Restriction map for pF1KE0068
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwI        
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BciVI       BfrI        BglI        BlnI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FspI        HpaI        
KasI        KpnI        Ksp632I     KspI        MfeI        
MluI        MseI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccIII       1398  TCCGGA               T^CCGGA
AcyI          982  GGCGTC               G[GA]^CG[CT]C
Alw44I       1270  GTGCAC               G^TGCAC
ApaI         1186  GGGCCC               GGGCC^C
ApaLI        1270  GTGCAC               G^TGCAC
BalI          380  TGGCCA               TGG^CCA
BcgI           62  CGACGCTGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          326  TGATCA               T^GATCA
BfaI          544  CTAG                 C^TAG
BlpI           98  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       98  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1387  TACGTG               [CT]AC^GT[GA]
BsaHI         982  GGCGTC               G[GA]^CG[CT]C
BsaI         1402  GAGACC               GGTCTC(1/5)
BsaWI        1398  TCCGGA               [AT]^CCGG[AT]
BseAI        1398  TCCGGA               T^CCGGA
BsmBI         240  CGTCTC               CGTCTC(1/5)
BspEI        1398  TCCGGA               T^CCGGA
BsrDI         275  CATTGC               GCAATG(2/0)
BssHII        789  GCGCGC               G^CGCGC
BssSI         280  CTCGTG               CACGAG(-5/-1)
BstAPI       1044  GCACCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII          98  GCTCAGC              GC^T[ACGT]AGC
FbaI          326  TGATCA               T^GATCA
FseI          873  GGCCGGCC             GGCCGG^CC
Hin1I         982  GGCGTC               G[GA]^CG[CT]C
HindIII      1462  AAGCTT               A^AGCTT
MaeI          544  CTAG                 C^TAG
MaeII        1388  ACGT                 A^CGT
MaeIII        967  GTAAC                ^GT[ACGT]AC
MluNI         380  TGGCCA               TGG^CCA
MroI         1398  TCCGGA               T^CCGGA
MscI          380  TGGCCA               TGG^CCA
NspI          764  ACATGC               [GA]CATG^[CT]
SmaI          779  CCCGGG               CCC^GGG
XcmI          245  CCATGTTTCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         779  CCCGGG               C^CCGGG
XmaI          779  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AfaI          111  GTAC                 GT^AC
              462  GTAC                
AlwNI         634  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              880  CAGCAGCTG           
BanII         975  GGGCTC               G[GA]GC[CT]^C
             1186  GGGCCC              
BbsI          151  GTCTTC               GAAGAC(2/6)
              538  GTCTTC              
BglII         551  AGATCT               A^GATCT
             1220  AGATCT              
BpuAI         151  GTCTTC               GAAGAC(2/6)
              538  GTCTTC              
BsaBI         999  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1377  GATTTACATC          
BsgI          814  CTGCAC               GTGCAG(16/14)
              431  GTGCAG              
BsmAI        1402  GAGAC                GTCTC(1/5)
              241  GTCTC               
BsrBI         950  CCGCTC               CCGCTC(-3/-3)
             1027  GAGCGG              
BstXI         356  CCATCCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              458  CCATGTACCTGG        
BstYI         551  AGATCT               [GA]^GATC[CT]
             1220  AGATCT              
DraII         316  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1121  GGGTCCC             
EcoO109I      316  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1121  GGGTCCC             
HaeII         448  AGCGCC               [GA]GCGC^[CT]
              606  GGCGCT              
HgaI           63  GACGC                GACGC(5/10)
              983  GCGTC               
HincII        133  GTCAAC               GT[CT]^[GA]AC
              988  GTCAAC              
HindII        133  GTCAAC               GT[CT]^[GA]AC
              988  GTCAAC              
MamI          999  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1377  GATTTACATC          
MflI          551  AGATCT               [GA]^GATC[CT]
             1220  AGATCT              
MspA1I        634  CAGCTG               C[AC]G^C[GT]G
              883  CAGCTG              
NaeI          874  GCCGGC               GCC^GGC
             1057  GCCGGC              
NgoMIV        874  GCCGGC               G^CCGGC
             1057  GCCGGC              
PpuMI         316  GGGACCC              [GA]G^G[AT]CC[CT]
             1121  GGGTCCC             
PstI          631  CTGCAG               CTGCA^G
             1228  CTGCAG              
PvuII         634  CAGCTG               CAG^CTG
              883  CAGCTG              
RsaI          111  GTAC                 GT^AC
              462  GTAC                
TfiI          681  GATTC                G^A[AT]TC
             1358  GAATC               
XhoII         551  AGATCT               [GA]^GATC[CT]
             1220  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaI        
AvaII       BanI        BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       EaeI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MboI        MboII       MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
TspRI       
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