Restriction map for pF1KE0068
 (Linear) (Six-base) MAPSORT of: KIEE0068.seq  Check: 9094  from: 1  to: 1536



KIEE0068.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     540    1536 
   Size:        540     996
 
AleI CACnn'nnGTG

Cuts at:      0     616     821    1536 
   Size:        616     205     715

  Fragments arranged by size: 

                 715     616     205
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     839    1536 
   Size:        839     697
 
AlwNI CAG_nnn'CTG

Cuts at:      0     639     885    1536 
   Size:        639     246     651

  Fragments arranged by size: 

                 651     639     246
 
ApaI G_GGCC'C

Cuts at:      0    1190    1536 
   Size:       1190     346
 
ApaLI G'TGCA_C

Cuts at:      0    1270    1536 
   Size:       1270     266
 
AvaI C'yCGr_G

Cuts at:      0     410     779    1126    1536 
   Size:        410     369     347     410

  Fragments arranged by size: 

                 410     410     369     347
 
BanI G'GyrC_C

Cuts at:      0     618    1043    1330    1536 
   Size:        618     425     287     206

  Fragments arranged by size: 

                 618     425     287     206
 
BanII G_rGCy'C

Cuts at:      0     979    1190    1536 
   Size:        979     211     346

  Fragments arranged by size: 

                 979     346     211
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     144     531    1536 
   Size:        144     387    1005

  Fragments arranged by size: 

                1005     387     144
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      51      85    1536 
   Size:         51      34    1451

  Fragments arranged by size: 

                1451      51      34
 
BclI T'GATC_A

Cuts at:      0     326    1536 
   Size:        326    1210
 
BglII A'GATC_T

Cuts at:      0     551    1220    1536 
   Size:        551     669     316

  Fragments arranged by size: 

                 669     551     316
 
BlpI GC'TnA_GC

Cuts at:      0      99    1536 
   Size:         99    1437
 
Bme1580I G_kGCm'C

Cuts at:      0     623     828    1190    1274    1536 
   Size:        623     205     362      84     262

  Fragments arranged by size: 

                 623     362     262     205      84
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1184    1536 
   Size:       1184     352
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     547     778    1536 
   Size:         40     507     231     758

  Fragments arranged by size: 

                 758     507     231      40
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1396    1536 
   Size:       1396     140
 
BsaAI yAC'GTr

Cuts at:      0    1389    1536 
   Size:       1389     147
 
BsaBI GATnn'nnATC

Cuts at:      0    1003    1381    1536 
   Size:       1003     378     155

  Fragments arranged by size: 

                1003     378     155
 
BsaHI Gr'CG_yC

Cuts at:      0     983    1536 
   Size:        983     553
 
BsaWI w'CCGG_w

Cuts at:      0    1398    1536 
   Size:       1398     138
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1497    1527    1536 
   Size:       1497      30       9

  Fragments arranged by size: 

                1497      30       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     735     841    1357    1486    1536 
   Size:        735     106     516     129      50

  Fragments arranged by size: 

                 735     516     129     106      50
 
BseYI C'CCAG_C

Cuts at:      0     420    1032    1495    1536 
   Size:        420     612     463      41

  Fragments arranged by size: 

                 612     463     420      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     799    1536 
   Size:        452     347     737

  Fragments arranged by size: 

                 737     452     347
 
BsiHKAI G_wGCw'C

Cuts at:      0     869    1106    1274    1536 
   Size:        869     237     168     262

  Fragments arranged by size: 

                 869     262     237     168
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     246    1536 
   Size:        246    1290
 
Bsp1286I G_dGCh'C

Cuts at:      0     623     828     869     979    1106    1190    1274    1536 
   Size:        623     205      41     110     127      84      84     262

  Fragments arranged by size: 

                 623     262     205     127     110      84      84      41
            
 
BspEI T'CCGG_A

Cuts at:      0    1398    1536 
   Size:       1398     138
 
BsrBI CCG'CTC

Cuts at:      0     952    1029    1536 
   Size:        952      77     507

  Fragments arranged by size: 

                 952     507      77
 
BsrDI GCAATG_nn'

Cuts at:      0     274    1536 
   Size:        274    1262
 
BsrFI r'CCGG_y

Cuts at:      0     594     874    1057    1536 
   Size:        594     280     183     479

  Fragments arranged by size: 

                 594     479     280     183
 
BssHII G'CGCG_C

Cuts at:      0     789    1536 
   Size:        789     747
 
BssSI C'ACGA_G

Cuts at:      0     280    1536 
   Size:        280    1256
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1050    1536 
   Size:       1050     486
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     363     465    1536 
   Size:        363     102    1071

  Fragments arranged by size: 

                1071     363     102
 
BstYI r'GATC_y

Cuts at:      0     551    1220    1536 
   Size:        551     669     316

  Fragments arranged by size: 

                 669     551     316
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1152    1536 
   Size:       1152     384
 
BtsI GCAGTG_nn'

Cuts at:      0    1274    1370    1536 
   Size:       1274      96     166

  Fragments arranged by size: 

                1274     166      96
 
EaeI y'GGCC_r

Cuts at:      0     380     592     872     876    1055    1536 
   Size:        380     212     280       4     179     481

  Fragments arranged by size: 

                 481     380     280     212     179       4
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     540     547     778    1536 
   Size:         40     500       7     231     758

  Fragments arranged by size: 

                 758     500     231      40       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     317    1122    1536 
   Size:        317     805     414

  Fragments arranged by size: 

                 805     414     317
 
FseI GG_CCGG'CC

Cuts at:      0     878    1536 
   Size:        878     658
 
HaeII r_GCGC'y

Cuts at:      0     452     610    1536 
   Size:        452     158     926

  Fragments arranged by size: 

                 926     452     158
 
HincII GTy'rAC

Cuts at:      0     135     990    1536 
   Size:        135     855     546

  Fragments arranged by size: 

                 855     546     135
 
HindIII A'AGCT_T

Cuts at:      0    1462    1536 
   Size:       1462      74
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     513    1536 
   Size:        513    1023
 
MscI TGG'CCA

Cuts at:      0     382    1536 
   Size:        382    1154
 
MslI CAynn'nnrTG

Cuts at:      0     361     616     742     821     862     997    1387    1536 
   Size:        361     255     126      79      41     135     390     149

  Fragments arranged by size: 

                 390     361     255     149     135     126      79      41
            
 
MspA1I CmG'CkG

Cuts at:      0     636     885    1536 
   Size:        636     249     651

  Fragments arranged by size: 

                 651     636     249
 
NaeI GCC'GGC

Cuts at:      0     876    1059    1536 
   Size:        876     183     477

  Fragments arranged by size: 

                 876     477     183
 
NgoMIV G'CCGG_C

Cuts at:      0     874    1057    1536 
   Size:        874     183     479

  Fragments arranged by size: 

                 874     479     183
 
NspI r_CATG'y

Cuts at:      0     768    1536 
   Size:        768     768
 
PfoI T'CCnGG_A

Cuts at:      0    1316    1536 
   Size:       1316     220
 
PpuMI rG'GwC_Cy

Cuts at:      0     317    1122    1536 
   Size:        317     805     414

  Fragments arranged by size: 

                 805     414     317
 
PspOMI G'GGCC_C

Cuts at:      0    1186    1536 
   Size:       1186     350
 
PstI C_TGCA'G

Cuts at:      0     635    1232    1536 
   Size:        635     597     304

  Fragments arranged by size: 

                 635     597     304
 
PvuII CAG'CTG

Cuts at:      0     636     885    1536 
   Size:        636     249     651

  Fragments arranged by size: 

                 651     636     249
 
SanDI GG'GwC_CC

Cuts at:      0     317    1122    1536 
   Size:        317     805     414

  Fragments arranged by size: 

                 805     414     317
 
SfcI C'TryA_G

Cuts at:      0     444     631    1228    1335    1536 
   Size:        444     187     597     107     201

  Fragments arranged by size: 

                 597     444     201     187     107
 
SmaI CCC'GGG

Cuts at:      0     781    1536 
   Size:        781     755
 
SrfI GCCC'GGGC

Cuts at:      0     781    1536 
   Size:        781     755
 
StyI C'CwwG_G

Cuts at:      0     383    1406    1520    1536 
   Size:        383    1023     114      16

  Fragments arranged by size: 

                1023     383     114      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      45     529    1231    1536 
   Size:         45     484     702     305

  Fragments arranged by size: 

                 702     484     305      45
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1497    1529    1536 
   Size:       1497      32       7

  Fragments arranged by size: 

                1497      32       7
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     252    1536 
   Size:        252    1284
 
XmaI C'CCGG_G

Cuts at:      0     779    1536 
   Size:        779     757
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI
    BanII     BbsI     BcgI     BclI    BglII     BlpI Bme1580I     BmrI
     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI    BtgZI     BtsI
     EaeI  Eco57MI EcoO109I     FseI    HaeII   HincII  HindIII     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PfoI    PpuMI
   PspOMI     PstI    PvuII    SanDI     SfcI     SmaI     SrfI     StyI
    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI    BfrBI     BglI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsiEI    BsiWI     BsmI    BspHI
    BspMI    BsrGI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FspI    FspAI    Hin4I     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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