Restriction map for pF1KE0066
 (Linear) (Six-base) MAPSORT of: KIEE0066.seq  Check: 7417  from: 1  to: 2679



KIEE0066.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:      0     635     677     692    2679 
   Size:        635      42      15    1987

  Fragments arranged by size: 

                1987     635      42      15
 
Acc65I G'GTAC_C

Cuts at:      0     808    2679 
   Size:        808    1871
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     703    1090    1783    2387    2627    2679 
   Size:        306     397     387     693     604     240      52

  Fragments arranged by size: 

                 693     604     397     387     306     240      52
 
AfeI AGC'GCT

Cuts at:      0    1816    2679 
   Size:       1816     863
 
AflII C'TTAA_G

Cuts at:      0    1126    2679 
   Size:       1126    1553
 
AflIII A'CryG_T

Cuts at:      0     527    2439    2441    2679 
   Size:        527    1912       2     238

  Fragments arranged by size: 

                1912     527     238       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     490    2002    2103    2220    2679 
   Size:        490    1512     101     117     459

  Fragments arranged by size: 

                1512     490     459     117     101
 
ApaI G_GGCC'C

Cuts at:      0     169    2679 
   Size:        169    2510
 
AvaI C'yCGr_G

Cuts at:      0      14      82     155     217     244    1833    2679 
   Size:         14      68      73      62      27    1589     846

  Fragments arranged by size: 

                1589     846      73      68      62      27      14
 
BanI G'GyrC_C

Cuts at:      0     151     178     204     808    2679 
   Size:        151      27      26     604    1871

  Fragments arranged by size: 

                1871     604     151      27      26
 
BanII G_rGCy'C

Cuts at:      0     169     317    1289    2576    2679 
   Size:        169     148     972    1287     103

  Fragments arranged by size: 

                1287     972     169     148     103
 
BbeI G_GCGC'C

Cuts at:      0     155     182     208    2679 
   Size:        155      27      26    2471

  Fragments arranged by size: 

                2471     155      27      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     425    1304    2375    2679 
   Size:        425     879    1071     304

  Fragments arranged by size: 

                1071     879     425     304
 
BbvCI CC'TCA_GC

Cuts at:      0    1774    2233    2679 
   Size:       1774     459     446

  Fragments arranged by size: 

                1774     459     446
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     805    1073    2679 
   Size:        805     268    1606

  Fragments arranged by size: 

                1606     805     268
 
BclI T'GATC_A

Cuts at:      0    2472    2679 
   Size:       2472     207
 
BglI GCCn_nnn'nGGC

Cuts at:      0     121     187    2006    2679 
   Size:        121      66    1819     673

  Fragments arranged by size: 

                1819     673     121      66
 
BlpI GC'TnA_GC

Cuts at:      0      74     229     316    2679 
   Size:         74     155      87    2363

  Fragments arranged by size: 

                2363     155      87      74
 
Bme1580I G_kGCm'C

Cuts at:      0     169    1619    2679 
   Size:        169    1450    1060

  Fragments arranged by size: 

                1450    1060     169
 
BmgBI CAC'GTC

Cuts at:      0     639    2679 
   Size:        639    2040
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2284    2625    2679 
   Size:       2284     341      54

  Fragments arranged by size: 

                2284     341      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1444    1889    1979    2112    2679 
   Size:       1444     445      90     133     567

  Fragments arranged by size: 

                1444     567     445     133      90
 
Bpu10I CC'TnA_GC

Cuts at:      0     610    1097    1774    2233    2679 
   Size:        610     487     677     459     446

  Fragments arranged by size: 

                 677     610     487     459     446
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1437    1910    2679 
   Size:        364    1073     473     769

  Fragments arranged by size: 

                1073     769     473     364
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1997    2679 
   Size:       1997     682
 
BsaAI yAC'GTr

Cuts at:      0    2442    2679 
   Size:       2442     237
 
BsaHI Gr'CG_yC

Cuts at:      0       4     152     179     205    2679 
   Size:          4     148      27      26    2474

  Fragments arranged by size: 

                2474     148      27      26       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     325     439    2115    2529    2679 
   Size:        325     114    1676     414     150

  Fragments arranged by size: 

                1676     414     325     150     114
 
BseYI C'CCAG_C

Cuts at:      0     101     190     300     326    2075    2176    2679 
   Size:        101      89     110      26    1749     101     503

  Fragments arranged by size: 

                1749     503     110     101     101      89      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2089    2679 
   Size:       2089     590
 
BsiEI CG_ry'CG

Cuts at:      0     116    2679 
   Size:        116    2563
 
BsiHKAI G_wGCw'C

Cuts at:      0     100    1289    1880    2679 
   Size:        100    1189     591     799

  Fragments arranged by size: 

                1189     799     591     100
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1724    2679 
   Size:       1724     955
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     169     317    1289    1619    1880    2576    2679 
   Size:        100      69     148     972     330     261     696     103

  Fragments arranged by size: 

                 972     696     330     261     148     103     100      69
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     470    2679 
   Size:        470    2209
 
BsrBI CCG'CTC

Cuts at:      0     149    2679 
   Size:        149    2530
 
BsrDI GCAATG_nn'

Cuts at:      0    1522    2354    2679 
   Size:       1522     832     325

  Fragments arranged by size: 

                1522     832     325
 
BsrFI r'CCGG_y

Cuts at:      0     181    2089    2679 
   Size:        181    1908     590

  Fragments arranged by size: 

                1908     590     181
 
BssHII G'CGCG_C

Cuts at:      0     401     450    2679 
   Size:        401      49    2229

  Fragments arranged by size: 

                2229     401      49
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     805    2136    2679 
   Size:        805    1331     543

  Fragments arranged by size: 

                1331     805     543
 
BstYI r'GATC_y

Cuts at:      0    1101    2282    2679 
   Size:       1101    1181     397

  Fragments arranged by size: 

                1181    1101     397
 
BstZ17I GTA'TAC

Cuts at:      0     678    2679 
   Size:        678    2001
 
Bsu36I CC'TnA_GG

Cuts at:      0     655     880    2084    2096    2666    2679 
   Size:        655     225    1204      12     570      13

  Fragments arranged by size: 

                1204     655     570     225      13      12
 
BtgI C'CryG_G

Cuts at:      0     372    2679 
   Size:        372    2307
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     157    2166    2190    2679 
   Size:        157    2009      24     489

  Fragments arranged by size: 

                2009     489     157      24
 
BtsI GCAGTG_nn'

Cuts at:      0     132    2052    2679 
   Size:        132    1920     627

  Fragments arranged by size: 

                1920     627     132
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9    2386    2679 
   Size:          9    2377     293

  Fragments arranged by size: 

                2377     293       9
 
EaeI y'GGCC_r

Cuts at:      0     113    2679 
   Size:        113    2566
 
EagI C'GGCC_G

Cuts at:      0     113    2679 
   Size:        113    2566
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      34      51    2679 
   Size:         34      17    2628

  Fragments arranged by size: 

                2628      34      17
 
EcoICRI GAG'CTC

Cuts at:      0    1287    2679 
   Size:       1287    1392
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     703    1090    1444    1783    1889    1979    2112 
   Size:        306     397     387     354     339     106      90     133

Cuts at:   2112    2387    2627    2679 
   Size:        275     240      52

  Fragments arranged by size: 

                 397     387     354     339     306     275     240     133
                 106      90      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1462    2679 
   Size:       1462    1217
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165     384    1710    1767    1999    2679 
   Size:        165     219    1326      57     232     680

  Fragments arranged by size: 

                1326     680     232     219     165      57
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1332    1431    2593    2679 
   Size:       1332      99    1162      86

  Fragments arranged by size: 

                1332    1162      99      86
 
FseI GG_CCGG'CC

Cuts at:      0    2093    2679 
   Size:       2093     586
 
HaeII r_GCGC'y

Cuts at:      0      63     155     182     208     412    1365    1707    1818 
   Size:         63      92      27      26     204     953     342     111

Cuts at:   1818    2679 
   Size:        861

  Fragments arranged by size: 

                 953     861     342     204     111      92      63      27
                  26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     964     996    2649    2679 
   Size:        964      32    1653      30

  Fragments arranged by size: 

                1653     964      32      30
 
HincII GTy'rAC

Cuts at:      0     636    2679 
   Size:        636    2043
 
HindIII A'AGCT_T

Cuts at:      0    2320    2478    2679 
   Size:       2320     158     201

  Fragments arranged by size: 

                2320     201     158
 
KasI G'GCGC_C

Cuts at:      0     151     178     204    2679 
   Size:        151      27      26    2475

  Fragments arranged by size: 

                2475     151      27      26
 
KpnI G_GTAC'C

Cuts at:      0     812    2679 
   Size:        812    1867
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     837    2679 
   Size:        837    1842
 
MslI CAynn'nnrTG

Cuts at:      0     817    1438    1804    1855    2679 
   Size:        817     621     366      51     824

  Fragments arranged by size: 

                 824     817     621     366      51
 
MspA1I CmG'CkG

Cuts at:      0     261     326     374    1782    2241    2289    2679 
   Size:        261      65      48    1408     459      48     390

  Fragments arranged by size: 

                1408     459     390     261      65      48      48
 
NaeI GCC'GGC

Cuts at:      0     183    2091    2679 
   Size:        183    1908     588

  Fragments arranged by size: 

                1908     588     183
 
NarI GG'CG_CC

Cuts at:      0     152     179     205    2679 
   Size:        152      27      26    2474

  Fragments arranged by size: 

                2474     152      27      26
 
NdeI CA'TA_TG

Cuts at:      0    1982    2679 
   Size:       1982     697
 
NgoMIV G'CCGG_C

Cuts at:      0     181    2089    2679 
   Size:        181    1908     590

  Fragments arranged by size: 

                1908     590     181
 
NspI r_CATG'y

Cuts at:      0     531    2341    2397    2679 
   Size:        531    1810      56     282

  Fragments arranged by size: 

                1810     531     282      56
 
PasI CC'CwG_GG

Cuts at:      0    1752    2259    2679 
   Size:       1752     507     420

  Fragments arranged by size: 

                1752     507     420
 
PciI A'CATG_T

Cuts at:      0     527    2679 
   Size:        527    2152
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2002    2038    2624    2679 
   Size:       2002      36     586      55

  Fragments arranged by size: 

                2002     586      55      36
 
PfoI T'CCnGG_A

Cuts at:      0     824    1263    2679 
   Size:        824     439    1416

  Fragments arranged by size: 

                1416     824     439
 
PmlI CAC'GTG

Cuts at:      0    2442    2679 
   Size:       2442     237
 
PpuMI rG'GwC_Cy

Cuts at:      0    1710    1767    2679 
   Size:       1710      57     912

  Fragments arranged by size: 

                1710     912      57
 
PspOMI G'GGCC_C

Cuts at:      0     165    2679 
   Size:        165    2514
 
PstI C_TGCA'G

Cuts at:      0    2347    2679 
   Size:       2347     332
 
PvuII CAG'CTG

Cuts at:      0     261     326    1782    2241    2289    2679 
   Size:        261      65    1456     459      48     390

  Fragments arranged by size: 

                1456     459     390     261      65      48
 
SacI G_AGCT'C

Cuts at:      0    1289    2679 
   Size:       1289    1390
 
SacII CC_GC'GG

Cuts at:      0     375    2679 
   Size:        375    2304
 
SalI G'TCGA_C

Cuts at:      0     634    2679 
   Size:        634    2045
 
SfcI C'TryA_G

Cuts at:      0     575    2343    2679 
   Size:        575    1768     336

  Fragments arranged by size: 

                1768     575     336
 
SfoI GGC'GCC

Cuts at:      0     153     180     206    2679 
   Size:        153      27      26    2473

  Fragments arranged by size: 

                2473     153      27      26
 
SgrAI Cr'CCGG_yG

Cuts at:      0     181    2679 
   Size:        181    2498
 
SmaI CCC'GGG

Cuts at:      0      16      84     157     219     246    2679 
   Size:         16      68      73      62      27    2433

  Fragments arranged by size: 

                2433      73      68      62      27      16
 
SmlI C'TyrA_G

Cuts at:      0     379    1126    1416    1925    2679 
   Size:        379     747     290     509     754

  Fragments arranged by size: 

                 754     747     509     379     290
 
SphI G_CATG'C

Cuts at:      0    2341    2679 
   Size:       2341     338
 
SrfI GCCC'GGGC

Cuts at:      0     246    2679 
   Size:        246    2433
 
StuI AGG'CCT

Cuts at:      0    1902    2664    2679 
   Size:       1902     762      15

  Fragments arranged by size: 

                1902     762      15
 
StyI C'CwwG_G

Cuts at:      0    1400    2577    2679 
   Size:       1400    1177     102

  Fragments arranged by size: 

                1400    1177     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     115     619    2227    2679 
   Size:        115     504    1608     452

  Fragments arranged by size: 

                1608     504     452     115
 
TatI w'GTAC_w

Cuts at:      0    1535    2679 
   Size:       1535    1144
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     651     683    1732    1764    2679 
   Size:        651      32    1049      32     915

  Fragments arranged by size: 

                1049     915     651      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1504    1609    2679 
   Size:       1504     105    1070

  Fragments arranged by size: 

                1504    1070     105
 
XmaI C'CCGG_G

Cuts at:      0      14      82     155     217     244    2679 
   Size:         14      68      73      62      27    2435

  Fragments arranged by size: 

                2435      73      68      62      27      14
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI    AflII   AflIII    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FseI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII     SalI     SfcI     SfoI    SgrAI     SmaI     SmlI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AgeI     AhdI     AleI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BfrBI    BglII     BmtI     BplI    BsaBI    BsaWI    BsaXI
    BsiWI     BsmI    BspEI    BspHI    BsrGI    BssSI   BstAPI    BstBI
   BstEII     ClaI    CspCI     DraI   DraIII     EarI    EcoRI    EcoRV
     FspI    FspAI     HpaI     MfeI     MluI     MscI     NcoI     NheI
     NotI     NruI     NsiI     PacI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SnaBI     SpeI     SspI     SwaI  Tth111I
     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com