Restriction map for
pF1KE0077
(Linear) (Six-base) MAPSORT of: KIEE0077.seq Check: 1414 from: 1 to: 3609
KIEE0077.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1193 1890 2271 3609
Size: 109 1084 697 381 1338
Fragments arranged by size:
1338 1084 697 381 109
AfeI AGC'GCT
Cuts at: 0 2284 3609
Size: 2284 1325
AleI CACnn'nnGTG
Cuts at: 0 2116 3609
Size: 2116 1493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 1142 1996 2359 3191 3609
Size: 976 166 854 363 832 418
Fragments arranged by size:
976 854 832 418 363 166
AlwNI CAG_nnn'CTG
Cuts at: 0 126 288 342 663 1374 1712 1737 1749
Size: 126 162 54 321 711 338 25 12
Cuts at: 1749 2871 3532 3604 3609
Size: 1122 661 72 5
Fragments arranged by size:
1122 711 661 338 321 162 126 72
54 25 12 5
ApaI G_GGCC'C
Cuts at: 0 204 2454 2790 3346 3609
Size: 204 2250 336 556 263
Fragments arranged by size:
2250 556 336 263 204
ApaLI G'TGCA_C
Cuts at: 0 2824 2919 3609
Size: 2824 95 690
Fragments arranged by size:
2824 690 95
ApoI r'AATT_y
Cuts at: 0 865 3609
Size: 865 2744
AvaI C'yCGr_G
Cuts at: 0 33 2153 2557 2789 3609
Size: 33 2120 404 232 820
Fragments arranged by size:
2120 820 404 232 33
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3352 3385 3609
Size: 3352 33 224
Fragments arranged by size:
3352 224 33
BamHI G'GATC_C
Cuts at: 0 149 648 3609
Size: 149 499 2961
Fragments arranged by size:
2961 499 149
BanI G'GyrC_C
Cuts at: 0 1450 1495 1667 1869 2148 2158 2328 3511
Size: 1450 45 172 202 279 10 170 1183
Cuts at: 3511 3609
Size: 98
Fragments arranged by size:
1450 1183 279 202 172 170 98 45
10
BanII G_rGCy'C
Cuts at: 0 204 604 1328 1897 1904 2454 2564 2790
Size: 204 400 724 569 7 550 110 226
Cuts at: 2790 3346 3542 3609
Size: 556 196 67
Fragments arranged by size:
724 569 556 550 400 226 204 196
110 67 7
BbeI G_GCGC'C
Cuts at: 0 1671 3609
Size: 1671 1938
BbsI GAAGACnn'nnnn_
Cuts at: 0 1626 2761 3609
Size: 1626 1135 848
Fragments arranged by size:
1626 1135 848
BbvCI CC'TCA_GC
Cuts at: 0 931 1648 3609
Size: 931 717 1961
Fragments arranged by size:
1961 931 717
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1485 1519 2910 2944 3609
Size: 1485 34 1391 34 665
Fragments arranged by size:
1485 1391 665 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 199 1093 2326 2623 2815 3609
Size: 199 894 1233 297 192 794
Fragments arranged by size:
1233 894 794 297 199 192
BlpI GC'TnA_GC
Cuts at: 0 2705 3609
Size: 2705 904
Bme1580I G_kGCm'C
Cuts at: 0 204 792 1335 1498 1874 1992 2161 2454
Size: 204 588 543 163 376 118 169 293
Cuts at: 2454 2790 2828 2923 3346 3609
Size: 336 38 95 423 263
Fragments arranged by size:
588 543 423 376 336 293 263 204
169 163 118 95 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1251 1900 2406 2718 3609
Size: 1251 649 506 312 891
Fragments arranged by size:
1251 891 649 506 312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 607 3609
Size: 607 3002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 685 1047 1444 1689 1934 2026 2501
Size: 391 294 362 397 245 245 92 475
Cuts at: 2501 2734 3609
Size: 233 875
Fragments arranged by size:
875 475 397 391 362 294 245 245
233 92
Bpu10I CC'TnA_GC
Cuts at: 0 931 1648 1721 2054 2095 2179 2191 3421
Size: 931 717 73 333 41 84 12 1230
Cuts at: 3421 3609
Size: 188
Fragments arranged by size:
1230 931 717 333 188 84 73 41
12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 2281 3184 3609
Size: 712 1569 903 425
Fragments arranged by size:
1569 903 712 425
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 905 3609
Size: 220 685 2704
Fragments arranged by size:
2704 685 220
BsaAI yAC'GTr
Cuts at: 0 741 3105 3609
Size: 741 2364 504
Fragments arranged by size:
2364 741 504
BsaBI GATnn'nnATC
Cuts at: 0 1732 3609
Size: 1732 1877
BsaHI Gr'CG_yC
Cuts at: 0 1668 2486 3609
Size: 1668 818 1123
Fragments arranged by size:
1668 1123 818
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 383 413 2236 2266 3609
Size: 383 30 1823 30 1343
Fragments arranged by size:
1823 1343 383 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 682 1048 1125 1960 1963 2938 3289
Size: 331 351 366 77 835 3 975 351
Cuts at: 3289 3429 3609
Size: 140 180
Fragments arranged by size:
975 835 366 351 351 331 180 140
77 3
BseYI C'CCAG_C
Cuts at: 0 48 79 309 672 894 1115 1638 1749
Size: 48 31 230 363 222 221 523 111
Cuts at: 1749 2186 2818 2836 3210 3609
Size: 437 632 18 374 399
Fragments arranged by size:
632 523 437 399 374 363 230 222
221 111 48 31 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1099 1593 2260 2329 2641 3609
Size: 742 357 494 667 69 312 968
Fragments arranged by size:
968 742 667 494 357 312 69
BsiEI CG_ry'CG
Cuts at: 0 10 621 3609
Size: 10 611 2988
Fragments arranged by size:
2988 611 10
BsiHKAI G_wGCw'C
Cuts at: 0 176 955 1694 1904 2075 2085 2564 2828
Size: 176 779 739 210 171 10 479 264
Cuts at: 2828 2923 2940 3170 3284 3609
Size: 95 17 230 114 325
Fragments arranged by size:
779 739 479 325 264 230 210 176
171 114 95 17 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2477 3609
Size: 2477 1132
Bsp1286I G_dGCh'C
Cuts at: 0 176 204 604 792 955 1328 1335 1498
Size: 176 28 400 188 163 373 7 163
Cuts at: 1498 1694 1874 1897 1904 1992 2075 2085 2161
Size: 196 180 23 7 88 83 10 76
Cuts at: 2161 2454 2564 2790 2828 2923 2940 3170 3284
Size: 293 110 226 38 95 17 230 114
Cuts at: 3284 3346 3542 3609
Size: 62 196 67
Fragments arranged by size:
400 373 293 230 226 196 196 188
180 176 163 163 114 110 95 88
83 76 67 62 38 28 23 17
10 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 3152 3609
Size: 620 2532 457
Fragments arranged by size:
2532 620 457
BsrBI CCG'CTC
Cuts at: 0 522 3609
Size: 522 3087
BsrDI GCAATG_nn'
Cuts at: 0 1775 2439 3205 3609
Size: 1775 664 766 404
Fragments arranged by size:
1775 766 664 404
BsrFI r'CCGG_y
Cuts at: 0 2316 2809 3609
Size: 2316 493 800
Fragments arranged by size:
2316 800 493
BsrGI T'GTAC_A
Cuts at: 0 3368 3609
Size: 3368 241
BssSI C'ACGA_G
Cuts at: 0 829 3310 3609
Size: 829 2481 299
Fragments arranged by size:
2481 829 299
BstEII G'GTnAC_C
Cuts at: 0 1839 2223 3609
Size: 1839 384 1386
Fragments arranged by size:
1839 1386 384
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1146 2067 3054 3210 3483 3609
Size: 1146 921 987 156 273 126
Fragments arranged by size:
1146 987 921 273 156 126
BstYI r'GATC_y
Cuts at: 0 71 149 648 1121 1733 3386 3609
Size: 71 78 499 473 612 1653 223
Fragments arranged by size:
1653 612 499 473 223 78 71
Bsu36I CC'TnA_GG
Cuts at: 0 1314 3609
Size: 1314 2295
BtgI C'CryG_G
Cuts at: 0 849 1271 2675 3053 3303 3482 3609
Size: 849 422 1404 378 250 179 127
Fragments arranged by size:
1404 849 422 378 250 179 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3004 3609
Size: 3004 605
BtsI GCAGTG_nn'
Cuts at: 0 419 774 1671 1787 2601 3609
Size: 419 355 897 116 814 1008
Fragments arranged by size:
1008 897 814 419 355 116
DraIII CAC_nnn'GTG
Cuts at: 0 787 1869 3609
Size: 787 1082 1740
Fragments arranged by size:
1740 1082 787
EaeI y'GGCC_r
Cuts at: 0 224 618 905 1529 1592 2318 2811 2897
Size: 224 394 287 624 63 726 493 86
Cuts at: 2897 3485 3609
Size: 588 124
Fragments arranged by size:
726 624 588 493 394 287 224 124
86 63
EagI C'GGCC_G
Cuts at: 0 618 3609
Size: 618 2991
EarI CTCTTCn'nnn_
Cuts at: 0 136 1909 2090 2569 2860 3609
Size: 136 1773 181 479 291 749
Fragments arranged by size:
1773 749 479 291 181 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 594 3609
Size: 594 3015
EcoICRI GAG'CTC
Cuts at: 0 1902 2562 3609
Size: 1902 660 1047
Fragments arranged by size:
1902 1047 660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 391 685 1047 1193 1444 1689 1890
Size: 109 282 294 362 146 251 245 201
Cuts at: 1890 1934 2026 2271 2501 2734 3609
Size: 44 92 245 230 233 875
Fragments arranged by size:
875 362 294 282 251 245 245 233
230 201 146 109 92 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1741 2209 3609
Size: 1741 468 1400
Fragments arranged by size:
1741 1400 468
EcoO109I rG'GnC_Cy
Cuts at: 0 123 200 201 676 942 1095 1929 2450
Size: 123 77 1 475 266 153 834 521
Cuts at: 2450 2451 2786 3609
Size: 1 335 823
Fragments arranged by size:
834 823 521 475 335 266 153 123
77 1 1
EcoRV GAT'ATC
Cuts at: 0 630 3609
Size: 630 2979
FseI GG_CCGG'CC
Cuts at: 0 2813 3609
Size: 2813 796
FspI TGC'GCA
Cuts at: 0 2802 3609
Size: 2802 807
HaeII r_GCGC'y
Cuts at: 0 1074 1671 1683 2186 2286 2886 3274 3609
Size: 1074 597 12 503 100 600 388 335
Fragments arranged by size:
1074 600 597 503 388 335 100 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 134 166 359 391 3378 3410 3609
Size: 134 32 193 32 2987 32 199
Fragments arranged by size:
2987 199 193 134 32 32 32
KasI G'GCGC_C
Cuts at: 0 1667 3609
Size: 1667 1942
MscI TGG'CCA
Cuts at: 0 226 907 3487 3609
Size: 226 681 2580 122
Fragments arranged by size:
2580 681 226 122
MslI CAynn'nnrTG
Cuts at: 0 764 2116 3052 3481 3609
Size: 764 1352 936 429 128
Fragments arranged by size:
1352 936 764 429 128
MspA1I CmG'CkG
Cuts at: 0 660 981 1068 1083 1994 2514 2840 3609
Size: 660 321 87 15 911 520 326 769
Fragments arranged by size:
911 769 660 520 326 321 87 15
NaeI GCC'GGC
Cuts at: 0 2811 3609
Size: 2811 798
NarI GG'CG_CC
Cuts at: 0 1668 3609
Size: 1668 1941
NcoI C'CATG_G
Cuts at: 0 849 3303 3609
Size: 849 2454 306
Fragments arranged by size:
2454 849 306
NgoMIV G'CCGG_C
Cuts at: 0 2809 3609
Size: 2809 800
NspI r_CATG'y
Cuts at: 0 1165 1429 1837 2053 2173 2801 3609
Size: 1165 264 408 216 120 628 808
Fragments arranged by size:
1165 808 628 408 264 216 120
PasI CC'CwG_GG
Cuts at: 0 1263 2229 2604 3129 3609
Size: 1263 966 375 525 480
Fragments arranged by size:
1263 966 525 480 375
PflMI CCAn_nnn'nTGG
Cuts at: 0 1749 1836 3604 3609
Size: 1749 87 1768 5
Fragments arranged by size:
1768 1749 87 5
PfoI T'CCnGG_A
Cuts at: 0 701 1460 3609
Size: 701 759 2149
Fragments arranged by size:
2149 759 701
PmlI CAC'GTG
Cuts at: 0 3105 3609
Size: 3105 504
PpuMI rG'GwC_Cy
Cuts at: 0 676 1929 3609
Size: 676 1253 1680
Fragments arranged by size:
1680 1253 676
PspOMI G'GGCC_C
Cuts at: 0 200 2450 2786 3342 3609
Size: 200 2250 336 556 267
Fragments arranged by size:
2250 556 336 267 200
PstI C_TGCA'G
Cuts at: 0 950 1487 2763 3609
Size: 950 537 1276 846
Fragments arranged by size:
1276 950 846 537
PvuI CG_AT'CG
Cuts at: 0 10 3609
Size: 10 3599
PvuII CAG'CTG
Cuts at: 0 660 981 1083 2514 3609
Size: 660 321 102 1431 1095
Fragments arranged by size:
1431 1095 660 321 102
SacI G_AGCT'C
Cuts at: 0 1904 2564 3609
Size: 1904 660 1045
Fragments arranged by size:
1904 1045 660
SanDI GG'GwC_CC
Cuts at: 0 676 3609
Size: 676 2933
SapI GCTCTTCn'nnn_
Cuts at: 0 136 1909 2090 2569 3609
Size: 136 1773 181 479 1040
Fragments arranged by size:
1773 1040 479 181 136
SexAI A'CCwGG_T
Cuts at: 0 1865 3591 3609
Size: 1865 1726 18
Fragments arranged by size:
1865 1726 18
SfcI C'TryA_G
Cuts at: 0 946 1483 1585 2365 2759 3074 3609
Size: 946 537 102 780 394 315 535
Fragments arranged by size:
946 780 537 535 394 315 102
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 199 1093 3609
Size: 199 894 2516
Fragments arranged by size:
2516 894 199
SfoI GGC'GCC
Cuts at: 0 1669 3609
Size: 1669 1940
SmaI CCC'GGG
Cuts at: 0 2791 3609
Size: 2791 818
SmlI C'TyrA_G
Cuts at: 0 691 2296 3163 3609
Size: 691 1605 867 446
Fragments arranged by size:
1605 867 691 446
SphI G_CATG'C
Cuts at: 0 1165 1429 2173 2801 3609
Size: 1165 264 744 628 808
Fragments arranged by size:
1165 808 744 628 264
SrfI GCCC'GGGC
Cuts at: 0 2791 3609
Size: 2791 818
StuI AGG'CCT
Cuts at: 0 736 1319 3609
Size: 736 583 2290
Fragments arranged by size:
2290 736 583
StyI C'CwwG_G
Cuts at: 0 299 594 849 918 1320 2114 2162 2711
Size: 299 295 255 69 402 794 48 549
Cuts at: 2711 3303 3544 3609
Size: 592 241 65
Fragments arranged by size:
794 592 549 402 299 295 255 241
69 65 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2338 2577 3142 3202 3509 3609
Size: 2338 239 565 60 307 100
Fragments arranged by size:
2338 565 307 239 100 60
TatI w'GTAC_w
Cuts at: 0 3368 3609
Size: 3368 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 56 88 383 415 619 651 1385 1417
Size: 56 32 295 32 204 32 734 32
Cuts at: 1417 3609
Size: 2192
Fragments arranged by size:
2192 734 295 204 56 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3593 3609
Size: 3593 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 76 160 306 1524 1650 2526 2648 3609
Size: 76 84 146 1218 126 876 122 961
Fragments arranged by size:
1218 961 876 146 126 122 84 76
XmaI C'CCGG_G
Cuts at: 0 2789 3609
Size: 2789 820
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApaI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FseI FspI HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuI PvuII SacI SanDI SapI SexAI SfcI SfiI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI AscI AseI AsiSI AvrII BciVI BclI
BfrBI BglII BmgBI BmtI BsaWI BsiWI BsmI BspEI
BspHI BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI
DrdI EcoRI FalI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
RsrII SacII SalI SbfI ScaI SgfI SgrAI SnaBI
SpeI SspI SwaI XbaI XhoI XmnI ZraI