Cuts at:      0    3038    5715 
   Size:       3038    2677
 
BspHI T'CATG_A

Cuts at:      0    1622    4827    5715 
   Size:       1622    3205     888

  Fragments arranged by size: 

                3205    1622     888
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      60    2702    4491    4811    5169    5715 
   Size:         60    2642    1789     320     358     546

  Fragments arranged by size: 

                2642    1789     546     358     320      60
 
BsrBI CCG'CTC

Cuts at:      0    4947    5535    5715 
   Size:       4947     588     180

  Fragments arranged by size: 

                4947     588     180
 
BsrDI GCAATG_nn'

Cuts at:      0    2533    4002    5000    5715 
   Size:       2533    1469     998     715

  Fragments arranged by size: 

                2533    1469     998     715
 
BsrFI r'CCGG_y

Cuts at:      0     389    3032    5489    5715 
   Size:        389    2643    2457     226

  Fragments arranged by size: 

                2643    2457     389     226
 
BssSI C'ACGA_G

Cuts at:      0    2516    5715 
   Size:       2516    3199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      55    1899    2128    2694    3481    5655    5715 
   Size:         55    1844     229     566     787    2174      60

  Fragments arranged by size: 

                2174    1844     787     566     229      60      55
 
BstEII G'GTnAC_C

Cuts at:      0    2361    5715 
   Size:       2361    3354
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2731    3156    4134    4200    4746    5715 
   Size:       2731     425     978      66     546     969

  Fragments arranged by size: 

                2731     978     969     546     425      66
 
BstYI r'GATC_y

Cuts at:      0     589    1919    2762    2786    2816    4329    5715 
   Size:        589    1330     843      24      30    1513    1386

  Fragments arranged by size: 

                1513    1386    1330     843     589      30      24
 
Bsu36I CC'TnA_GG

Cuts at:      0      60      84    2030    3434    3668    4877    5715 
   Size:         60      24    1946    1404     234    1209     838

  Fragments arranged by size: 

                1946    1404    1209     838     234      60      24
 
BtgI C'CryG_G

Cuts at:      0     470    5094    5715 
   Size:        470    4624     621

  Fragments arranged by size: 

                4624     621     470
 
BtsI GCAGTG_nn'

Cuts at:      0     636    1562    1598    1900    3539    4553    5715 
   Size:        636     926      36     302    1639    1014    1162

  Fragments arranged by size: 

                1639    1162    1014     926     636     302      36
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1657    1692    5715 
   Size:       1657      35    4023

  Fragments arranged by size: 

                4023    1657      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2660    2698    5715 
   Size:       2660      38    3017

  Fragments arranged by size: 

                3017    2660      38
 
EaeI y'GGCC_r

Cuts at:      0    2023    2402    3059    3198    4819    5223    5271    5715 
   Size:       2023     379     657     139    1621     404      48     444

  Fragments arranged by size: 

                2023    1621     657     444     404     379     139      48
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     719    1571    1747    3158    3167    3207    3508    4128 
   Size:        719     852     176    1411       9      40     301     620

Cuts at:   4128    5715 
   Size:       1587

  Fragments arranged by size: 

                1587    1411     852     719     620     301     176      40
                   9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1695    1804    5715 
   Size:       1695     109    3911

  Fragments arranged by size: 

                3911    1695     109
 
EcoICRI GAG'CTC

Cuts at:      0    2943    5715 
   Size:       2943    2772
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     213     656     760     916    1035    1187    1310 
   Size:        182      31     443     104     156     119     152     123

Cuts at:   1310    1334    1370    1394    1448    1607    1812    1818    2107 
   Size:         24      36      24      54     159     205       6     289

Cuts at:   2107    2323    2731    3007    3101    3646    3694    3700    3706 
   Size:        216     408     276      94     545      48       6       6

Cuts at:   3706    3763    4094    4193    4591    4861    5011    5651    5715 
   Size:         57     331      99     398     270     150     640      64

  Fragments arranged by size: 

                 640     545     443     408     398     331     289     276
                 270     216     205     182     159     156     152     150
                 123     119     104      99      94      64      57      54
                  48      36      31      24      24       6       6       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      83    1118    2647    3349    5715 
   Size:         83    1035    1529     702    2366

  Fragments arranged by size: 

                2366    1529    1035     702      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     324    2640    4359    4848    5320    5715 
   Size:         93     231    2316    1719     489     472     395

  Fragments arranged by size: 

                2316    1719     489     472     395     231      93
 
EcoRI G'AATT_C

Cuts at:      0    5191    5715 
   Size:       5191     524
 
EcoRV GAT'ATC

Cuts at:      0    4686    4898    5715 
   Size:       4686     212     817

  Fragments arranged by size: 

                4686     817     212
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1675    5715 
   Size:       1675    4040
 
FspI TGC'GCA

Cuts at:      0    1693    2875    5715 
   Size:       1693    1182    2840

  Fragments arranged by size: 

                2840    1693    1182
 
FspAI rTGC'GCAy

Cuts at:      0    1693    5715 
   Size:       1693    4022
 
HaeII r_GCGC'y

Cuts at:      0    2418    3264    4935    5715 
   Size:       2418     846    1671     780

  Fragments arranged by size: 

                2418    1671     846     780
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1231    1263    1909    1941    2764    2794    2796    2826 
   Size:       1231      32     646      32     823      30       2      30

Cuts at:   2826    2980    3012    3925    3957    4314    4346    4899    4931 
   Size:        154      32     913      32     357      32     553      32

Cuts at:   4931    5413    5445    5715 
   Size:        482      32     270

  Fragments arranged by size: 

                1231     913     823     646     553     482     357     270
                 154      32      32      32      32      32      32      32
                  30      30       2
 
HincII GTy'rAC

Cuts at:      0    1457    1960    5022    5715 
   Size:       1457     503    3062     693

  Fragments arranged by size: 

                3062    1457     693     503
 
KasI G'GCGC_C

Cuts at:      0    4931    5715 
   Size:       4931     784
 
KpnI G_GTAC'C

Cuts at:      0     461    4803    5490    5715 
   Size:        461    4342     687     225

  Fragments arranged by size: 

                4342     687     461     225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1540    1692    1833    3557    4322    4694    5715 
   Size:       1540     152     141    1724     765     372    1021

  Fragments arranged by size: 

                1724    1540    1021     765     372     152     141
 
MscI TGG'CCA

Cuts at:      0    2404    3061    4821    5225    5715 
   Size:       2404     657    1760     404     490

  Fragments arranged by size: 

                2404    1760     657     490     404
 
MslI CAynn'nnrTG

Cuts at:      0     644    5387    5715 
   Size:        644    4743     328

  Fragments arranged by size: 

                4743     644     328
 
MspA1I CmG'CkG

Cuts at:      0    1507    3190    4275    4453    4578    4989    5678    5715 
   Size:       1507    1683    1085     178     125     411     689      37

  Fragments arranged by size: 

                1683    1507    1085     689     411     178     125      37
            
 
NaeI GCC'GGC

Cuts at:      0     391    3034    5715 
   Size:        391    2643    2681

  Fragments arranged by size: 

                2681    2643     391
 
NarI GG'CG_CC

Cuts at:      0    4932    5715 
   Size:       4932     783
 
NcoI C'CATG_G

Cuts at:      0     470    5715 
   Size:        470    5245
 
NdeI CA'TA_TG

Cuts at:      0    2841    4902    5715 
   Size:       2841    2061     813

  Fragments arranged by size: 

                2841    2061     813
 
NgoMIV G'CCGG_C

Cuts at:      0     389    3032    5715 
   Size:        389    2643    2683

  Fragments arranged by size: 

                2683    2643     389
 
NspI r_CATG'y

Cuts at:      0    4619    5036    5715 
   Size:       4619     417     679

  Fragments arranged by size: 

                4619     679     417
 
PasI CC'CwG_GG

Cuts at:      0    3345    5715 
   Size:       3345    2370
 
PciI A'CATG_T

Cuts at:      0    4615    5032    5715 
   Size:       4615     417     683

  Fragments arranged by size: 

                4615     683     417
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     931    5006    5221    5715 
   Size:        931    4075     215     494

  Fragments arranged by size: 

                4075     931     494     215
 
PfoI T'CCnGG_A

Cuts at:      0    3740    3879    4379    5715 
   Size:       3740     139     500    1336

  Fragments arranged by size: 

                3740    1336     500     139
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3111    3143    5715 
   Size:       3111      32    2572

  Fragments arranged by size: 

                3111    2572      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     324    2640    5715 
   Size:        324    2316    3075

  Fragments arranged by size: 

                3075    2316     324
 
PspOMI G'GGCC_C

Cuts at:      0    3560    5715 
   Size:       3560    2155
 
PspXI vC'TCGA_Gb

Cuts at:      0    4942    5715 
   Size:       4942     773
 
PstI C_TGCA'G

Cuts at:      0    2648    4023    4601    5715 
   Size:       2648    1375     578    1114

  Fragments arranged by size: 

                2648    1375    1114     578
 
PvuII CAG'CTG

Cuts at:      0    1507    3190    4275    4453    4989    5715 
   Size:       1507    1683    1085     178     536     726

  Fragments arranged by size: 

                1683    1507    1085     726     536     178
 
SacI G_AGCT'C

Cuts at:      0    2945    5715 
   Size:       2945    2770
 
SalI G'TCGA_C

Cuts at:      0    1958    5715 
   Size:       1958    3757
 
SapI GCTCTTCn'nnn_

Cuts at:      0     719    5715 
   Size:        719    4996
 
SbfI CC_TGCA'GG

Cuts at:      0    2648    5715 
   Size:       2648    3067
 
SexAI A'CCwGG_T

Cuts at:      0    1729    5715 
   Size:       1729    3986
 
SfcI C'TryA_G

Cuts at:      0    1373    2644    3003    3182    3360    4019    4597    4889 
   Size:       1373    1271     359     179     178     659     578     292

Cuts at:   4889    5715 
   Size:        826

  Fragments arranged by size: 

                1373    1271     826     659     578     359     292     179
                 178
 
SfoI GGC'GCC

Cuts at:      0    4933    5715 
   Size:       4933     782
 
SmaI CCC'GGG

Cuts at:      0    3559    3591    5715 
   Size:       3559      32    2124

  Fragments arranged by size: 

                3559    2124      32
 
SmlI C'TyrA_G

Cuts at:      0     319     991    3613    3956    4354    4942    5715 
   Size:        319     672    2622     343     398     588     773

  Fragments arranged by size: 

                2622     773     672     588     398     343     319
 
SrfI GCCC'GGGC

Cuts at:      0    3559    5715 
   Size:       3559    2156
 
StyI C'CwwG_G

Cuts at:      0     378     470    1119    2000    2190    2261    3604    4133 
   Size:        378      92     649     881     190      71    1343     529

Cuts at:   4133    5715 
   Size:       1582

  Fragments arranged by size: 

                1582    1343     881     649     529     378     190      92
                  71
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     958    1664    1887    2668    2893    5075    5715 
   Size:        958     706     223     781     225    2182     640

  Fragments arranged by size: 

                2182     958     781     706     640     225     223
 
TatI w'GTAC_w

Cuts at:      0    5158    5715 
   Size:       5158     557
 
XbaI T'CTAG_A

Cuts at:      0    1554    5715 
   Size:       1554    4161
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1569    5147    5715 
   Size:       1569    3578     568

  Fragments arranged by size: 

                3578    1569     568
 
XhoI C'TCGA_G

Cuts at:      0    4942    5715 
   Size:       4942     773
 
XmaI C'CCGG_G

Cuts at:      0    3557    3589    5715 
   Size:       3557      32    2126

  Fragments arranged by size: 

                3557    2126      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1652    5715 
   Size:       1652    4063
 
ZraI GAC'GTC

Cuts at:      0    3367    5715 
   Size:       3367    2348
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI    AvrII
     BaeI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI    CspCI     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    FspAI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PpiI    PpuMI   PspOMI    PspXI
     PstI    PvuII     SacI     SalI     SapI     SbfI    SexAI     SfcI
     SfoI     SmaI     SmlI     SrfI     StyI    TaqII     TatI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI     AgeI    ApaLI     AscI     AseI    AsiSI    BamHI
     BcgI    BfrBI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BsrGI
   BssHII    BstBI  BstZ17I    BtgZI     ClaI     DraI   DraIII     EagI
     FseI  HindIII     HpaI     MfeI     MluI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PmlI    PshAI     PsiI     PsrI     PvuI
    RsrII    SacII    SanDI     ScaI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SspI     StuI     SwaI     TstI  Tth111I
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