Restriction map for pF1KE0064
 (Linear) (Six-base) MAPSORT of: KIEE0064.seq  Check: 1477  from: 1  to: 1737



KIEE0064.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     228    1737 
   Size:        228    1509
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     884    1737 
   Size:        884     853
 
AflIII A'CryG_T

Cuts at:      0    1116    1737 
   Size:       1116     621
 
AleI CACnn'nnGTG

Cuts at:      0     211     277    1023    1737 
   Size:        211      66     746     714

  Fragments arranged by size: 

                 746     714     211      66
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     862    1737 
   Size:        862     875
 
AlwNI CAG_nnn'CTG

Cuts at:      0     647     923    1737 
   Size:        647     276     814

  Fragments arranged by size: 

                 814     647     276
 
ApaI G_GGCC'C

Cuts at:      0    1510    1737 
   Size:       1510     227
 
AvrII C'CTAG_G

Cuts at:      0    1536    1737 
   Size:       1536     201
 
BanI G'GyrC_C

Cuts at:      0    1090    1446    1737 
   Size:       1090     356     291

  Fragments arranged by size: 

                1090     356     291
 
BanII G_rGCy'C

Cuts at:      0     715     830    1469    1510    1737 
   Size:        715     115     639      41     227

  Fragments arranged by size: 

                 715     639     227     115      41
 
BbeI G_GCGC'C

Cuts at:      0    1094    1450    1737 
   Size:       1094     356     287

  Fragments arranged by size: 

                1094     356     287
 
BbvCI CC'TCA_GC

Cuts at:      0     476    1108    1737 
   Size:        476     632     629

  Fragments arranged by size: 

                 632     629     476
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     727     761    1737 
   Size:        727      34     976

  Fragments arranged by size: 

                 976     727      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1307    1737 
   Size:       1307     430
 
BfrBI ATG'CAT

Cuts at:      0    1238    1737 
   Size:       1238     499
 
Bme1580I G_kGCm'C

Cuts at:      0     695    1510    1737 
   Size:        695     815     227

  Fragments arranged by size: 

                 815     695     227
 
BmgBI CAC'GTC

Cuts at:      0     961    1129    1213    1219    1381    1737 
   Size:        961     168      84       6     162     356

  Fragments arranged by size: 

                 961     356     168     162      84       6
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     151     439     534     573     612     690    1737 
   Size:        151     288      95      39      39      78    1047

  Fragments arranged by size: 

                1047     288     151      95      78      39      39
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     849    1005    1737 
   Size:        849     156     732

  Fragments arranged by size: 

                 849     732     156
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     819    1724    1737 
   Size:        392     427     905      13

  Fragments arranged by size: 

                 905     427     392      13
 
Bpu10I CC'TnA_GC

Cuts at:      0     476    1103    1108    1737 
   Size:        476     627       5     629

  Fragments arranged by size: 

                 629     627     476       5
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1526    1676    1737 
   Size:       1526     150      61

  Fragments arranged by size: 

                1526     150      61
 
BsaAI yAC'GTr

Cuts at:      0     357    1119    1737 
   Size:        357     762     618

  Fragments arranged by size: 

                 762     618     357
 
BsaHI Gr'CG_yC

Cuts at:      0     501    1053    1091    1158    1447    1737 
   Size:        501     552      38      67     289     290

  Fragments arranged by size: 

                 552     501     290     289      67      38
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1118    1148    1563    1593    1737 
   Size:       1118      30     415      30     144

  Fragments arranged by size: 

                1118     415     144      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      63     464     761     764    1737 
   Size:         63     401     297       3     973

  Fragments arranged by size: 

                 973     401     297      63       3
 
BseYI C'CCAG_C

Cuts at:      0    1492    1502    1737 
   Size:       1492      10     235

  Fragments arranged by size: 

                1492     235      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     867    1737 
   Size:        379     488     870

  Fragments arranged by size: 

                 870     488     379
 
BsiHKAI G_wGCw'C

Cuts at:      0     408     754    1406    1737 
   Size:        408     346     652     331

  Fragments arranged by size: 

                 652     408     346     331
 
BsmI GAATG_Cn'

Cuts at:      0     445    1737 
   Size:        445    1292
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     951    1737 
   Size:        951     786
 
Bsp1286I G_dGCh'C

Cuts at:      0     408     695     715     754     830    1406    1469    1510 
   Size:        408     287      20      39      76     576      63      41

Cuts at:   1510    1737 
   Size:        227

  Fragments arranged by size: 

                 576     408     287     227      76      63      41      39
                  20
 
BspHI T'CATG_A

Cuts at:      0    1545    1737 
   Size:       1545     192
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     228     607     854     999    1737 
   Size:        228     379     247     145     738

  Fragments arranged by size: 

                 738     379     247     228     145
 
BsrBI CCG'CTC

Cuts at:      0      36     743     899    1058    1659    1737 
   Size:         36     707     156     159     601      78

  Fragments arranged by size: 

                 707     601     159     156      78      36
 
BsrDI GCAATG_nn'

Cuts at:      0    1698    1737 
   Size:       1698      39
 
BsrFI r'CCGG_y

Cuts at:      0    1443    1737 
   Size:       1443     294
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1726    1737 
   Size:       1726      11
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1069    1737 
   Size:       1069     668
 
BstYI r'GATC_y

Cuts at:      0      17    1737 
   Size:         17    1720
 
Bsu36I CC'TnA_GG

Cuts at:      0     521    1737 
   Size:        521    1216
 
BtgI C'CryG_G

Cuts at:      0    1153    1330    1677    1737 
   Size:       1153     177     347      60

  Fragments arranged by size: 

                1153     347     177      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1206    1737 
   Size:       1206     531
 
BtsI GCAGTG_nn'

Cuts at:      0     367     515    1183    1366    1737 
   Size:        367     148     668     183     371

  Fragments arranged by size: 

                 668     371     367     183     148
 
DraIII CAC_nnn'GTG

Cuts at:      0     542    1737 
   Size:        542    1195
 
EaeI y'GGCC_r

Cuts at:      0    1333    1674    1737 
   Size:       1333     341      63

  Fragments arranged by size: 

                1333     341      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     819     884    1724    1737 
   Size:        392     427      65     840      13

  Fragments arranged by size: 

                 840     427     392      65      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1352    1737 
   Size:       1352     385
 
EcoO109I rG'GnC_Cy

Cuts at:      0      58    1506    1737 
   Size:         58    1448     231

  Fragments arranged by size: 

                1448     231      58
 
HaeII r_GCGC'y

Cuts at:      0    1094    1450    1737 
   Size:       1094     356     287

  Fragments arranged by size: 

                1094     356     287
 
HindIII A'AGCT_T

Cuts at:      0    1140    1737 
   Size:       1140     597
 
KasI G'GCGC_C

Cuts at:      0    1090    1446    1737 
   Size:       1090     356     291

  Fragments arranged by size: 

                1090     356     291
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      63    1348    1737 
   Size:         63    1285     389

  Fragments arranged by size: 

                1285     389      63
 
MscI TGG'CCA

Cuts at:      0    1676    1737 
   Size:       1676      61
 
MslI CAynn'nnrTG

Cuts at:      0     211     277    1023    1383    1737 
   Size:        211      66     746     360     354

  Fragments arranged by size: 

                 746     360     354     211      66
 
MspA1I CmG'CkG

Cuts at:      0     647     803    1332    1737 
   Size:        647     156     529     405

  Fragments arranged by size: 

                 647     529     405     156
 
NaeI GCC'GGC

Cuts at:      0    1445    1737 
   Size:       1445     292
 
NarI GG'CG_CC

Cuts at:      0    1091    1447    1737 
   Size:       1091     356     290

  Fragments arranged by size: 

                1091     356     290
 
NcoI C'CATG_G

Cuts at:      0    1677    1737 
   Size:       1677      60
 
NgoMIV G'CCGG_C

Cuts at:      0    1443    1737 
   Size:       1443     294
 
NsiI A_TGCA'T

Cuts at:      0    1240    1737 
   Size:       1240     497
 
PasI CC'CwG_GG

Cuts at:      0     708    1354    1737 
   Size:        708     646     383

  Fragments arranged by size: 

                 708     646     383
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     105    1737 
   Size:        105    1632
 
PfoI T'CCnGG_A

Cuts at:      0     667    1737 
   Size:        667    1070
 
PpuMI rG'GwC_Cy

Cuts at:      0      58    1737 
   Size:         58    1679
 
PshAI GACnn'nnGTC

Cuts at:      0    1169    1737 
   Size:       1169     568
 
PspOMI G'GGCC_C

Cuts at:      0    1506    1737 
   Size:       1506     231
 
PstI C_TGCA'G

Cuts at:      0     510     788    1190    1394    1474    1737 
   Size:        510     278     402     204      80     263

  Fragments arranged by size: 

                 510     402     278     263     204      80
 
PvuII CAG'CTG

Cuts at:      0     647     803    1737 
   Size:        647     156     934

  Fragments arranged by size: 

                 934     647     156
 
SacII CC_GC'GG

Cuts at:      0    1333    1737 
   Size:       1333     404
 
SanDI GG'GwC_CC

Cuts at:      0      58    1737 
   Size:         58    1679
 
SbfI CC_TGCA'GG

Cuts at:      0    1474    1737 
   Size:       1474     263
 
SfcI C'TryA_G

Cuts at:      0     506     561     639     784    1186    1390    1470    1737 
   Size:        506      55      78     145     402     204      80     267

  Fragments arranged by size: 

                 506     402     267     204     145      80      78      55
            
 
SfoI GGC'GCC

Cuts at:      0    1092    1448    1737 
   Size:       1092     356     289

  Fragments arranged by size: 

                1092     356     289
 
StuI AGG'CCT

Cuts at:      0     633    1737 
   Size:        633    1104
 
StyI C'CwwG_G

Cuts at:      0    1536    1677    1686    1737 
   Size:       1536     141       9      51

  Fragments arranged by size: 

                1536     141      51       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     289     553     653    1421    1737 
   Size:        289     264     100     768     316

  Fragments arranged by size: 

                 768     316     289     264     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1118    1150    1563    1595    1737 
   Size:       1118      32     413      32     142

  Fragments arranged by size: 

                1118     413     142      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1245    1737 
   Size:       1245     492
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     715    1737 
   Size:        715    1022
 
XmnI GAAnn'nnTTC

Cuts at:      0    1307    1737 
   Size:       1307     430
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI    AvrII
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI  Eco57MI    EcoNI
 EcoO109I    HaeII  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NsiI     PasI    PflMI     PfoI
    PpuMI    PshAI   PspOMI     PstI    PvuII    SacII    SanDI     SbfI
     SfcI     SfoI     StuI     StyI    TaqII     TstI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI     BaeI
    BamHI     BbsI     BclI     BglI    BglII     BlpI     BmtI    BpuEI
    BsaBI    BsaWI    BsiEI    BsiWI    BspEI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NspI     PacI     PciI     PmeI     PmlI
     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I
     XhoI     XmaI     ZraI
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