Restriction map for pF1KE0090
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# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BanI        BbeI        BbvCI       
BcgI        BciVI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FauI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         1192  GTCTAC               GT^[AC][GT]AC
AlwNI          93  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1197  CCTCAGG              CC^T[ACGT]AGG
AvaI          554  CTCGGG               C^[CT]CG[GA]G
BamHI         794  GGATCC               G^GATCC
BanII         931  GAGCTC               G[GA]GC[CT]^C
BbrPI        1168  CACGTG               CAC^GTG
BclI          281  TGATCA               T^GATCA
BcnI          251  CCGGG                CC^[GC]GG
BglI          433  GCCTTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1168  CACGTG               [CT]AC^GT[GA]
BsaBI         532  GATTCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         950  ACCGGA               [AT]^CCGG[AT]
BsgI         1171  GTGCAG               GTGCAG(16/14)
BsmAI         963  GAGAC                GTCTC(1/5)
BsmBI         963  GAGACG               CGTCTC(1/5)
BsoBI         554  CTCGGG               C^[CT]CG[GA]G
BspDI        1327  ATCGAT               AT^CGAT
BspHI         675  TCATGA               T^CATGA
BspMI         969  GCAGGT               ACCTGC(4/8)
BsrI         1100  ACTGG                ACTGG(1/-1)
BstYI         794  GGATCC               [GA]^GATC[CT]
Bsu36I       1197  CCTCAGG              CC^T[ACGT]AGG
Cac8I         438  GCAGGC               GC[ACGT]^[ACGT]GC
CfoI          452  GCGC                 GCG^C
ClaI         1327  ATCGAT               AT^CGAT
Eco81I       1197  CCTCAGG              CC^T[ACGT]AGG
EcoRI         989  GAATTC               G^AATTC
EcoRV         504  GATATC               GAT^ATC
EcoT14I      1162  CCAAGG               C^C[AT][AT]GG
EcoT22I       572  ATGCAT               ATGCA^T
FbaI          281  TGATCA               T^GATCA
HaeII         451  AGCGCC               [GA]GCGC^[CT]
HgaI          907  GACGC                GACGC(5/10)
HhaI          452  GCGC                 GCG^C
HinP1I        452  GCGC                 G^CGC
MamI          532  GATTCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          794  GGATCC               [GA]^GATC[CT]
NciI          251  CCGGG                CC^[GC]GG
NsiI          572  ATGCAT               ATGCA^T
PmaCI        1168  CACGTG               CAC^GTG
PmlI         1168  CACGTG               CAC^GTG
PstI           62  CTGCAG               CTGCA^G
RcaI          675  TCATGA               T^CATGA
SacI          931  GAGCTC               GAGCT^C
SapI          939  GCTCTTC              GCTCTTC(1/4)
SfiI          432  GGCCTTGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI          140  AATATT               AAT^ATT
StyI         1162  CCAAGG               C^C[AT][AT]GG
XhoII         794  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          732  GTAC                 GT^AC
              747  GTAC                
AlwI          795  GATCC                GGATC(4/5)
              794  GGATC               
Asp700I       191  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              819  GAAAGTATTC          
AspHI           7  GTGCTC               G[AT]GC[AT]^C
              931  GAGCTC              
BbsI         1212  GAAGAC               GAAGAC(2/6)
               31  GTCTTC              
BpuAI        1212  GAAGAC               GAAGAC(2/6)
               31  GTCTTC              
BseRI        1133  CTCCTC               GAGGAG(10/8)
              599  GAGGAG              
BsiHKAI         7  GTGCTC               G[AT]GC[AT]^C
              931  GAGCTC              
EarI          328  CTCTTC               CTCTTC(1/4)
              940  CTCTTC              
HapII         251  CCGG                 C^CGG
              951  CCGG                
HpaII         251  CCGG                 C^CGG
              951  CCGG                
Ksp632I       328  CTCTTC               CTCTTC(1/4)
              940  CTCTTC              
MaeII         278  ACGT                 A^CGT
             1169  ACGT                
MspI          251  CCGG                 C^CGG
              951  CCGG                
PleI          561  GACTC                GAGTC(4/5)
              889  GACTC               
RsaI          732  GTAC                 GT^AC
              747  GTAC                
StuI          440  AGGCCT               AGG^CCT
              591  AGGCCT              
XmnI          191  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              819  GAAAGTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        AvaII       
BalI        BbvI        BfaI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoNI       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PpuMI       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      
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