Restriction map for
pF1KE0068
(Circular) (Six-base) MAPSORT of: pF1KE0068.seq Check: 7608 from: 1 to: 4649
pF1KE0068 4649 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1687 1687
Size: 4649
Acc65I G'GTAC_C
Cuts at: 1665 1665
Size: 4649
AclI AA'CG_TT
Cuts at: 4489 4489
Size: 4649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 2474 2906 3653 3921 648
Size: 1826 432 747 268 1376
Fragments arranged by size:
1826 1376 747 432 268
AflIII A'CryG_T
Cuts at: 2001 3105 4082 2001
Size: 1104 977 2568
Fragments arranged by size:
2568 1104 977
AgeI A'CCGG_T
Cuts at: 3037 3037
Size: 4649
AleI CACnn'nnGTG
Cuts at: 724 929 724
Size: 205 4444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 947 947
Size: 4649
AlwNI CAG_nnn'CTG
Cuts at: 747 993 1954 3521 747
Size: 246 961 1567 1875
Fragments arranged by size:
1875 1567 961 246
ApaI G_GGCC'C
Cuts at: 1298 1298
Size: 4649
ApaLI G'TGCA_C
Cuts at: 1378 3419 1378
Size: 2041 2608
ApoI r'AATT_y
Cuts at: 1653 4002 4258 1653
Size: 2349 256 2044
Fragments arranged by size:
2349 2044 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4649
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
AvaI C'yCGr_G
Cuts at: 518 887 1234 1669 518
Size: 369 347 435 3498
Fragments arranged by size:
3498 435 369 347
BamHI G'GATC_C
Cuts at: 1674 1674
Size: 4649
BanI G'GyrC_C
Cuts at: 726 1151 1438 1665 2079 2327 2362 726
Size: 425 287 227 414 248 35 3013
Fragments arranged by size:
3013 425 414 287 248 227 35
BanII G_rGCy'C
Cuts at: 1087 1298 1663 2693 1087
Size: 211 365 1030 3043
Fragments arranged by size:
3043 1030 365 211
BbeI G_GCGC'C
Cuts at: 2083 2331 2083
Size: 248 4401
BbsI GAAGACnn'nnnn_
Cuts at: 252 639 252
Size: 387 4262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 159 193 159
Size: 34 4615
BciVI GTATCCnnnnn_n'
Cuts at: 2547 3308 4649 2547
Size: 761 1341 2547
Fragments arranged by size:
2547 1341 761
BclI T'GATC_A
Cuts at: 434 2169 434
Size: 1735 2914
BglII A'GATC_T
Cuts at: 659 1328 2164 4201 659
Size: 669 836 2037 1107
Fragments arranged by size:
2037 1107 836 669
BlpI GC'TnA_GC
Cuts at: 207 1761 207
Size: 1554 3095
Bme1580I G_kGCm'C
Cuts at: 731 936 1298 1382 2274 2367 3423 731
Size: 205 362 84 892 93 1056 1957
Fragments arranged by size:
1957 1056 892 362 205 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 1292 1861 1942 2277 4421 1292
Size: 569 81 335 2144 1520
Fragments arranged by size:
2144 1520 569 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 655 886 148
Size: 507 231 3911
Fragments arranged by size:
3911 507 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2704 3196 3494 1825
Size: 879 492 298 2980
Fragments arranged by size:
2980 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1504 4353 1504
Size: 2849 1800
BsaAI yAC'GTr
Cuts at: 1497 2002 1497
Size: 505 4144
BsaBI GATnn'nnATC
Cuts at: 6 1111 1489 2163 4200 6
Size: 1105 378 674 2037 455
Fragments arranged by size:
2037 1105 674 455 378
BsaHI Gr'CG_yC
Cuts at: 1091 2080 2328 3030 4526 1091
Size: 989 248 702 1496 1214
Fragments arranged by size:
1496 1214 989 702 248
BsaWI w'CCGG_w
Cuts at: 1506 1838 2359 3037 3311 3458 3869 1506
Size: 332 521 678 274 147 411 2286
Fragments arranged by size:
2286 678 521 411 332 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1605 1635 1605
Size: 30 4619
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 843 949 1465 1594 843
Size: 106 516 129 3898
Fragments arranged by size:
3898 516 129 106
BseYI C'CCAG_C
Cuts at: 528 1140 1603 2074 2832 3409 3905 528
Size: 612 463 471 758 577 496 1272
Fragments arranged by size:
1272 758 612 577 496 471 463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 907 560
Size: 347 4302
BsiEI CG_ry'CG
Cuts at: 104 2191 2237 3445 104
Size: 2087 46 1208 1308
Fragments arranged by size:
2087 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 977 1214 1382 1663 2441 2631 3423 977
Size: 237 168 281 778 190 792 2203
Fragments arranged by size:
2203 792 778 281 237 190 168
BsmBI CGTCTCn'nnnn_
Cuts at: 354 354
Size: 4649
Bsp1286I G_dGCh'C
Cuts at: 731 936 977 1087 1214 1298 1382 1663 2274
Size: 205 41 110 127 84 84 281 611
Cuts at: 2274 2367 2441 2631 2693 3423 731
Size: 93 74 190 62 730 1957
Fragments arranged by size:
1957 730 611 281 205 190 127 110
93 84 84 74 62 41
BspEI T'CCGG_A
Cuts at: 1506 3869 1506
Size: 2363 2286
BspHI T'CATG_A
Cuts at: 3825 3825
Size: 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1699 1913 2215 2596 1699
Size: 214 302 381 3752
Fragments arranged by size:
3752 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1060 1137 2941 2995 4479 4644 50
Size: 1010 77 1804 54 1484 165 55
Fragments arranged by size:
1804 1484 1010 165 77 55 54
BsrDI GCAATG_nn'
Cuts at: 382 2561 382
Size: 2179 2470
BsrFI r'CCGG_y
Cuts at: 702 982 1165 2647 2828 3037 702
Size: 280 183 1482 181 209 2314
Fragments arranged by size:
2314 1482 280 209 183 181
BssHII G'CGCG_C
Cuts at: 897 897
Size: 4649
BssSI C'ACGA_G
Cuts at: 388 2920 3278 388
Size: 2532 358 1759
Fragments arranged by size:
2532 1759 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1158 2026 1158
Size: 868 3781
BstBI TT'CG_AA
Cuts at: 3010 3010
Size: 4649
BstXI CCAn_nnnn'nTGG
Cuts at: 471 573 471
Size: 102 4547
BstYI r'GATC_y
Cuts at: 1 659 1328 1674 2141 2164 2499 2745 3757
Size: 658 669 346 467 23 335 246 1012
Cuts at: 3757 3843 3855 4201 1
Size: 86 12 346 449
Fragments arranged by size:
1012 669 658 467 449 346 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4649
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1260 2599 2780 2943 1260
Size: 1339 181 163 2966
Fragments arranged by size:
2966 1339 181 163
BtsI GCAGTG_nn'
Cuts at: 1382 1478 1892 1382
Size: 96 414 4139
Fragments arranged by size:
4139 414 96
DraI TTT'AAA
Cuts at: 1648 1648
Size: 4649
DrdI GACnn_nn'nnGTC
Cuts at: 2355 3213 2355
Size: 858 3791
EaeI y'GGCC_r
Cuts at: 488 700 980 984 1163 2234 2408 2799 2826
Size: 212 280 4 179 1071 174 391 27
Cuts at: 2826 4171 488
Size: 1345 966
Fragments arranged by size:
1345 1071 966 391 280 212 179 174
27 4
EagI C'GGCC_G
Cuts at: 2234 2234
Size: 4649
EarI CTCTTCn'nnn_
Cuts at: 2672 2882 2672
Size: 210 4439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2857 3167 3313 4459 2857
Size: 310 146 1146 3047
Fragments arranged by size:
3047 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1661 1661
Size: 4649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 648 655 886 2474 2906 3653 3921 148
Size: 500 7 231 1588 432 747 268 876
Fragments arranged by size:
1588 876 747 500 432 268 231 7
EcoO109I rG'GnC_Cy
Cuts at: 425 1230 1788 425
Size: 805 558 3286
Fragments arranged by size:
3286 805 558
EcoRI G'AATT_C
Cuts at: 1653 1653
Size: 4649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4649
FseI GG_CCGG'CC
Cuts at: 986 986
Size: 4649
FspI TGC'GCA
Cuts at: 2430 2430
Size: 4649
HaeII r_GCGC'y
Cuts at: 560 718 2083 2331 3353 4332 560
Size: 158 1365 248 1022 979 877
Fragments arranged by size:
1365 1022 979 877 248 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2491 2521 2523 2553 2491
Size: 30 2 30 4587
Fragments arranged by size:
4587 30 30 2
HincII GTy'rAC
Cuts at: 243 1098 1688 243
Size: 855 590 3204
Fragments arranged by size:
3204 855 590
HindIII A'AGCT_T
Cuts at: 1570 1570
Size: 4649
KasI G'GCGC_C
Cuts at: 2079 2327 2079
Size: 248 4401
KpnI G_GTAC'C
Cuts at: 1669 1669
Size: 4649
MluI A'CGCG_T
Cuts at: 4082 4082
Size: 4649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 621 3320 3504 621
Size: 2699 184 1766
Fragments arranged by size:
2699 1766 184
MscI TGG'CCA
Cuts at: 490 2410 490
Size: 1920 2729
MslI CAynn'nnrTG
Cuts at: 469 724 850 929 970 1105 1495 2638 2765
Size: 255 126 79 41 135 390 1143 127
Cuts at: 2765 469
Size: 2353
Fragments arranged by size:
2353 1143 390 255 135 127 126 79
41
MspA1I CmG'CkG
Cuts at: 744 993 1760 2074 2434 3447 3692 744
Size: 249 767 314 360 1013 245 1701
Fragments arranged by size:
1701 1013 767 360 314 249 245
NaeI GCC'GGC
Cuts at: 984 1167 2830 984
Size: 183 1663 2803
Fragments arranged by size:
2803 1663 183
NarI GG'CG_CC
Cuts at: 2080 2328 2080
Size: 248 4401
NcoI C'CATG_G
Cuts at: 107 107
Size: 4649
NgoMIV G'CCGG_C
Cuts at: 982 1165 2828 982
Size: 183 1663 2803
Fragments arranged by size:
2803 1663 183
NspI r_CATG'y
Cuts at: 876 1702 2202 3109 876
Size: 826 500 907 2416
Fragments arranged by size:
2416 907 826 500
PciI A'CATG_T
Cuts at: 3105 3105
Size: 4649
PfoI T'CCnGG_A
Cuts at: 1424 1424
Size: 4649
PmeI GTTT'AAAC
Cuts at: 1648 1648
Size: 4649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4617
PpuMI rG'GwC_Cy
Cuts at: 425 1230 425
Size: 805 3844
PspOMI G'GGCC_C
Cuts at: 1294 1294
Size: 4649
PstI C_TGCA'G
Cuts at: 743 1340 1696 2381 743
Size: 597 356 685 3011
Fragments arranged by size:
3011 685 597 356
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4649
PvuII CAG'CTG
Cuts at: 744 993 2074 2434 744
Size: 249 1081 360 2959
Fragments arranged by size:
2959 1081 360 249
RsrII CG'GwC_CG
Cuts at: 2844 2844
Size: 4649
SacI G_AGCT'C
Cuts at: 1663 1663
Size: 4649
SalI G'TCGA_C
Cuts at: 1686 1686
Size: 4649
SanDI GG'GwC_CC
Cuts at: 425 1230 425
Size: 805 3844
SapI GCTCTTCn'nnn_
Cuts at: 2672 2882 2672
Size: 210 4439
SbfI CC_TGCA'GG
Cuts at: 1696 1696
Size: 4649
ScaI AGT'ACT
Cuts at: 4166 4166
Size: 4649
SfcI C'TryA_G
Cuts at: 33 552 739 1336 1443 1692 2377 3370 3561
Size: 519 187 597 107 249 685 993 191
Cuts at: 3561 33
Size: 1121
Fragments arranged by size:
1121 993 685 597 519 249 191 187
107
SfoI GGC'GCC
Cuts at: 2081 2329 2081
Size: 248 4401
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
SmaI CCC'GGG
Cuts at: 889 1671 889
Size: 782 3867
SmlI C'TyrA_G
Cuts at: 1804 2719 3211 3473 1804
Size: 915 492 262 2980
Fragments arranged by size:
2980 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4649
SphI G_CATG'C
Cuts at: 1702 2202 1702
Size: 500 4149
SrfI GCCC'GGGC
Cuts at: 889 889
Size: 4649
StyI C'CwwG_G
Cuts at: 107 491 1514 1628 1783 2137 107
Size: 384 1023 114 155 354 2619
Fragments arranged by size:
2619 1023 384 354 155 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 637 1339 2232 2365 2824 3033 153
Size: 484 702 893 133 459 209 1769
Fragments arranged by size:
1769 893 702 484 459 209 133
TatI w'GTAC_w
Cuts at: 4164 4164
Size: 4649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1605 1637 4166 4198 1605
Size: 32 2529 32 2056
Fragments arranged by size:
2529 2056 32 32
Tth111I GACn'n_nGTC
Cuts at: 2446 2446
Size: 4649
XbaI T'CTAG_A
Cuts at: 1680 1680
Size: 4649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 360 2144 360
Size: 1784 2865
XmaI C'CCGG_G
Cuts at: 887 1669 887
Size: 782 3867
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI